Bacterial Microflora of Roach (Rutilus rutilus) Caught in the Driksna River in Latvia
Fish and fish products are a food group with a high nutritional value, however, the fish may contains a variety of microorganisms, which may cause foodborne infection outbreaks in humans after consumption. The aim of the present study was to identify the bacterial microflora of skin, gill and gut of...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Agroprint Timisoara
2023-09-01
|
| Series: | Scientific Papers Animal Science and Biotechnologies |
| Subjects: | |
| Online Access: | https://spasb.ro/index.php/public_html/article/view/1125 |
| _version_ | 1797215424957382656 |
|---|---|
| author | Alīna Klūga Miroslava Kačániová Attila Kántor Margarita Terentjeva |
| author_facet | Alīna Klūga Miroslava Kačániová Attila Kántor Margarita Terentjeva |
| author_sort | Alīna Klūga |
| collection | DOAJ |
| description | Fish and fish products are a food group with a high nutritional value, however, the fish may contains a variety of microorganisms, which may cause foodborne infection outbreaks in humans after consumption. The aim of the present study was to identify the bacterial microflora of skin, gill and gut of roach caught in river. Altogether, nine roach (Rutilus rutilus) samples were collected from fishermen from Driksna river in Latvia. Skin, gills and gut samples were investigated for total bacterial count (TBC), coliforms and Enterobacteriaceae. Pooled skin, muscles and internal organ samples were examined for presence of pathogens – Salmonella spp., Listeria spp. and Yersinia spp. in accordance with ISO standards. Identification of bacterial species was performed with MALDI-TOF MS Biotyper. TBC ranged from 1.96 to 5.16 log10 CFU g-1, coliforms from 0 to 3.66 log10 CFU g-1 and Enterobacteriaceae from 0 to 4.16 log10 CFU g-1 in roach skin, gills and gut samples. The highest counts of TBC, coliforms and Enterobacteriaceae was found in roach gills and gut samples, while there were no significant differences between the TBC in skin, gills and gut (p<0.05). Rahnella spp. and Pseudomonas spp. were the dominant bacteria identified in fish skin, gills and gut. These microorganisms promote the development of deterioration processes of fish meat, and also can be associated with human infections. Salmonella spp., Listeria spp. and Yersinia spp. were not detected in fish samples, however, the presence of potential human pathogens - Aeromonas spp. and Staphylococcus spp. in roach samples was observed. |
| first_indexed | 2024-04-24T11:29:51Z |
| format | Article |
| id | doaj.art-65cf58ced1db4271bd34cd4d4177d982 |
| institution | Directory Open Access Journal |
| issn | 1841-9364 2344-4576 |
| language | English |
| last_indexed | 2024-04-24T11:29:51Z |
| publishDate | 2023-09-01 |
| publisher | Agroprint Timisoara |
| record_format | Article |
| series | Scientific Papers Animal Science and Biotechnologies |
| spelling | doaj.art-65cf58ced1db4271bd34cd4d4177d9822024-04-10T10:38:35ZengAgroprint TimisoaraScientific Papers Animal Science and Biotechnologies1841-93642344-45762023-09-015012592591125Bacterial Microflora of Roach (Rutilus rutilus) Caught in the Driksna River in LatviaAlīna Klūga0Miroslava Kačániová1Attila Kántor2Margarita Terentjeva3Latvia University of Agriculture, Faculty of Veterinary Medicine, Institute of Food and Environmental Hygiene, K. Helmaņa iela 8, Jelgava, LV-3004,LatviaSlovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Nitra 949 76, Tr. A. Hlinku 2, SlovakiaSlovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Nitra 949 76, Tr. A. Hlinku 2, SlovakiaLatvia University of Agriculture, Faculty of Veterinary Medicine, Institute of Food and Environmental Hygiene, K. Helmaņa iela 8, Jelgava, LV-3004,LatviaFish and fish products are a food group with a high nutritional value, however, the fish may contains a variety of microorganisms, which may cause foodborne infection outbreaks in humans after consumption. The aim of the present study was to identify the bacterial microflora of skin, gill and gut of roach caught in river. Altogether, nine roach (Rutilus rutilus) samples were collected from fishermen from Driksna river in Latvia. Skin, gills and gut samples were investigated for total bacterial count (TBC), coliforms and Enterobacteriaceae. Pooled skin, muscles and internal organ samples were examined for presence of pathogens – Salmonella spp., Listeria spp. and Yersinia spp. in accordance with ISO standards. Identification of bacterial species was performed with MALDI-TOF MS Biotyper. TBC ranged from 1.96 to 5.16 log10 CFU g-1, coliforms from 0 to 3.66 log10 CFU g-1 and Enterobacteriaceae from 0 to 4.16 log10 CFU g-1 in roach skin, gills and gut samples. The highest counts of TBC, coliforms and Enterobacteriaceae was found in roach gills and gut samples, while there were no significant differences between the TBC in skin, gills and gut (p<0.05). Rahnella spp. and Pseudomonas spp. were the dominant bacteria identified in fish skin, gills and gut. These microorganisms promote the development of deterioration processes of fish meat, and also can be associated with human infections. Salmonella spp., Listeria spp. and Yersinia spp. were not detected in fish samples, however, the presence of potential human pathogens - Aeromonas spp. and Staphylococcus spp. in roach samples was observed.https://spasb.ro/index.php/public_html/article/view/1125freshwater fishroachbacteriamicrofloramaldi-tof ms biotyper |
| spellingShingle | Alīna Klūga Miroslava Kačániová Attila Kántor Margarita Terentjeva Bacterial Microflora of Roach (Rutilus rutilus) Caught in the Driksna River in Latvia Scientific Papers Animal Science and Biotechnologies freshwater fish roach bacteria microflora maldi-tof ms biotyper |
| title | Bacterial Microflora of Roach (Rutilus rutilus) Caught in the Driksna River in Latvia |
| title_full | Bacterial Microflora of Roach (Rutilus rutilus) Caught in the Driksna River in Latvia |
| title_fullStr | Bacterial Microflora of Roach (Rutilus rutilus) Caught in the Driksna River in Latvia |
| title_full_unstemmed | Bacterial Microflora of Roach (Rutilus rutilus) Caught in the Driksna River in Latvia |
| title_short | Bacterial Microflora of Roach (Rutilus rutilus) Caught in the Driksna River in Latvia |
| title_sort | bacterial microflora of roach rutilus rutilus caught in the driksna river in latvia |
| topic | freshwater fish roach bacteria microflora maldi-tof ms biotyper |
| url | https://spasb.ro/index.php/public_html/article/view/1125 |
| work_keys_str_mv | AT alinakluga bacterialmicrofloraofroachrutilusrutiluscaughtinthedriksnariverinlatvia AT miroslavakacaniova bacterialmicrofloraofroachrutilusrutiluscaughtinthedriksnariverinlatvia AT attilakantor bacterialmicrofloraofroachrutilusrutiluscaughtinthedriksnariverinlatvia AT margaritaterentjeva bacterialmicrofloraofroachrutilusrutiluscaughtinthedriksnariverinlatvia |