Characterization of insect-specific Culex flavivirus (Flaviviridae) nucleotide sequences in mosquitoes from urban parks in São Paulo, Brazil
ABSTRACT Background: Despite their worldwide occurrence, the distribution and role of insect-specific flaviviruses (ISFs) remain unclear. Methods: We evaluated the presence of ISFs in mosquitoes collected in São Paulo, Brazil, using reverse transcription and semi-nested polymerase chain reaction...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Medicina Tropical (SBMT)
2022-09-01
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| Series: | Revista da Sociedade Brasileira de Medicina Tropical |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822022000100624&tlng=en |
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| author | Thaís de Moura Coletti Camila Malta Romano Paulo Roberto Urbinatti Renildo Souza Teixeira Leila Weiss de Almeida Pedrosa Marcello Schiavo Nardi Delsio Natal Antônio Charlys da Costa Jaqueline Goes de Jesus Ingra Morales Claro Ester Cerdeira Sabino Steven S. Witkin Mauro Toledo Marrelli Licia Natal Fernandes |
| author_facet | Thaís de Moura Coletti Camila Malta Romano Paulo Roberto Urbinatti Renildo Souza Teixeira Leila Weiss de Almeida Pedrosa Marcello Schiavo Nardi Delsio Natal Antônio Charlys da Costa Jaqueline Goes de Jesus Ingra Morales Claro Ester Cerdeira Sabino Steven S. Witkin Mauro Toledo Marrelli Licia Natal Fernandes |
| author_sort | Thaís de Moura Coletti |
| collection | DOAJ |
| description | ABSTRACT Background: Despite their worldwide occurrence, the distribution and role of insect-specific flaviviruses (ISFs) remain unclear. Methods: We evaluated the presence of ISFs in mosquitoes collected in São Paulo, Brazil, using reverse transcription and semi-nested polymerase chain reaction (PCR). Some of the positive samples were subjected to nanopore sequencing. Results: Twelve mosquito pools (2.8%) tested positive for flavivirus infection. Nanopore sequencing was successfully performed on six samples. Phylogenetic analysis grouped these sequences into genotype 2 of Culex flavivirus (CxFV). Conclusions: The identification of CxFV genotype 2 at new locations in São Paulo highlights the importance of understanding the role of ISFs in mosquito vector competence. |
| first_indexed | 2024-04-11T11:34:32Z |
| format | Article |
| id | doaj.art-65c82a69971a4ee5a706b6284926fa8d |
| institution | Directory Open Access Journal |
| issn | 1678-9849 |
| language | English |
| last_indexed | 2024-04-11T11:34:32Z |
| publishDate | 2022-09-01 |
| publisher | Sociedade Brasileira de Medicina Tropical (SBMT) |
| record_format | Article |
| series | Revista da Sociedade Brasileira de Medicina Tropical |
| spelling | doaj.art-65c82a69971a4ee5a706b6284926fa8d2022-12-22T04:26:00ZengSociedade Brasileira de Medicina Tropical (SBMT)Revista da Sociedade Brasileira de Medicina Tropical1678-98492022-09-015510.1590/0037-8682-0067-2022Characterization of insect-specific Culex flavivirus (Flaviviridae) nucleotide sequences in mosquitoes from urban parks in São Paulo, BrazilThaís de Moura Colettihttps://orcid.org/0000-0003-1693-7984Camila Malta Romanohttps://orcid.org/0000-0003-4550-1987Paulo Roberto Urbinattihttps://orcid.org/0000-0001-5757-9576Renildo Souza Teixeirahttps://orcid.org/0000-0002-8576-8996Leila Weiss de Almeida Pedrosahttps://orcid.org/0000-0001-7665-229XMarcello Schiavo Nardihttps://orcid.org/0000-0001-6234-3365Delsio Natalhttps://orcid.org/0000-0002-8717-8578Antônio Charlys da Costahttps://orcid.org/0000-0001-5516-5177Jaqueline Goes de Jesushttps://orcid.org/0000-0002-5404-272XIngra Morales Clarohttps://orcid.org/0000-0001-8637-2910Ester Cerdeira Sabinohttps://orcid.org/0000-0003-2623-5126Steven S. Witkinhttps://orcid.org/0000-0002-3502-3929Mauro Toledo Marrellihttps://orcid.org/0000-0002-0312-8777Licia Natal Fernandeshttps://orcid.org/0000-0001-8008-1338ABSTRACT Background: Despite their worldwide occurrence, the distribution and role of insect-specific flaviviruses (ISFs) remain unclear. Methods: We evaluated the presence of ISFs in mosquitoes collected in São Paulo, Brazil, using reverse transcription and semi-nested polymerase chain reaction (PCR). Some of the positive samples were subjected to nanopore sequencing. Results: Twelve mosquito pools (2.8%) tested positive for flavivirus infection. Nanopore sequencing was successfully performed on six samples. Phylogenetic analysis grouped these sequences into genotype 2 of Culex flavivirus (CxFV). Conclusions: The identification of CxFV genotype 2 at new locations in São Paulo highlights the importance of understanding the role of ISFs in mosquito vector competence.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822022000100624&tlng=enCulex flavivirusInsect-specific flavivirusMosquitoUrban park |
| spellingShingle | Thaís de Moura Coletti Camila Malta Romano Paulo Roberto Urbinatti Renildo Souza Teixeira Leila Weiss de Almeida Pedrosa Marcello Schiavo Nardi Delsio Natal Antônio Charlys da Costa Jaqueline Goes de Jesus Ingra Morales Claro Ester Cerdeira Sabino Steven S. Witkin Mauro Toledo Marrelli Licia Natal Fernandes Characterization of insect-specific Culex flavivirus (Flaviviridae) nucleotide sequences in mosquitoes from urban parks in São Paulo, Brazil Revista da Sociedade Brasileira de Medicina Tropical Culex flavivirus Insect-specific flavivirus Mosquito Urban park |
| title | Characterization of insect-specific Culex flavivirus (Flaviviridae) nucleotide sequences in mosquitoes from urban parks in São Paulo, Brazil |
| title_full | Characterization of insect-specific Culex flavivirus (Flaviviridae) nucleotide sequences in mosquitoes from urban parks in São Paulo, Brazil |
| title_fullStr | Characterization of insect-specific Culex flavivirus (Flaviviridae) nucleotide sequences in mosquitoes from urban parks in São Paulo, Brazil |
| title_full_unstemmed | Characterization of insect-specific Culex flavivirus (Flaviviridae) nucleotide sequences in mosquitoes from urban parks in São Paulo, Brazil |
| title_short | Characterization of insect-specific Culex flavivirus (Flaviviridae) nucleotide sequences in mosquitoes from urban parks in São Paulo, Brazil |
| title_sort | characterization of insect specific culex flavivirus flaviviridae nucleotide sequences in mosquitoes from urban parks in sao paulo brazil |
| topic | Culex flavivirus Insect-specific flavivirus Mosquito Urban park |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822022000100624&tlng=en |
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