Surface Ultrastructure and Molecular Studies of Clinostomum complanatum (Rudolphi, 1814) Braun, 1899 (Trematoda: Clinostomidae) Metacercariae in some Freshwater Fishes from Sulaimani Province, Iraq
Clinostomum is a trematode genus in the family Clinostomidae. The mentioned trematode species is parasitizing many fish species as intermediate hosts, while piscivorous birds and mammals are the main definitive hosts. Total of 58 metacercariae of Clinostomum larvae were dissected out from 25 infecte...
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| Format: | Article |
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University of Basrah
2023-12-01
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| Series: | Maǧallaẗ al-baṣraẗ al-ʻulūm al-zirāʻiyyaẗ |
| Subjects: | |
| Online Access: | https://bjas.bajas.edu.iq/index.php/bjas/article/view/1155 |
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| author | Younis S. Abdullah Samir J. Bilal Taeeb A. Hama Soor Yousif O. Mohammad |
| author_facet | Younis S. Abdullah Samir J. Bilal Taeeb A. Hama Soor Yousif O. Mohammad |
| author_sort | Younis S. Abdullah |
| collection | DOAJ |
| description | Clinostomum is a trematode genus in the family Clinostomidae. The mentioned trematode species is parasitizing many fish species as intermediate hosts, while piscivorous birds and mammals are the main definitive hosts. Total of 58 metacercariae of Clinostomum larvae were dissected out from 25 infected fish specimens from eight different species from a number of water bodies of Sulaimani Province, Kurdistan Region, Iraq. In this investigation, 959 fish specimens were collected. These includes five cyprinid species: Capoeta trutta (Heckel), C. umbla (Heckel), Carasobarbus kosswigi (Ladiges), Cyprinion macrostomum Heckel and Garra rufa (Heckel), two leuciscid species: Alburnus sellal Heckel and Squalius lepidus Heckel and the mugilid fish Planiliza abu (Heckel). The prevalence of infection for each of these species was 1.8%, 5%, 20%, 1.5%, 1.7%, 4.9%, 4.9% and 1.3%, respectively. The morphology of the Clinostomum metacercariae was studied by using a compound microscope; their ultra-morphology was evaluated with a scanning electron microscopy (SEM); and the molecular analysis were performed by amplifying, sequencing and comparing the ITS1-5.8S-ITS2 and 28S rDNA gene loci from isolated Clinostomum metacercariae. The obtained sequences confirmed that all metacercariae of the Clinostomum, collected during the present study, represented with C. complanatum (Rudolphi, 1814) based on percent identity with sequences in the GenBank subject database. The molecular characterisations of the C. complanatum metacercariae in the present study were deposited in GenBank NCBI.
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| first_indexed | 2024-03-08T18:32:43Z |
| format | Article |
| id | doaj.art-65c94b9d0c09476c8dea75e5901ae307 |
| institution | Directory Open Access Journal |
| issn | 1814-5868 2520-0860 |
| language | English |
| last_indexed | 2024-03-08T18:32:43Z |
| publishDate | 2023-12-01 |
| publisher | University of Basrah |
| record_format | Article |
| series | Maǧallaẗ al-baṣraẗ al-ʻulūm al-zirāʻiyyaẗ |
| spelling | doaj.art-65c94b9d0c09476c8dea75e5901ae3072023-12-29T19:50:08ZengUniversity of BasrahMaǧallaẗ al-baṣraẗ al-ʻulūm al-zirāʻiyyaẗ1814-58682520-08602023-12-01362Surface Ultrastructure and Molecular Studies of Clinostomum complanatum (Rudolphi, 1814) Braun, 1899 (Trematoda: Clinostomidae) Metacercariae in some Freshwater Fishes from Sulaimani Province, IraqYounis S. Abdullah0Samir J. Bilal1Taeeb A. Hama Soor2Yousif O. Mohammad3Medical Laboratory Dept, College of Health & Medical Technology, Sulaimani Polytechnic Univ., IraqDepartment of Fish Resources and Aquatic Animals, College of Agricultural Engineering