Unravelling the Symbiotic Microalgal Diversity in <i>Buellia zoharyi</i> (Lichenized Ascomycota) from the Iberian Peninsula and Balearic Islands Using DNA Metabarcoding
<i>Buellia zoharyi</i> is a crustose placodioid lichen, usually occurring on biocrusts of semiarid ecosystems in circum-Mediterranean/Macaronesian areas. In previous work, we found that this lichenized fungus was flexible in its phycobiont choice in the Canary Islands. Here we test wheth...
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MDPI AG
2021-05-01
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| Online Access: | https://www.mdpi.com/1424-2818/13/6/220 |
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| author | Patricia Moya Salvador Chiva Arantzazu Molins Isaac Garrido-Benavent Eva Barreno |
| author_facet | Patricia Moya Salvador Chiva Arantzazu Molins Isaac Garrido-Benavent Eva Barreno |
| author_sort | Patricia Moya |
| collection | DOAJ |
| description | <i>Buellia zoharyi</i> is a crustose placodioid lichen, usually occurring on biocrusts of semiarid ecosystems in circum-Mediterranean/Macaronesian areas. In previous work, we found that this lichenized fungus was flexible in its phycobiont choice in the Canary Islands. Here we test whether geography and habitat influence phycobiont diversity in populations of this lichen from the Iberian Peninsula and Balearic Islands using Sanger and high throughput sequencing (HTS). Additionally, three thallus section categories (central, middle and periphery) were analyzed to explore diversity of microalgal communities in each part. We found that <i>B. zoharyi</i> populations hosted at least three different <i>Trebouxia</i> spp., and this lichen can associate with distinct phycobiont strains in different habitats and geographic regions. This study also revealed that the <i>Trebouxia</i> composition of this lichen showed significant differences when comparing the Iberian Peninsula with the Balearics thalli. No support for differences in microalgal communities was found among thallus sections; however, several thalli showed different predominant <i>Trebouxia</i> spp. at each section. This result corroborate that thallus parts selected for DNA extraction in metabarcoding analyses are key to not bias the total phycobiont diversity detected. This study highlights that inclusion of HTS analysis is crucial to understand lichen symbiotic microalgal diversity. |
| first_indexed | 2024-03-10T11:11:16Z |
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| id | doaj.art-65e022f6c0094c1aa29e47ed88808b68 |
| institution | Directory Open Access Journal |
| issn | 1424-2818 |
| language | English |
| last_indexed | 2024-03-10T11:11:16Z |
| publishDate | 2021-05-01 |
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| spelling | doaj.art-65e022f6c0094c1aa29e47ed88808b682023-11-21T20:45:15ZengMDPI AGDiversity1424-28182021-05-0113622010.3390/d13060220Unravelling the Symbiotic Microalgal Diversity in <i>Buellia zoharyi</i> (Lichenized Ascomycota) from the Iberian Peninsula and Balearic Islands Using DNA MetabarcodingPatricia Moya0Salvador Chiva1Arantzazu Molins2Isaac Garrido-Benavent3Eva Barreno4Botánica, ICBIBE, Fac. CC. Biológicas, Universitat de València, C/Dr. Moliner, 50, 46100 Valencia, SpainBotánica, ICBIBE, Fac. CC. Biológicas, Universitat de València, C/Dr. Moliner, 50, 46100 Valencia, SpainBotánica, ICBIBE, Fac. CC. Biológicas, Universitat de València, C/Dr. Moliner, 50, 46100 Valencia, SpainBotánica, ICBIBE, Fac. CC. Biológicas, Universitat de València, C/Dr. Moliner, 50, 46100 Valencia, SpainBotánica, ICBIBE, Fac. CC. Biológicas, Universitat de València, C/Dr. Moliner, 50, 46100 Valencia, Spain<i>Buellia zoharyi</i> is a crustose placodioid lichen, usually occurring on biocrusts of semiarid ecosystems in circum-Mediterranean/Macaronesian areas. In previous work, we found that this lichenized fungus was flexible in its phycobiont choice in the Canary Islands. Here we test whether geography and habitat influence phycobiont diversity in populations of this lichen from the Iberian Peninsula and Balearic Islands using Sanger and high throughput sequencing (HTS). Additionally, three thallus section categories (central, middle and periphery) were analyzed to explore diversity of microalgal communities in each part. We found that <i>B. zoharyi</i> populations hosted at least three different <i>Trebouxia</i> spp., and this lichen can associate with distinct phycobiont strains in different habitats and geographic regions. This study also revealed that the <i>Trebouxia</i> composition of this lichen showed significant differences when comparing the Iberian Peninsula with the Balearics thalli. No support for differences in microalgal communities was found among thallus sections; however, several thalli showed different predominant <i>Trebouxia</i> spp. at each section. This result corroborate that thallus parts selected for DNA extraction in metabarcoding analyses are key to not bias the total phycobiont diversity detected. This study highlights that inclusion of HTS analysis is crucial to understand lichen symbiotic microalgal diversity.https://www.mdpi.com/1424-2818/13/6/220coexistencehigh throughput sequencingIlluminametabarcodingsymbiosis<i>Trebouxia</i> |
| spellingShingle | Patricia Moya Salvador Chiva Arantzazu Molins Isaac Garrido-Benavent Eva Barreno Unravelling the Symbiotic Microalgal Diversity in <i>Buellia zoharyi</i> (Lichenized Ascomycota) from the Iberian Peninsula and Balearic Islands Using DNA Metabarcoding Diversity coexistence high throughput sequencing Illumina metabarcoding symbiosis <i>Trebouxia</i> |
| title | Unravelling the Symbiotic Microalgal Diversity in <i>Buellia zoharyi</i> (Lichenized Ascomycota) from the Iberian Peninsula and Balearic Islands Using DNA Metabarcoding |
| title_full | Unravelling the Symbiotic Microalgal Diversity in <i>Buellia zoharyi</i> (Lichenized Ascomycota) from the Iberian Peninsula and Balearic Islands Using DNA Metabarcoding |
| title_fullStr | Unravelling the Symbiotic Microalgal Diversity in <i>Buellia zoharyi</i> (Lichenized Ascomycota) from the Iberian Peninsula and Balearic Islands Using DNA Metabarcoding |
| title_full_unstemmed | Unravelling the Symbiotic Microalgal Diversity in <i>Buellia zoharyi</i> (Lichenized Ascomycota) from the Iberian Peninsula and Balearic Islands Using DNA Metabarcoding |
| title_short | Unravelling the Symbiotic Microalgal Diversity in <i>Buellia zoharyi</i> (Lichenized Ascomycota) from the Iberian Peninsula and Balearic Islands Using DNA Metabarcoding |
| title_sort | unravelling the symbiotic microalgal diversity in i buellia zoharyi i lichenized ascomycota from the iberian peninsula and balearic islands using dna metabarcoding |
| topic | coexistence high throughput sequencing Illumina metabarcoding symbiosis <i>Trebouxia</i> |
| url | https://www.mdpi.com/1424-2818/13/6/220 |
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