Determination of Antibiotic and Heavy Metal Resistance in Paenibacillus lautus 51ATA
Microbial and chemical contamination is one of the biggest environmental problems since exposure to pathogenic microorganisms, such as food poisoning bacteria and their biological by-products, are often associated with disease and allergic reactions. Paenibacillus lautus is an aerobic or facultative...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Turkish Science and Technology Publishing (TURSTEP)
2019-09-01
|
| Series: | Turkish Journal of Agriculture: Food Science and Technology |
| Subjects: | |
| Online Access: | http://www.agrifoodscience.com/index.php/TURJAF/article/view/2824 |
| _version_ | 1797908960894779392 |
|---|---|
| author | Elif Canpolat Burcu Biterge-Süt |
| author_facet | Elif Canpolat Burcu Biterge-Süt |
| author_sort | Elif Canpolat |
| collection | DOAJ |
| description | Microbial and chemical contamination is one of the biggest environmental problems since exposure to pathogenic microorganisms, such as food poisoning bacteria and their biological by-products, are often associated with disease and allergic reactions. Paenibacillus lautus is an aerobic or facultative anaerobic opportunistic bacterial pathogen, which is found in a variety of sources. In this study, we isolated and characterized P. lautus from Niğde Province, which is a wastewater disposal area and therefore presents a great source of contamination. The bacterial isolate was grown, colony morphology and Gram-reactions were observed. Bacteria were identified by 16S rDNA sequencing analysis, which was performed using DNA samples isolated from pure cultures and amplified by polymerase chain reaction (PCR). Sequencing results were compared against known bacterial strains using BLAST (Basic Local Alignment Search Tool) across databases. Antibiotic resistance against 20 different antibiotics and heavy metal resistance against different concentrations of zinc, copper, iron, cobalt, chromium and silver containing compounds were tested via disk diffusion method. Inhibition zones were examined and minimum inhibitory concentrations were recorded. Our results indicated that P. lautus was resistant to a certain set of antibiotics, while it was sensitive against others. Similarly, the bacteria were resistant against high concentrations of most metals tested. In summary, this study provided the first data regarding the isolation and characterization of Paenibacillus lautus strain from a local source in Turkey, which will account as preliminary data and guide our future efforts to fight against microbial contaminations. |
| first_indexed | 2024-04-10T11:00:57Z |
| format | Article |
| id | doaj.art-65cf59c2d14a47cabb5987fb4b7ebbac |
| institution | Directory Open Access Journal |
| issn | 2148-127X |
| language | English |
| last_indexed | 2024-04-10T11:00:57Z |
| publishDate | 2019-09-01 |
| publisher | Turkish Science and Technology Publishing (TURSTEP) |
| record_format | Article |
| series | Turkish Journal of Agriculture: Food Science and Technology |
| spelling | doaj.art-65cf59c2d14a47cabb5987fb4b7ebbac2023-02-15T16:19:42ZengTurkish Science and Technology Publishing (TURSTEP)Turkish Journal of Agriculture: Food Science and Technology2148-127X2019-09-01791465146810.24925/turjaf.v7i8.%p.28241250Determination of Antibiotic and Heavy Metal Resistance in Paenibacillus lautus 51ATAElif Canpolat0Burcu Biterge-Süt1Department of Plant Production and Technologies, Ayhan Şahenk Faculty of Agricultural Sciences and Technologies, Niğde Ömer Halisdemir University, 51240 Merkez/NiğdeDepartment of Medical Biology, Faculty of Medicine, Niğde Ömer Halisdemir University, 51240 Merkez/Microbial and chemical contamination is one of the biggest environmental problems since exposure to pathogenic microorganisms, such as food poisoning bacteria and their biological by-products, are often associated with disease and allergic reactions. Paenibacillus lautus is an aerobic or facultative anaerobic opportunistic bacterial pathogen, which is found in a variety of sources. In this study, we isolated and characterized P. lautus from Niğde Province, which is a wastewater disposal area and therefore presents a great source of contamination. The bacterial isolate was grown, colony morphology and Gram-reactions were observed. Bacteria were identified by 16S rDNA sequencing analysis, which was performed using DNA samples isolated from pure cultures and amplified by polymerase chain reaction (PCR). Sequencing results were compared against known bacterial strains using BLAST (Basic Local Alignment Search Tool) across databases. Antibiotic resistance against 20 different antibiotics and heavy metal resistance against different concentrations of zinc, copper, iron, cobalt, chromium and silver containing compounds were tested via disk diffusion method. Inhibition zones were examined and minimum inhibitory concentrations were recorded. Our results indicated that P. lautus was resistant to a certain set of antibiotics, while it was sensitive against others. Similarly, the bacteria were resistant against high concentrations of most metals tested. In summary, this study provided the first data regarding the isolation and characterization of Paenibacillus lautus strain from a local source in Turkey, which will account as preliminary data and guide our future efforts to fight against microbial contaminations.http://www.agrifoodscience.com/index.php/TURJAF/article/view/2824paenibacillus lautusantibiotic resistanceheavy metal resistanceenvironmental isolatespublic health |
| spellingShingle | Elif Canpolat Burcu Biterge-Süt Determination of Antibiotic and Heavy Metal Resistance in Paenibacillus lautus 51ATA Turkish Journal of Agriculture: Food Science and Technology paenibacillus lautus antibiotic resistance heavy metal resistance environmental isolates public health |
| title | Determination of Antibiotic and Heavy Metal Resistance in Paenibacillus lautus 51ATA |
| title_full | Determination of Antibiotic and Heavy Metal Resistance in Paenibacillus lautus 51ATA |
| title_fullStr | Determination of Antibiotic and Heavy Metal Resistance in Paenibacillus lautus 51ATA |
| title_full_unstemmed | Determination of Antibiotic and Heavy Metal Resistance in Paenibacillus lautus 51ATA |
| title_short | Determination of Antibiotic and Heavy Metal Resistance in Paenibacillus lautus 51ATA |
| title_sort | determination of antibiotic and heavy metal resistance in paenibacillus lautus 51ata |
| topic | paenibacillus lautus antibiotic resistance heavy metal resistance environmental isolates public health |
| url | http://www.agrifoodscience.com/index.php/TURJAF/article/view/2824 |
| work_keys_str_mv | AT elifcanpolat determinationofantibioticandheavymetalresistanceinpaenibacilluslautus51ata AT burcubitergesut determinationofantibioticandheavymetalresistanceinpaenibacilluslautus51ata |