Sulfonated Starch-<i>Graft</i>-Polyaniline@Graphene Electrically Conductive Nanocomposite: Application for Tyrosinase Immobilization
The interaction of tyrosinase with sulfonated starch-<i>graft</i>-polyaniline@graphene (SSt-<i>g</i>-PANI@G) nanocomposite was investigated by electrochemical methods. The activity of the immobilized tyrosinase (Tyase) was proved by the electrochemical detection of three subs...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-10-01
|
| Series: | Biosensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6374/12/11/939 |
| _version_ | 1797468923989327872 |
|---|---|
| author | Marzieh Aliya Ehsan Nazarzadeh Zare Hassan Faridnouri Matineh Ghomi Pooyan Makvandi |
| author_facet | Marzieh Aliya Ehsan Nazarzadeh Zare Hassan Faridnouri Matineh Ghomi Pooyan Makvandi |
| author_sort | Marzieh Aliya |
| collection | DOAJ |
| description | The interaction of tyrosinase with sulfonated starch-<i>graft</i>-polyaniline@graphene (SSt-<i>g</i>-PANI@G) nanocomposite was investigated by electrochemical methods. The activity of the immobilized tyrosinase (Tyase) was proved by the electrochemical detection of three substrates (L-dopa, caffeic acid, and catechol). The SSt-<i>g</i>-PANI@G nanocomposite was characterized by Fourier-transform infrared spectra (FT-IR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), energy-dispersive X-ray analysis (EDX), and thermogravimetric analysis (TGA). To immobilize tyrosinase on the surface of the nanocomposite, a simple drop-casting technique was used. The presence of sulfuric acid and hydroxyl groups in SSt, amine groups in PANI, and high surface-to-volume ratio and electrical conductivity of graphene in the prepared nanocomposite led to good enzyme immobilization on the electrode surface. The modified electrode showed a suitable catalytic effect on the electrochemical redox agent, compared with the bare electrode. The peak current responses for three substrates were studied with a calibration curve derived using cyclic voltammetry (CV) and differential pulse voltammetry (DPV). In addition, the fabricated SSt-g-PANI@G/Tyase/GCE showed a more suitable response to catechol, L-dopa, and caffeic acid substrates, respectively. |
| first_indexed | 2024-03-09T19:14:09Z |
| format | Article |
| id | doaj.art-65d19a54d4ce485a979429aab6950645 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T19:14:09Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-65d19a54d4ce485a979429aab69506452023-11-24T03:54:42ZengMDPI AGBiosensors2079-63742022-10-01121193910.3390/bios12110939Sulfonated Starch-<i>Graft</i>-Polyaniline@Graphene Electrically Conductive Nanocomposite: Application for Tyrosinase ImmobilizationMarzieh Aliya0Ehsan Nazarzadeh Zare1Hassan Faridnouri2Matineh Ghomi3Pooyan Makvandi4School of Chemistry, Damghan University, Damghan 36716-41167, IranSchool of Chemistry, Damghan University, Damghan 36716-41167, IranSchool of Biology, Damghan University, Damghan 36716-41167, IranSchool of Chemistry, Damghan University, Damghan 36716-41167, IranCentre for Materials Interface, Istituto Italiano di Tecnologia, Viale Rinaldo Piaggio 34, Pontedera, 56025 Pisa, ItalyThe interaction of tyrosinase with sulfonated starch-<i>graft</i>-polyaniline@graphene (SSt-<i>g</i>-PANI@G) nanocomposite was investigated by electrochemical methods. The activity of the immobilized tyrosinase (Tyase) was proved by the electrochemical detection of three substrates (L-dopa, caffeic acid, and catechol). The SSt-<i>g</i>-PANI@G nanocomposite was characterized by Fourier-transform infrared spectra (FT-IR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), energy-dispersive X-ray analysis (EDX), and thermogravimetric analysis (TGA). To immobilize tyrosinase on the surface of the nanocomposite, a simple drop-casting technique was used. The presence of sulfuric acid and hydroxyl groups in SSt, amine groups in PANI, and high surface-to-volume ratio and electrical conductivity of graphene in the prepared nanocomposite led to good enzyme immobilization on the electrode surface. The modified electrode showed a suitable catalytic effect on the electrochemical redox agent, compared with the bare electrode. The peak current responses for three substrates were studied with a calibration curve derived using cyclic voltammetry (CV) and differential pulse voltammetry (DPV). In addition, the fabricated SSt-g-PANI@G/Tyase/GCE showed a more suitable response to catechol, L-dopa, and caffeic acid substrates, respectively.https://www.mdpi.com/2079-6374/12/11/939sulfonated starchpolyanilinegrapheneelectrical nanocompositetyrosinase |
| spellingShingle | Marzieh Aliya Ehsan Nazarzadeh Zare Hassan Faridnouri Matineh Ghomi Pooyan Makvandi Sulfonated Starch-<i>Graft</i>-Polyaniline@Graphene Electrically Conductive Nanocomposite: Application for Tyrosinase Immobilization Biosensors sulfonated starch polyaniline graphene electrical nanocomposite tyrosinase |
| title | Sulfonated Starch-<i>Graft</i>-Polyaniline@Graphene Electrically Conductive Nanocomposite: Application for Tyrosinase Immobilization |
| title_full | Sulfonated Starch-<i>Graft</i>-Polyaniline@Graphene Electrically Conductive Nanocomposite: Application for Tyrosinase Immobilization |
| title_fullStr | Sulfonated Starch-<i>Graft</i>-Polyaniline@Graphene Electrically Conductive Nanocomposite: Application for Tyrosinase Immobilization |
| title_full_unstemmed | Sulfonated Starch-<i>Graft</i>-Polyaniline@Graphene Electrically Conductive Nanocomposite: Application for Tyrosinase Immobilization |
| title_short | Sulfonated Starch-<i>Graft</i>-Polyaniline@Graphene Electrically Conductive Nanocomposite: Application for Tyrosinase Immobilization |
| title_sort | sulfonated starch i graft i polyaniline graphene electrically conductive nanocomposite application for tyrosinase immobilization |
| topic | sulfonated starch polyaniline graphene electrical nanocomposite tyrosinase |
| url | https://www.mdpi.com/2079-6374/12/11/939 |
| work_keys_str_mv | AT marziehaliya sulfonatedstarchigraftipolyanilinegrapheneelectricallyconductivenanocompositeapplicationfortyrosinaseimmobilization AT ehsannazarzadehzare sulfonatedstarchigraftipolyanilinegrapheneelectricallyconductivenanocompositeapplicationfortyrosinaseimmobilization AT hassanfaridnouri sulfonatedstarchigraftipolyanilinegrapheneelectricallyconductivenanocompositeapplicationfortyrosinaseimmobilization AT matinehghomi sulfonatedstarchigraftipolyanilinegrapheneelectricallyconductivenanocompositeapplicationfortyrosinaseimmobilization AT pooyanmakvandi sulfonatedstarchigraftipolyanilinegrapheneelectricallyconductivenanocompositeapplicationfortyrosinaseimmobilization |