Attenuation of influenza virus infectivity with herbal-marine compound (HESA-A): an in vitro study in MDCK cells
<p>Abstract</p> <p>Background</p> <p>The influenza virus is still one of the most important respiratory risks affecting humans which require effective treatments. In this case, traditional medications are of interest. HESA-A is an active natural biological compound from...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2012-02-01
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| Series: | Virology Journal |
| Subjects: | |
| Online Access: | http://www.virologyj.com/content/9/1/44 |
| _version_ | 1818786904093491200 |
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| author | Mehrbod Parvaneh Ideris Aini Omar Abdul Rahman Hair-Bejo Mohd Tan Sheau Wei Kheiri Masoumeh Tabatabaian Mansoureh |
| author_facet | Mehrbod Parvaneh Ideris Aini Omar Abdul Rahman Hair-Bejo Mohd Tan Sheau Wei Kheiri Masoumeh Tabatabaian Mansoureh |
| author_sort | Mehrbod Parvaneh |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The influenza virus is still one of the most important respiratory risks affecting humans which require effective treatments. In this case, traditional medications are of interest. HESA-A is an active natural biological compound from herbal-marine origin. Previous studies have reported that the therapeutic properties of HESA-A are able to treat psoriasis vulgaris and cancers. However, no antiviral properties have been reported.</p> <p>Methods</p> <p>This study was designed to investigate the potential antiviral properties of HESA-A and its effects in modulating TNF-α and IL-6 cytokine levels. HESA-A was prepared in normal saline as a stock solution (0.8 mg/ml, pH = 7.4). Percentages of cell survival when exposed to different concentrations of HESA-A at different time intervals was determined by MTT assay. To study the potential antiviral activity of HESA-A, Madin-Darby Canine Kidney (MDCK) cells were treated with the effective concentration (EC<sub>50</sub>) of HESA-A (0.025 mg/ml) and 100 TCID<sub>50</sub>/0.1 ml of virus sample under different types of exposure.</p> <p>Results</p> <p>Based on the MTT method and hemagglutination assay (HA), HESA-A is capable of improving cell viability to 31% and decreasing HA titre to almost 99% in co-penetration exposures. In addition, based on quantitative real-time PCR (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA), it was found that HESA-A causes decrements in TNF-α and IL-6 cytokine expressions, which was significant for TNF-α (<it>p </it>≤ 0.05) but not for IL-6.</p> <p>Conclusion</p> <p>In conclusion, HESA-A was effective against influenza infection through suppressing cytokine expression.</p> |
| first_indexed | 2024-12-18T13:59:17Z |
| format | Article |
| id | doaj.art-65cec0f3f78f45cfb71dfbc7567bc870 |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-12-18T13:59:17Z |
| publishDate | 2012-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-65cec0f3f78f45cfb71dfbc7567bc8702022-12-21T21:05:21ZengBMCVirology Journal1743-422X2012-02-01914410.1186/1743-422X-9-44Attenuation of influenza virus infectivity with herbal-marine compound (HESA-A): an in vitro study in MDCK cellsMehrbod ParvanehIderis AiniOmar Abdul RahmanHair-Bejo MohdTan Sheau WeiKheiri MasoumehTabatabaian Mansoureh<p>Abstract</p> <p>Background</p> <p>The influenza virus is still one of the most important respiratory risks affecting humans which require effective treatments. In this case, traditional medications are of interest. HESA-A is an active natural biological compound from herbal-marine origin. Previous studies have reported that the therapeutic properties of HESA-A are able to treat psoriasis vulgaris and cancers. However, no antiviral properties have been reported.</p> <p>Methods</p> <p>This study was designed to investigate the potential antiviral properties of HESA-A and its effects in modulating TNF-α and IL-6 cytokine levels. HESA-A was prepared in normal saline as a stock solution (0.8 mg/ml, pH = 7.4). Percentages of cell survival when exposed to different concentrations of HESA-A at different time intervals was determined by MTT assay. To study the potential antiviral activity of HESA-A, Madin-Darby Canine Kidney (MDCK) cells were treated with the effective concentration (EC<sub>50</sub>) of HESA-A (0.025 mg/ml) and 100 TCID<sub>50</sub>/0.1 ml of virus sample under different types of exposure.</p> <p>Results</p> <p>Based on the MTT method and hemagglutination assay (HA), HESA-A is capable of improving cell viability to 31% and decreasing HA titre to almost 99% in co-penetration exposures. In addition, based on quantitative real-time PCR (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA), it was found that HESA-A causes decrements in TNF-α and IL-6 cytokine expressions, which was significant for TNF-α (<it>p </it>≤ 0.05) but not for IL-6.</p> <p>Conclusion</p> <p>In conclusion, HESA-A was effective against influenza infection through suppressing cytokine expression.</p>http://www.virologyj.com/content/9/1/44HESA-AH1N1Influenza virusCytokineTNF-αIL-6 |
| spellingShingle | Mehrbod Parvaneh Ideris Aini Omar Abdul Rahman Hair-Bejo Mohd Tan Sheau Wei Kheiri Masoumeh Tabatabaian Mansoureh Attenuation of influenza virus infectivity with herbal-marine compound (HESA-A): an in vitro study in MDCK cells Virology Journal HESA-A H1N1 Influenza virus Cytokine TNF-α IL-6 |
| title | Attenuation of influenza virus infectivity with herbal-marine compound (HESA-A): an in vitro study in MDCK cells |
| title_full | Attenuation of influenza virus infectivity with herbal-marine compound (HESA-A): an in vitro study in MDCK cells |
| title_fullStr | Attenuation of influenza virus infectivity with herbal-marine compound (HESA-A): an in vitro study in MDCK cells |
| title_full_unstemmed | Attenuation of influenza virus infectivity with herbal-marine compound (HESA-A): an in vitro study in MDCK cells |
| title_short | Attenuation of influenza virus infectivity with herbal-marine compound (HESA-A): an in vitro study in MDCK cells |
| title_sort | attenuation of influenza virus infectivity with herbal marine compound hesa a an in vitro study in mdck cells |
| topic | HESA-A H1N1 Influenza virus Cytokine TNF-α IL-6 |
| url | http://www.virologyj.com/content/9/1/44 |
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