The retinoid metabolism of Gammarus fossarum is disrupted by exogenous all-trans retinoic acid, citral, and methoprene but not by the technical formulation of glyphosate
Over the last decade, fluctuations of retinoids (RETs), also known as vitamin A and derivatives, have proved to be useful biomarkers to assess the environmental chemical pressure on a wide variety of non-target vertebrates. This use of RET-based biomarkers is of particular interest in the non-target...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-03-01
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| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651323001069 |
| _version_ | 1797903031571841024 |
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| author | Maxime Gauthier Gaëlle Daniele Barbara Giroud Florent Lafay Emmanuelle Vulliet Catherine Jumarie Jeanne Garric Monique Boily Olivier Geffard |
| author_facet | Maxime Gauthier Gaëlle Daniele Barbara Giroud Florent Lafay Emmanuelle Vulliet Catherine Jumarie Jeanne Garric Monique Boily Olivier Geffard |
| author_sort | Maxime Gauthier |
| collection | DOAJ |
| description | Over the last decade, fluctuations of retinoids (RETs), also known as vitamin A and derivatives, have proved to be useful biomarkers to assess the environmental chemical pressure on a wide variety of non-target vertebrates. This use of RET-based biomarkers is of particular interest in the non-target sentinel species Gammarus fossarum in which RETs were shown to influence crucial physiological functions. To study and probe this metabolism in this crustacean model, a UHPLC-MS/MS method was developed to 1) identify and 2) monitor several endogenous RETs in unexposed females throughout their reproductive cycle. Then, females were exposed in controlled conditions to exogenous all-trans retinoic acid (atRA) and citral (CIT), a RA synthesis inhibitor, to simulate an excess or deficiency in RA. Perturbation of vitamin A metabolism by pesticides was further studied in response to methoprene (MET), a juvenile hormone analog as well as glyphosate (GLY). The developed method allowed, for the first time in this model, the identification of RA metabolites (all-trans 4-oxo and 13-cis 4-oxo RA), RA isomers (all-trans and 13-cis RA) as well as retinaldehyde (RALD) isomers (all-trans, 11-cis, and 13-cis RALD) and showed two distinct phases in the reproductive cycle. Retinoic acid successfully increased the tissular concentration of both RA isomers and CIT proved to be efficient at perturbating the conversion from RALD to RA. Methoprene perturbed the ratios between RA isomers whereas GLY had no observed effects on the RET system of G. fossarum females. We were able to discriminate different dynamics of RET perturbations by morphogens (atRA or CIT) or MET which highlights the plausible mediation of RETs in MET-induced disorders. Ultimately, our study shows that RETs are influenced by exposure to MET and strengthen their potential to assess aquatic ecosystem chemical status. |
| first_indexed | 2024-04-10T09:25:27Z |
| format | Article |
| id | doaj.art-65d7b3f1b5944522a726bef6baeb539e |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-04-10T09:25:27Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-65d7b3f1b5944522a726bef6baeb539e2023-02-20T04:08:39ZengElsevierEcotoxicology and Environmental Safety0147-65132023-03-01252114602The retinoid metabolism of Gammarus fossarum is disrupted by exogenous all-trans retinoic acid, citral, and methoprene but not by the technical formulation of glyphosateMaxime Gauthier0Gaëlle Daniele1Barbara Giroud2Florent Lafay3Emmanuelle Vulliet4Catherine Jumarie5Jeanne Garric6Monique Boily7Olivier Geffard8Unité de recherche RiverLy, INRAE, 5, Rue de la Doua C.S. 20244, Villeurbanne Cedex, France; Université du Québec à Montréal (UQAM), Département des sciences biologiques, 141, Avenue du Président-Kennedy, C.P. 8888, Succursale Centre-Ville, H3C 3P8 Montréal, Québec, CanadaUniv. Lyon, CNRS, Université Claude Bernard Lyon 1, Institut des Sciences Analytiques, UMR 5280, 5, Rue de la Doua, F-69100 Villeurbanne, FranceUniv. Lyon, CNRS, Université Claude Bernard Lyon 1, Institut des Sciences Analytiques, UMR 5280, 5, Rue de la Doua, F-69100 Villeurbanne, FranceUniv. Lyon, CNRS, Université Claude Bernard Lyon 1, Institut des Sciences Analytiques, UMR 5280, 5, Rue de la Doua, F-69100 Villeurbanne, FranceUniv. Lyon, CNRS, Université Claude Bernard Lyon 1, Institut des Sciences Analytiques, UMR 5280, 5, Rue de la Doua, F-69100 