Morphologic Changes of Mammary Carcinomas in Mice over Time as Monitored by Flat-Panel Detector Volume Computed Tomography
Noninvasive methods are strongly needed to detect and quantify not only tumor growth in murine tumor models but also the development of vascularization and necrosis within tumors. This study investigates the use of a new imaging technique, flat-panel detector volume computed tomography (fpVCT), to m...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2008-07-01
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| Series: | Neoplasia: An International Journal for Oncology Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1476558608800048 |
| _version_ | 1819044435800883200 |
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| author | Jeannine Missbach-Guentner Christian Dullin Sarah Kimmina Marta Zientkowska Melanie Domeyer-Missbach Cordula Malz Eckhardt Grabbe Walter Stühmer Frauke Alves |
| author_facet | Jeannine Missbach-Guentner Christian Dullin Sarah Kimmina Marta Zientkowska Melanie Domeyer-Missbach Cordula Malz Eckhardt Grabbe Walter Stühmer Frauke Alves |
| author_sort | Jeannine Missbach-Guentner |
| collection | DOAJ |
| description | Noninvasive methods are strongly needed to detect and quantify not only tumor growth in murine tumor models but also the development of vascularization and necrosis within tumors. This study investigates the use of a new imaging technique, flat-panel detector volume computed tomography (fpVCT), to monitor in vivo tumor progression and structural changes within tumors of two murine carcinoma models. After tumor cell inoculation, single fpVCT scans of the entire mice were performed at different time points. The acquired isotropic, high-resolution volume data sets enable an accurate real-time assessment and precise measurements of tumor volumes. Spreading of contrast agent-containing blood vessels around and within the tumors was clearly visible over time. Furthermore, fpVCT permits the identification of differences in the uptake of contrast media within tumors, thus delineating necrosis, tumor tissues, and blood vessels. Classification of tumor tissues based on the decomposition of the underlying mixture distribution of tissue-related Hounsfield units allowed the quantitative acquisition of necrotic tissues at each time point. Morphologic alterations of the tumor depicted by fpVCT were confirmed by histopathologic examination. Concluding, our data show that fpVCT may be highly suitable for the noninvasive evaluation of tumor responses to anticancer therapies during the course of the disease. |
| first_indexed | 2024-12-21T10:12:38Z |
| format | Article |
| id | doaj.art-65d184424cee42b68c9d8ed4fae4da98 |
| institution | Directory Open Access Journal |
| issn | 1476-5586 1522-8002 |
| language | English |
| last_indexed | 2024-12-21T10:12:38Z |
| publishDate | 2008-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Neoplasia: An International Journal for Oncology Research |
| spelling | doaj.art-65d184424cee42b68c9d8ed4fae4da982022-12-21T19:07:40ZengElsevierNeoplasia: An International Journal for Oncology Research1476-55861522-80022008-07-0110766367310.1593/neo.08270Morphologic Changes of Mammary Carcinomas in Mice over Time as Monitored by Flat-Panel Detector Volume Computed TomographyJeannine Missbach-Guentner0Christian Dullin1Sarah Kimmina2Marta Zientkowska3Melanie Domeyer-Missbach4Cordula Malz5Eckhardt Grabbe6Walter Stühmer7Frauke Alves8Department of Hematology and Oncology, University Medicine, Robert-Koch-Str. 40, 37075 Göttingen, GermanyDepartment of Diagnostic Radiology, University Medicine, Robert-Koch-Str. 40, 37075 Göttingen, GermanyDepartment of Laboratory Animal Science, University Medicine, Robert-Koch-Str. 40, 37075 Göttingen, GermanyDepartment of Hematology and Oncology, University Medicine, Robert-Koch-Str. 40, 37075 Göttingen, GermanyDepartment of Hematology and Oncology, University Medicine, Robert-Koch-Str. 40, 37075 Göttingen, GermanyDepartment of Anatomy and Embryology, University Medicine, Robert-Koch-Str. 40, 37075 Göttingen, GermanyDepartment of Diagnostic Radiology, University Medicine, Robert-Koch-Str. 40, 37075 Göttingen, GermanyMax-Planck-Institute of Experimental Medicine, Hermann Rein Str. 3, 37075 Göttingen, GermanyDepartment of Hematology and Oncology, University Medicine, Robert-Koch-Str. 40, 37075 Göttingen, GermanyNoninvasive methods are strongly needed to detect and quantify not only tumor growth in murine tumor models but also the development of vascularization and necrosis within tumors. This study investigates the use of a new imaging technique, flat-panel detector volume computed tomography (fpVCT), to monitor in vivo tumor progression and structural changes within tumors of two murine carcinoma models. After tumor cell inoculation, single fpVCT scans of the entire mice were performed at different time points. The acquired isotropic, high-resolution volume data sets enable an accurate real-time assessment and precise measurements of tumor volumes. Spreading of contrast agent-containing blood vessels around and within the tumors was clearly visible over time. Furthermore, fpVCT permits the identification of differences in the uptake of contrast media within tumors, thus delineating necrosis, tumor tissues, and blood vessels. Classification of tumor tissues based on the decomposition of the underlying mixture distribution of tissue-related Hounsfield units allowed the quantitative acquisition of necrotic tissues at each time point. Morphologic alterations of the tumor depicted by fpVCT were confirmed by histopathologic examination. Concluding, our data show that fpVCT may be highly suitable for the noninvasive evaluation of tumor responses to anticancer therapies during the course of the disease.http://www.sciencedirect.com/science/article/pii/S1476558608800048 |
| spellingShingle | Jeannine Missbach-Guentner Christian Dullin Sarah Kimmina Marta Zientkowska Melanie Domeyer-Missbach Cordula Malz Eckhardt Grabbe Walter Stühmer Frauke Alves Morphologic Changes of Mammary Carcinomas in Mice over Time as Monitored by Flat-Panel Detector Volume Computed Tomography Neoplasia: An International Journal for Oncology Research |
| title | Morphologic Changes of Mammary Carcinomas in Mice over Time as Monitored by Flat-Panel Detector Volume Computed Tomography |
| title_full | Morphologic Changes of Mammary Carcinomas in Mice over Time as Monitored by Flat-Panel Detector Volume Computed Tomography |
| title_fullStr | Morphologic Changes of Mammary Carcinomas in Mice over Time as Monitored by Flat-Panel Detector Volume Computed Tomography |
| title_full_unstemmed | Morphologic Changes of Mammary Carcinomas in Mice over Time as Monitored by Flat-Panel Detector Volume Computed Tomography |
| title_short | Morphologic Changes of Mammary Carcinomas in Mice over Time as Monitored by Flat-Panel Detector Volume Computed Tomography |
| title_sort | morphologic changes of mammary carcinomas in mice over time as monitored by flat panel detector volume computed tomography |
| url | http://www.sciencedirect.com/science/article/pii/S1476558608800048 |
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