Sciences, Salahaddin University- Erbil, IraqMedical Laboratory Dept, College of Health & Medical Technology, Sulaimani Polytechnic Univ., IraqDepartment of Geology, College of Science, University of Sulaimani, IraqClinostomum is a trematode genus in the family Clinostomidae. The mentioned trematode species is parasitizing many fish species as intermediate hosts, while piscivorous birds and mammals are the main definitive hosts. Total of 58 metacercariae of Clinostomum larvae were dissected out from 25 infected fish specimens from eight different species from a number of water bodies of Sulaimani Province, Kurdistan Region, Iraq. In this investigation, 959 fish specimens were collected. These includes five cyprinid species: Capoeta trutta (Heckel), C. umbla (Heckel), Carasobarbus kosswigi (Ladiges), Cyprinion macrostomum Heckel and Garra rufa (Heckel), two leuciscid species: Alburnus sellal Heckel and Squalius lepidus Heckel and the mugilid fish Planiliza abu (Heckel). The prevalence of infection for each of these species was 1.8%, 5%, 20%, 1.5%, 1.7%, 4.9%, 4.9% and 1.3%, respectively. The morphology of the Clinostomum metacercariae was studied by using a compound microscope; their ultra-morphology was evaluated with a scanning electron microscopy (SEM); and the molecular analysis were performed by amplifying, sequencing and comparing the ITS1-5.8S-ITS2 and 28S rDNA gene loci from isolated Clinostomum metacercariae. The obtained sequences confirmed that all metacercariae of the Clinostomum, collected during the present study, represented with C. complanatum (Rudolphi, 1814) based on percent identity with sequences in the GenBank subject database. The molecular characterisations of the C. complanatum metacercariae in the present study were deposited in GenBank NCBI. https://bjas.bajas.edu.iq/index.php/bjas/article/view/1155DigeneaFreshwater fishesGeneticsIraqMetacercariaYellow grub disease |
| spellingShingle | Younis S. Abdullah Samir J. Bilal Taeeb A. Hama Soor Yousif O. Mohammad Surface Ultrastructure and Molecular Studies of Clinostomum complanatum (Rudolphi, 1814) Braun, 1899 (Trematoda: Clinostomidae) Metacercariae in some Freshwater Fishes from Sulaimani Province, Iraq Maǧallaẗ al-baṣraẗ al-ʻulūm al-zirāʻiyyaẗ Digenea Freshwater fishes Genetics Iraq Metacercaria Yellow grub disease |
| title | Surface Ultrastructure and Molecular Studies of Clinostomum complanatum (Rudolphi, 1814) Braun, 1899 (Trematoda: Clinostomidae) Metacercariae in some Freshwater Fishes from Sulaimani Province, Iraq |
| title_full | Surface Ultrastructure and Molecular Studies of Clinostomum complanatum (Rudolphi, 1814) Braun, 1899 (Trematoda: Clinostomidae) Metacercariae in some Freshwater Fishes from Sulaimani Province, Iraq |
| title_fullStr | Surface Ultrastructure and Molecular Studies of Clinostomum complanatum (Rudolphi, 1814) Braun, 1899 (Trematoda: Clinostomidae) Metacercariae in some Freshwater Fishes from Sulaimani Province, Iraq |
| title_full_unstemmed | Surface Ultrastructure and Molecular Studies of Clinostomum complanatum (Rudolphi, 1814) Braun, 1899 (Trematoda: Clinostomidae) Metacercariae in some Freshwater Fishes from Sulaimani Province, Iraq |
| title_short | Surface Ultrastructure and Molecular Studies of Clinostomum complanatum (Rudolphi, 1814) Braun, 1899 (Trematoda: Clinostomidae) Metacercariae in some Freshwater Fishes from Sulaimani Province, Iraq |
| title_sort | surface ultrastructure and molecular studies of clinostomum complanatum rudolphi 1814 braun 1899 trematoda clinostomidae metacercariae in some freshwater fishes from sulaimani province iraq |
| topic | Digenea Freshwater fishes Genetics Iraq Metacercaria Yellow grub disease |
| url | https://bjas.bajas.edu.iq/index.php/bjas/article/view/1155 |
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