The Glypican 3-Hosted Murine Mir717 Gene: Sequence Conservation, Seed Region Polymorphisms and Putative Targets
<p><i>Mir717</i> (<i>mmu-mir-717</i>) was first reported in mouse and resides in the intron 3 of glypican 3 (<i>Gpc3</i>) gene. Our present study revealed that this microRNA (miRNA) gene is conserved among 26 mammalian species and harbors polymorphic sites w...
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| Format: | Article |
| Language: | English |
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Ivyspring International Publisher
2010-01-01
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| Series: | International Journal of Biological Sciences |
| Online Access: | http://www.biolsci.org/v06p0769.htm |
| _version_ | 1819058636807208960 |
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| author | Tanja Kunej, Dasa Jevsinek Skok, Simon Horvat, Peter Dovc, Zhihua Jiang |
| author_facet | Tanja Kunej, Dasa Jevsinek Skok, Simon Horvat, Peter Dovc, Zhihua Jiang |
| author_sort | Tanja Kunej, Dasa Jevsinek Skok, Simon Horvat, Peter Dovc, Zhihua Jiang |
| collection | DOAJ |
| description | <p><i>Mir717</i> (<i>mmu-mir-717</i>) was first reported in mouse and resides in the intron 3 of glypican 3 (<i>Gpc3</i>) gene. Our present study revealed that this microRNA (miRNA) gene is conserved among 26 mammalian species and harbors polymorphic sites within the mature seed region in mice. Our finding represents a rare four layer genomic overlap consisting of growth associated quantitative trait locus (QTL), body mass associated <i>Gpc3</i> gene, highly conserved miRNA gene and mature miRNA seed single nucleotide polymorphism (SNP) identified in the lean mouse strain 129/Sv. Additionally, genes potentially targeted by Mir717 include 91 genes associated with obesity and related phenotypes in mammals. Our analysis provides a basis for further experiments to causally connect the identified SNP and <i>Mir717</i> gene itself to obesity regulation. Furthermore, our bioinformatics analysis now enables functional annotation of <i>Mir717</i> orthologs in other species, thus determining the effect of its target genes on fat-related traits.</p> |
| first_indexed | 2024-12-21T13:58:21Z |
| format | Article |
| id | doaj.art-65cab1ff651c4d7e8a263160263927b5 |
| institution | Directory Open Access Journal |
| issn | 1449-2288 |
| language | English |
| last_indexed | 2024-12-21T13:58:21Z |
| publishDate | 2010-01-01 |
| publisher | Ivyspring International Publisher |
| record_format | Article |
| series | International Journal of Biological Sciences |
| spelling | doaj.art-65cab1ff651c4d7e8a263160263927b52022-12-21T19:01:27ZengIvyspring International PublisherInternational Journal of Biological Sciences1449-22882010-01-0167769772The Glypican 3-Hosted Murine Mir717 Gene: Sequence Conservation, Seed Region Polymorphisms and Putative TargetsTanja Kunej, Dasa Jevsinek Skok, Simon Horvat, Peter Dovc, Zhihua Jiang<p><i>Mir717</i> (<i>mmu-mir-717</i>) was first reported in mouse and resides in the intron 3 of glypican 3 (<i>Gpc3</i>) gene. Our present study revealed that this microRNA (miRNA) gene is conserved among 26 mammalian species and harbors polymorphic sites within the mature seed region in mice. Our finding represents a rare four layer genomic overlap consisting of growth associated quantitative trait locus (QTL), body mass associated <i>Gpc3</i> gene, highly conserved miRNA gene and mature miRNA seed single nucleotide polymorphism (SNP) identified in the lean mouse strain 129/Sv. Additionally, genes potentially targeted by Mir717 include 91 genes associated with obesity and related phenotypes in mammals. Our analysis provides a basis for further experiments to causally connect the identified SNP and <i>Mir717</i> gene itself to obesity regulation. Furthermore, our bioinformatics analysis now enables functional annotation of <i>Mir717</i> orthologs in other species, thus determining the effect of its target genes on fat-related traits.</p>http://www.biolsci.org/v06p0769.htm |
| spellingShingle | Tanja Kunej, Dasa Jevsinek Skok, Simon Horvat, Peter Dovc, Zhihua Jiang The Glypican 3-Hosted Murine Mir717 Gene: Sequence Conservation, Seed Region Polymorphisms and Putative Targets International Journal of Biological Sciences |
| title | The Glypican 3-Hosted Murine Mir717 Gene: Sequence Conservation, Seed Region Polymorphisms and Putative Targets |
| title_full | The Glypican 3-Hosted Murine Mir717 Gene: Sequence Conservation, Seed Region Polymorphisms and Putative Targets |
| title_fullStr | The Glypican 3-Hosted Murine Mir717 Gene: Sequence Conservation, Seed Region Polymorphisms and Putative Targets |
| title_full_unstemmed | The Glypican 3-Hosted Murine Mir717 Gene: Sequence Conservation, Seed Region Polymorphisms and Putative Targets |
| title_short | The Glypican 3-Hosted Murine Mir717 Gene: Sequence Conservation, Seed Region Polymorphisms and Putative Targets |
| title_sort | glypican 3 hosted murine mir717 gene sequence conservation seed region polymorphisms and putative targets |
| url | http://www.biolsci.org/v06p0769.htm |
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