A novel mechanism of gland formation in zebrafish involving transdifferentiation of renal epithelial cells and live cell extrusion
Transdifferentiation is the poorly understood phenomenon whereby a terminally differentiated cell acquires a completely new identity. Here, we describe a rare example of a naturally occurring transdifferentiation event in zebrafish in which kidney distal tubule epithelial cells are converted into an...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2018-11-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/38911 |
| _version_ | 1828384474206830592 |
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| author | Richard W Naylor Hao-Han G Chang Sarah Qubisi Alan J Davidson |
| author_facet | Richard W Naylor Hao-Han G Chang Sarah Qubisi Alan J Davidson |
| author_sort | Richard W Naylor |
| collection | DOAJ |
| description | Transdifferentiation is the poorly understood phenomenon whereby a terminally differentiated cell acquires a completely new identity. Here, we describe a rare example of a naturally occurring transdifferentiation event in zebrafish in which kidney distal tubule epithelial cells are converted into an endocrine gland known as the Corpuscles of Stannius (CS). We find that this process requires Notch signalling and is associated with the cytoplasmic sequestration of the Hnf1b transcription factor, a master-regulator of renal tubule fate. A deficiency in the Irx3b transcription factor results in ectopic transdifferentiation of distal tubule cells to a CS identity but in a Notch-dependent fashion. Using live-cell imaging we show that CS cells undergo apical constriction en masse and are then extruded from the tubule to form a distinct organ. This system provides a valuable new model to understand the molecular and morphological basis of transdifferentiation and will advance efforts to exploit this rare phenomenon therapeutically. |
| first_indexed | 2024-12-10T05:04:05Z |
| format | Article |
| id | doaj.art-65cd9f5935134c4dbfe22950007a3c78 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-12-10T05:04:05Z |
| publishDate | 2018-11-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-65cd9f5935134c4dbfe22950007a3c782022-12-22T02:01:19ZengeLife Sciences Publications LtdeLife2050-084X2018-11-01710.7554/eLife.38911A novel mechanism of gland formation in zebrafish involving transdifferentiation of renal epithelial cells and live cell extrusionRichard W Naylor0https://orcid.org/0000-0003-2901-7677Hao-Han G Chang1https://orcid.org/0000-0001-8023-6862Sarah Qubisi2Alan J Davidson3https://orcid.org/0000-0002-5732-1193Department of Molecular Medicine and Pathology, University of Auckland, Auckland, New ZealandDepartment of Molecular Medicine and Pathology, University of Auckland, Auckland, New ZealandDepartment of Molecular Medicine and Pathology, University of Auckland, Auckland, New ZealandDepartment of Molecular Medicine and Pathology, University of Auckland, Auckland, New ZealandTransdifferentiation is the poorly understood phenomenon whereby a terminally differentiated cell acquires a completely new identity. Here, we describe a rare example of a naturally occurring transdifferentiation event in zebrafish in which kidney distal tubule epithelial cells are converted into an endocrine gland known as the Corpuscles of Stannius (CS). We find that this process requires Notch signalling and is associated with the cytoplasmic sequestration of the Hnf1b transcription factor, a master-regulator of renal tubule fate. A deficiency in the Irx3b transcription factor results in ectopic transdifferentiation of distal tubule cells to a CS identity but in a Notch-dependent fashion. Using live-cell imaging we show that CS cells undergo apical constriction en masse and are then extruded from the tubule to form a distinct organ. This system provides a valuable new model to understand the molecular and morphological basis of transdifferentiation and will advance efforts to exploit this rare phenomenon therapeutically.https://elifesciences.org/articles/38911transdifferentiationkidneyendocrine glandHnf1bIrx3bNotch |
| spellingShingle | Richard W Naylor Hao-Han G Chang Sarah Qubisi Alan J Davidson A novel mechanism of gland formation in zebrafish involving transdifferentiation of renal epithelial cells and live cell extrusion eLife transdifferentiation kidney endocrine gland Hnf1b Irx3b Notch |
| title | A novel mechanism of gland formation in zebrafish involving transdifferentiation of renal epithelial cells and live cell extrusion |
| title_full | A novel mechanism of gland formation in zebrafish involving transdifferentiation of renal epithelial cells and live cell extrusion |
| title_fullStr | A novel mechanism of gland formation in zebrafish involving transdifferentiation of renal epithelial cells and live cell extrusion |
| title_full_unstemmed | A novel mechanism of gland formation in zebrafish involving transdifferentiation of renal epithelial cells and live cell extrusion |
| title_short | A novel mechanism of gland formation in zebrafish involving transdifferentiation of renal epithelial cells and live cell extrusion |
| title_sort | novel mechanism of gland formation in zebrafish involving transdifferentiation of renal epithelial cells and live cell extrusion |
| topic | transdifferentiation kidney endocrine gland Hnf1b Irx3b Notch |
| url | https://elifesciences.org/articles/38911 |
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