Villeurbanne, FranceUniversité du Québec à Montréal (UQAM), Département des sciences biologiques, 141, Avenue du Président-Kennedy, C.P. 8888, Succursale Centre-Ville, H3C 3P8 Montréal, Québec, CanadaUnité de recherche RiverLy, INRAE, 5, Rue de la Doua C.S. 20244, Villeurbanne Cedex, FranceUniversité du Québec à Montréal (UQAM), Département des sciences biologiques, 141, Avenue du Président-Kennedy, C.P. 8888, Succursale Centre-Ville, H3C 3P8 Montréal, Québec, CanadaUnité de recherche RiverLy, INRAE, 5, Rue de la Doua C.S. 20244, Villeurbanne Cedex, France; Correspondence to: INRAE, Research Unit RiverLy, Ecotoxicology Laboratory, 5, Rue de la Doua, C.S. 20244, 69625 Villeurbanne Cedex, France.Over the last decade, fluctuations of retinoids (RETs), also known as vitamin A and derivatives, have proved to be useful biomarkers to assess the environmental chemical pressure on a wide variety of non-target vertebrates. This use of RET-based biomarkers is of particular interest in the non-target sentinel species Gammarus fossarum in which RETs were shown to influence crucial physiological functions. To study and probe this metabolism in this crustacean model, a UHPLC-MS/MS method was developed to 1) identify and 2) monitor several endogenous RETs in unexposed females throughout their reproductive cycle. Then, females were exposed in controlled conditions to exogenous all-trans retinoic acid (atRA) and citral (CIT), a RA synthesis inhibitor, to simulate an excess or deficiency in RA. Perturbation of vitamin A metabolism by pesticides was further studied in response to methoprene (MET), a juvenile hormone analog as well as glyphosate (GLY). The developed method allowed, for the first time in this model, the identification of RA metabolites (all-trans 4-oxo and 13-cis 4-oxo RA), RA isomers (all-trans and 13-cis RA) as well as retinaldehyde (RALD) isomers (all-trans, 11-cis, and 13-cis RALD) and showed two distinct phases in the reproductive cycle. Retinoic acid successfully increased the tissular concentration of both RA isomers and CIT proved to be efficient at perturbating the conversion from RALD to RA. Methoprene perturbed the ratios between RA isomers whereas GLY had no observed effects on the RET system of G. fossarum females. We were able to discriminate different dynamics of RET perturbations by morphogens (atRA or CIT) or MET which highlights the plausible mediation of RETs in MET-induced disorders. Ultimately, our study shows that RETs are influenced by exposure to MET and strengthen their potential to assess aquatic ecosystem chemical status.http://www.sciencedirect.com/science/article/pii/S0147651323001069Retinoic acid isomerizationVitamin AJuvenile hormone agonistsInsecticideAmphipod |
| spellingShingle | Maxime Gauthier Gaëlle Daniele Barbara Giroud Florent Lafay Emmanuelle Vulliet Catherine Jumarie Jeanne Garric Monique Boily Olivier Geffard The retinoid metabolism of Gammarus fossarum is disrupted by exogenous all-trans retinoic acid, citral, and methoprene but not by the technical formulation of glyphosate Ecotoxicology and Environmental Safety Retinoic acid isomerization Vitamin A Juvenile hormone agonists Insecticide Amphipod |
| title | The retinoid metabolism of Gammarus fossarum is disrupted by exogenous all-trans retinoic acid, citral, and methoprene but not by the technical formulation of glyphosate |
| title_full | The retinoid metabolism of Gammarus fossarum is disrupted by exogenous all-trans retinoic acid, citral, and methoprene but not by the technical formulation of glyphosate |
| title_fullStr | The retinoid metabolism of Gammarus fossarum is disrupted by exogenous all-trans retinoic acid, citral, and methoprene but not by the technical formulation of glyphosate |
| title_full_unstemmed | The retinoid metabolism of Gammarus fossarum is disrupted by exogenous all-trans retinoic acid, citral, and methoprene but not by the technical formulation of glyphosate |
| title_short | The retinoid metabolism of Gammarus fossarum is disrupted by exogenous all-trans retinoic acid, citral, and methoprene but not by the technical formulation of glyphosate |
| title_sort | retinoid metabolism of gammarus fossarum is disrupted by exogenous all trans retinoic acid citral and methoprene but not by the technical formulation of glyphosate |
| topic | Retinoic acid isomerization Vitamin A Juvenile hormone agonists Insecticide Amphipod |
| url | http://www.sciencedirect.com/science/article/pii/S0147651323001069 |
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