Disrupted brain mitochondrial morphology after in vivo hydrogen sulfide exposure
Abstract Changes in mitochondrial dynamics are often associated with dietary patterns, medical treatments, xenobiotics, and diseases. Toxic exposures to hydrogen sulfide (H2S) harm mitochondria by inhibiting Complex IV and via other mechanisms. However, changes in mitochondrial dynamics, including m...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-44807-y |
| _version_ | 1797453276006842368 |
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| author | Wilson K. Rumbeiha Dong-Suk Kim Angela Min Maya Nair Cecilia Giulivi |
| author_facet | Wilson K. Rumbeiha Dong-Suk Kim Angela Min Maya Nair Cecilia Giulivi |
| author_sort | Wilson K. Rumbeiha |
| collection | DOAJ |
| description | Abstract Changes in mitochondrial dynamics are often associated with dietary patterns, medical treatments, xenobiotics, and diseases. Toxic exposures to hydrogen sulfide (H2S) harm mitochondria by inhibiting Complex IV and via other mechanisms. However, changes in mitochondrial dynamics, including morphology following acute exposure to H2S, are not yet fully understood. This study followed mitochondrial morphology changes over time after a single acute LCt50 dose of H2S by examining electron microscopy thalami images of surviving mice. Our findings revealed that within the initial 48 h after H2S exposure, mitochondrial morphology was impaired by H2S, supported by the disruption and scarcity of the cristae, which are required to enhance the surface area for ATP production. At the 72-h mark point, a spectrum of morphological cellular changes was observed, and the disordered mitochondrial network, accompanied by the probable disruption of mitophagy, was tied to changes in mitochondrial shape. In summary, this study sheds light on how acute exposure to high levels of H2S triggers alterations in mitochondrial shape and structure as early as 24 h that become more evident at 72 h post-exposure. These findings underscore the impact of H2S on mitochondrial function and overall cellular health. |
| first_indexed | 2024-03-09T15:20:30Z |
| format | Article |
| id | doaj.art-65cb3b86e898453da45676bd1bc2e271 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-09T15:20:30Z |
| publishDate | 2023-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-65cb3b86e898453da45676bd1bc2e2712023-11-26T12:51:04ZengNature PortfolioScientific Reports2045-23222023-10-0113111410.1038/s41598-023-44807-yDisrupted brain mitochondrial morphology after in vivo hydrogen sulfide exposureWilson K. Rumbeiha0Dong-Suk Kim1Angela Min2Maya Nair3Cecilia Giulivi4Department of Molecular Biosciences, School of Veterinary Medicine, University of California DavisDepartment of Molecular Biosciences, School of Veterinary Medicine, University of California DavisDepartment of Molecular Biosciences, School of Veterinary Medicine, University of California DavisDepartment of Molecular Biosciences, School of Veterinary Medicine, University of California DavisDepartment of Molecular Biosciences, School of Veterinary Medicine, University of California DavisAbstract Changes in mitochondrial dynamics are often associated with dietary patterns, medical treatments, xenobiotics, and diseases. Toxic exposures to hydrogen sulfide (H2S) harm mitochondria by inhibiting Complex IV and via other mechanisms. However, changes in mitochondrial dynamics, including morphology following acute exposure to H2S, are not yet fully understood. This study followed mitochondrial morphology changes over time after a single acute LCt50 dose of H2S by examining electron microscopy thalami images of surviving mice. Our findings revealed that within the initial 48 h after H2S exposure, mitochondrial morphology was impaired by H2S, supported by the disruption and scarcity of the cristae, which are required to enhance the surface area for ATP production. At the 72-h mark point, a spectrum of morphological cellular changes was observed, and the disordered mitochondrial network, accompanied by the probable disruption of mitophagy, was tied to changes in mitochondrial shape. In summary, this study sheds light on how acute exposure to high levels of H2S triggers alterations in mitochondrial shape and structure as early as 24 h that become more evident at 72 h post-exposure. These findings underscore the impact of H2S on mitochondrial function and overall cellular health.https://doi.org/10.1038/s41598-023-44807-y |
| spellingShingle | Wilson K. Rumbeiha Dong-Suk Kim Angela Min Maya Nair Cecilia Giulivi Disrupted brain mitochondrial morphology after in vivo hydrogen sulfide exposure Scientific Reports |
| title | Disrupted brain mitochondrial morphology after in vivo hydrogen sulfide exposure |
| title_full | Disrupted brain mitochondrial morphology after in vivo hydrogen sulfide exposure |
| title_fullStr | Disrupted brain mitochondrial morphology after in vivo hydrogen sulfide exposure |
| title_full_unstemmed | Disrupted brain mitochondrial morphology after in vivo hydrogen sulfide exposure |
| title_short | Disrupted brain mitochondrial morphology after in vivo hydrogen sulfide exposure |
| title_sort | disrupted brain mitochondrial morphology after in vivo hydrogen sulfide exposure |
| url | https://doi.org/10.1038/s41598-023-44807-y |
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