Multi-subunit BCG booster vaccine GamTBvac: Assessment of immunogenicity and protective efficacy in murine and guinea pig TB models.
New innovative vaccines are highly needed to combat the global threat posed by tuberculosis. Efficient components-antigens and adjuvants-are crucial for development of modern recombinant TB vaccines. This study describes a new vaccine (GamTBvac) consisting of two mycobacterial antigen fusions (Ag85A...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2017-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5409163?pdf=render |
| _version_ | 1817980705089519616 |
|---|---|
| author | A P Tkachuk V A Gushchin V D Potapov A V Demidenko V G Lunin A L Gintsburg |
| author_facet | A P Tkachuk V A Gushchin V D Potapov A V Demidenko V G Lunin A L Gintsburg |
| author_sort | A P Tkachuk |
| collection | DOAJ |
| description | New innovative vaccines are highly needed to combat the global threat posed by tuberculosis. Efficient components-antigens and adjuvants-are crucial for development of modern recombinant TB vaccines. This study describes a new vaccine (GamTBvac) consisting of two mycobacterial antigen fusions (Ag85A and ESAT6-CFP10)-with dextran-binding domain immobilized on dextran and mixed with an adjuvant consisting of DEAE-dextran core, and with CpG oligodeoxynucleotides (TLR9 agonists). GamTBvac and its components were assessed for immunogenicity and protective efficacy in GamTBvac-prime/boost and BCG-prime/ GamTBvac-boost in murine and guinea pig TB models. Results show that in both infectious models, GamTBvac has a strong immunogenicity and significant protective effect against Mycobacterium tuberculosis strain H37Rv under aerosol and intravenous challenges. GamTBvac showed a particularly strong protective effect as a BCG booster vaccine. |
| first_indexed | 2024-04-13T22:56:35Z |
| format | Article |
| id | doaj.art-65dacd46b5f148c48ca2c8469141bc13 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T22:56:35Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-65dacd46b5f148c48ca2c8469141bc132022-12-22T02:25:58ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01124e017678410.1371/journal.pone.0176784Multi-subunit BCG booster vaccine GamTBvac: Assessment of immunogenicity and protective efficacy in murine and guinea pig TB models.A P TkachukV A GushchinV D PotapovA V DemidenkoV G LuninA L GintsburgNew innovative vaccines are highly needed to combat the global threat posed by tuberculosis. Efficient components-antigens and adjuvants-are crucial for development of modern recombinant TB vaccines. This study describes a new vaccine (GamTBvac) consisting of two mycobacterial antigen fusions (Ag85A and ESAT6-CFP10)-with dextran-binding domain immobilized on dextran and mixed with an adjuvant consisting of DEAE-dextran core, and with CpG oligodeoxynucleotides (TLR9 agonists). GamTBvac and its components were assessed for immunogenicity and protective efficacy in GamTBvac-prime/boost and BCG-prime/ GamTBvac-boost in murine and guinea pig TB models. Results show that in both infectious models, GamTBvac has a strong immunogenicity and significant protective effect against Mycobacterium tuberculosis strain H37Rv under aerosol and intravenous challenges. GamTBvac showed a particularly strong protective effect as a BCG booster vaccine.http://europepmc.org/articles/PMC5409163?pdf=render |
| spellingShingle | A P Tkachuk V A Gushchin V D Potapov A V Demidenko V G Lunin A L Gintsburg Multi-subunit BCG booster vaccine GamTBvac: Assessment of immunogenicity and protective efficacy in murine and guinea pig TB models. PLoS ONE |
| title | Multi-subunit BCG booster vaccine GamTBvac: Assessment of immunogenicity and protective efficacy in murine and guinea pig TB models. |
| title_full | Multi-subunit BCG booster vaccine GamTBvac: Assessment of immunogenicity and protective efficacy in murine and guinea pig TB models. |
| title_fullStr | Multi-subunit BCG booster vaccine GamTBvac: Assessment of immunogenicity and protective efficacy in murine and guinea pig TB models. |
| title_full_unstemmed | Multi-subunit BCG booster vaccine GamTBvac: Assessment of immunogenicity and protective efficacy in murine and guinea pig TB models. |
| title_short | Multi-subunit BCG booster vaccine GamTBvac: Assessment of immunogenicity and protective efficacy in murine and guinea pig TB models. |
| title_sort | multi subunit bcg booster vaccine gamtbvac assessment of immunogenicity and protective efficacy in murine and guinea pig tb models |
| url | http://europepmc.org/articles/PMC5409163?pdf=render |
| work_keys_str_mv | AT aptkachuk multisubunitbcgboostervaccinegamtbvacassessmentofimmunogenicityandprotectiveefficacyinmurineandguineapigtbmodels AT vagushchin multisubunitbcgboostervaccinegamtbvacassessmentofimmunogenicityandprotectiveefficacyinmurineandguineapigtbmodels AT vdpotapov multisubunitbcgboostervaccinegamtbvacassessmentofimmunogenicityandprotectiveefficacyinmurineandguineapigtbmodels AT avdemidenko multisubunitbcgboostervaccinegamtbvacassessmentofimmunogenicityandprotectiveefficacyinmurineandguineapigtbmodels AT vglunin multisubunitbcgboostervaccinegamtbvacassessmentofimmunogenicityandprotectiveefficacyinmurineandguineapigtbmodels AT algintsburg multisubunitbcgboostervaccinegamtbvacassessmentofimmunogenicityandprotectiveefficacyinmurineandguineapigtbmodels |