A plasma membrane template for macropinocytic cups
Macropinocytosis is a fundamental mechanism that allows cells to take up extracellular liquid into large vesicles. It critically depends on the formation of a ring of protrusive actin beneath the plasma membrane, which develops into the macropinocytic cup. We show that macropinocytic cups in Dictyos...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2016-12-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/20085 |
| _version_ | 1811181457536712704 |
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| author | Douwe M Veltman Thomas D Williams Gareth Bloomfield Bi-Chang Chen Eric Betzig Robert H Insall Robert R Kay |
| author_facet | Douwe M Veltman Thomas D Williams Gareth Bloomfield Bi-Chang Chen Eric Betzig Robert H Insall Robert R Kay |
| author_sort | Douwe M Veltman |
| collection | DOAJ |
| description | Macropinocytosis is a fundamental mechanism that allows cells to take up extracellular liquid into large vesicles. It critically depends on the formation of a ring of protrusive actin beneath the plasma membrane, which develops into the macropinocytic cup. We show that macropinocytic cups in Dictyostelium are organised around coincident intense patches of PIP3, active Ras and active Rac. These signalling patches are invariably associated with a ring of active SCAR/WAVE at their periphery, as are all examined structures based on PIP3 patches, including phagocytic cups and basal waves. Patch formation does not depend on the enclosing F-actin ring, and patches become enlarged when the RasGAP NF1 is mutated, showing that Ras plays an instructive role. New macropinocytic cups predominantly form by splitting from existing ones. We propose that cup-shaped plasma membrane structures form from self-organizing patches of active Ras/PIP3, which recruit a ring of actin nucleators to their periphery. |
| first_indexed | 2024-04-11T09:18:03Z |
| format | Article |
| id | doaj.art-65db319e912842dca494acd238a2fdf7 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T09:18:03Z |
| publishDate | 2016-12-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-65db319e912842dca494acd238a2fdf72022-12-22T04:32:17ZengeLife Sciences Publications LtdeLife2050-084X2016-12-01510.7554/eLife.20085A plasma membrane template for macropinocytic cupsDouwe M Veltman0https://orcid.org/0000-0002-9512-3235Thomas D Williams1Gareth Bloomfield2Bi-Chang Chen3Eric Betzig4Robert H Insall5Robert R Kay6MRC Laboratory of Molecular Biology, Cambridge, United Kingdom; Beatson Institute for Cancer Research, Glasgow, United KingdomMRC Laboratory of Molecular Biology, Cambridge, United KingdomMRC Laboratory of Molecular Biology, Cambridge, United KingdomJanelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, United StatesJanelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, United StatesBeatson Institute for Cancer Research, Glasgow, United KingdomMRC Laboratory of Molecular Biology, Cambridge, United KingdomMacropinocytosis is a fundamental mechanism that allows cells to take up extracellular liquid into large vesicles. It critically depends on the formation of a ring of protrusive actin beneath the plasma membrane, which develops into the macropinocytic cup. We show that macropinocytic cups in Dictyostelium are organised around coincident intense patches of PIP3, active Ras and active Rac. These signalling patches are invariably associated with a ring of active SCAR/WAVE at their periphery, as are all examined structures based on PIP3 patches, including phagocytic cups and basal waves. Patch formation does not depend on the enclosing F-actin ring, and patches become enlarged when the RasGAP NF1 is mutated, showing that Ras plays an instructive role. New macropinocytic cups predominantly form by splitting from existing ones. We propose that cup-shaped plasma membrane structures form from self-organizing patches of active Ras/PIP3, which recruit a ring of actin nucleators to their periphery.https://elifesciences.org/articles/20085macropinocytosisactin cytoskeletonSCAR/WAVE |
| spellingShingle | Douwe M Veltman Thomas D Williams Gareth Bloomfield Bi-Chang Chen Eric Betzig Robert H Insall Robert R Kay A plasma membrane template for macropinocytic cups eLife macropinocytosis actin cytoskeleton SCAR/WAVE |
| title | A plasma membrane template for macropinocytic cups |
| title_full | A plasma membrane template for macropinocytic cups |
| title_fullStr | A plasma membrane template for macropinocytic cups |
| title_full_unstemmed | A plasma membrane template for macropinocytic cups |
| title_short | A plasma membrane template for macropinocytic cups |
| title_sort | plasma membrane template for macropinocytic cups |
| topic | macropinocytosis actin cytoskeleton SCAR/WAVE |
| url | https://elifesciences.org/articles/20085 |
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