Molecular characterization of marine bacterial isolates of Visakhapatnam coast—efficacy in dye decolorization and bioremediation of cadmium
Abstract Background Microbial community is one of the diversified communities of the marine environment. Studies have shown that microorganisms isolated from the marine environment are metabolically active and have adapted to life in the ocean. The marine microorganisms use various survival strategi...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-06-01
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| Series: | Journal of Genetic Engineering and Biotechnology |
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| Online Access: | https://doi.org/10.1186/s43141-021-00189-0 |
| _version_ | 1797203981874757632 |
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| author | Teja Mandragutti Muni Kumar Dokka Bindiya Panchagnula Sudhakar Godi |
| author_facet | Teja Mandragutti Muni Kumar Dokka Bindiya Panchagnula Sudhakar Godi |
| author_sort | Teja Mandragutti |
| collection | DOAJ |
| description | Abstract Background Microbial community is one of the diversified communities of the marine environment. Studies have shown that microorganisms isolated from the marine environment are metabolically active and have adapted to life in the ocean. The marine microorganisms use various survival strategies to combat heavy metal stress and decolorization of various textile dyes, thus playing an important role in the bioremediation of cadmium and degradation of textile dyes. The present study deals with the isolation and 16S rRNA molecular characterization of M3 and M8 bacterial strains isolated from marine water samples collected from Visakhapatnam harbor. M3 and M8 isolates were also checked for their efficacy in the removal of cadmium and decolorization of various textile dyes from the environment. Results The water sample was subjected to tube dilution method to isolate bacterial strains, and ten different isolates were screened. The biochemical tests were performed for the isolates to prove their validity and 16S rRNA molecular sequencing and phylogenetic analysis for species identification. Out of interest, two bacterial strains, namely, M3 and M8 were subjected to 16S rRNA molecular sequencing and phylogenetic analysis and were identified as Bacillus subtilis and Pseudomonas resinovorans. The two bacterial strains showed promising dye degradation property when checked with nine different textile dyes of wavelength ranging from 400 to 600 nm and removal of cadmium from the growth medium. Conclusion The present study demonstrates the isolates M3 and M8 to be potential strains having dye decolorization and bioremediation of cadmium applications. |
| first_indexed | 2024-04-24T08:27:58Z |
| format | Article |
| id | doaj.art-65d3424ab84a4ea08e63289941f93e5a |
| institution | Directory Open Access Journal |
| issn | 2090-5920 |
| language | English |
| last_indexed | 2024-04-24T08:27:58Z |
| publishDate | 2021-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Genetic Engineering and Biotechnology |
| spelling | doaj.art-65d3424ab84a4ea08e63289941f93e5a2024-04-16T21:22:10ZengElsevierJournal of Genetic Engineering and Biotechnology2090-59202021-06-0119111110.1186/s43141-021-00189-0Molecular characterization of marine bacterial isolates of Visakhapatnam coast—efficacy in dye decolorization and bioremediation of cadmiumTeja Mandragutti0Muni Kumar Dokka1Bindiya Panchagnula2Sudhakar Godi3Department of Biotechnology, Andhra UniversityDepartment of Biochemistry, Andhra UniversityDepartment of Biotechnology, Andhra UniversityDepartment of Human Genetics, Andhra UniversityAbstract Background Microbial community is one of the diversified communities of the marine environment. Studies have shown that microorganisms isolated from the marine environment are metabolically active and have adapted to life in the ocean. The marine microorganisms use various survival strategies to combat heavy metal stress and decolorization of various textile dyes, thus playing an important role in the bioremediation of cadmium and degradation of textile dyes. The present study deals with the isolation and 16S rRNA molecular characterization of M3 and M8 bacterial strains isolated from marine water samples collected from Visakhapatnam harbor. M3 and M8 isolates were also checked for their efficacy in the removal of cadmium and decolorization of various textile dyes from the environment. Results The water sample was subjected to tube dilution method to isolate bacterial strains, and ten different isolates were screened. The biochemical tests were performed for the isolates to prove their validity and 16S rRNA molecular sequencing and phylogenetic analysis for species identification. Out of interest, two bacterial strains, namely, M3 and M8 were subjected to 16S rRNA molecular sequencing and phylogenetic analysis and were identified as Bacillus subtilis and Pseudomonas resinovorans. The two bacterial strains showed promising dye degradation property when checked with nine different textile dyes of wavelength ranging from 400 to 600 nm and removal of cadmium from the growth medium. Conclusion The present study demonstrates the isolates M3 and M8 to be potential strains having dye decolorization and bioremediation of cadmium applications.https://doi.org/10.1186/s43141-021-00189-0Bacillus subtilisPseudomonas resinovorans16S rRNAPhylogenetic analysisBioremediation |
| spellingShingle | Teja Mandragutti Muni Kumar Dokka Bindiya Panchagnula Sudhakar Godi Molecular characterization of marine bacterial isolates of Visakhapatnam coast—efficacy in dye decolorization and bioremediation of cadmium Journal of Genetic Engineering and Biotechnology Bacillus subtilis Pseudomonas resinovorans 16S rRNA Phylogenetic analysis Bioremediation |
| title | Molecular characterization of marine bacterial isolates of Visakhapatnam coast—efficacy in dye decolorization and bioremediation of cadmium |
| title_full | Molecular characterization of marine bacterial isolates of Visakhapatnam coast—efficacy in dye decolorization and bioremediation of cadmium |
| title_fullStr | Molecular characterization of marine bacterial isolates of Visakhapatnam coast—efficacy in dye decolorization and bioremediation of cadmium |
| title_full_unstemmed | Molecular characterization of marine bacterial isolates of Visakhapatnam coast—efficacy in dye decolorization and bioremediation of cadmium |
| title_short | Molecular characterization of marine bacterial isolates of Visakhapatnam coast—efficacy in dye decolorization and bioremediation of cadmium |
| title_sort | molecular characterization of marine bacterial isolates of visakhapatnam coast efficacy in dye decolorization and bioremediation of cadmium |
| topic | Bacillus subtilis Pseudomonas resinovorans 16S rRNA Phylogenetic analysis Bioremediation |
| url | https://doi.org/10.1186/s43141-021-00189-0 |
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