Arabidopsis peroxisome proteomics
The analytical depth of investigation of the peroxisomal proteome of the model plant Arabidopsis thaliana has not yet reached that of other major cellular organelles such as chloroplasts or mitochondria. This is primarily due to the difficulties associated with isolating and obtaining purified sampl...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2013-04-01
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| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2013.00101/full |
| _version_ | 1819228719843115008 |
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| author | John D. Bussell Christof eBehrens Wiebke eEcke Holger eEubel |
| author_facet | John D. Bussell Christof eBehrens Wiebke eEcke Holger eEubel |
| author_sort | John D. Bussell |
| collection | DOAJ |
| description | The analytical depth of investigation of the peroxisomal proteome of the model plant Arabidopsis thaliana has not yet reached that of other major cellular organelles such as chloroplasts or mitochondria. This is primarily due to the difficulties associated with isolating and obtaining purified samples of peroxisomes from Arabidopsis. So far only a handful of research groups have been successful in obtaining such fractions. To make things worse, enriched peroxisome fractions frequently suffer from significant organellar contamination, lowering confidence in localization assignment of the identified proteins. As with other cellular compartments, identification of peroxisomal proteins forms the basis for investigations of the dynamics of the peroxisomal proteome. It is therefore not surprising that, in terms of functional analyses by proteomic means, there remains a considerable gap between peroxisomes and chloroplasts or mitochondria. Alternative strategies are needed to overcome the obstacle of hard-to-obtain organellar fractions. This will help to close the knowledge gap between peroxisomes and other organelles and provide a full picture of the physiological pathways shared between organelles. In this review we briefly summarize the status quo and discuss some of the methodological alternatives to classic organelle proteomic approaches. |
| first_indexed | 2024-12-23T11:01:45Z |
| format | Article |
| id | doaj.art-65e0ecdded664507ad65cef34cb7178a |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-23T11:01:45Z |
| publishDate | 2013-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-65e0ecdded664507ad65cef34cb7178a2022-12-21T17:49:36ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2013-04-01410.3389/fpls.2013.0010141930Arabidopsis peroxisome proteomicsJohn D. Bussell0Christof eBehrens1Wiebke eEcke2Holger eEubel3The University of Western AustraliaLeibniz Universität HannoverLeibniz Universität HannoverLeibniz Universität HannoverThe analytical depth of investigation of the peroxisomal proteome of the model plant Arabidopsis thaliana has not yet reached that of other major cellular organelles such as chloroplasts or mitochondria. This is primarily due to the difficulties associated with isolating and obtaining purified samples of peroxisomes from Arabidopsis. So far only a handful of research groups have been successful in obtaining such fractions. To make things worse, enriched peroxisome fractions frequently suffer from significant organellar contamination, lowering confidence in localization assignment of the identified proteins. As with other cellular compartments, identification of peroxisomal proteins forms the basis for investigations of the dynamics of the peroxisomal proteome. It is therefore not surprising that, in terms of functional analyses by proteomic means, there remains a considerable gap between peroxisomes and chloroplasts or mitochondria. Alternative strategies are needed to overcome the obstacle of hard-to-obtain organellar fractions. This will help to close the knowledge gap between peroxisomes and other organelles and provide a full picture of the physiological pathways shared between organelles. In this review we briefly summarize the status quo and discuss some of the methodological alternatives to classic organelle proteomic approaches.http://journal.frontiersin.org/Journal/10.3389/fpls.2013.00101/fullperoxisomeSubcellular localizationfree flow electrophoresisfunctional proteomicsprotein:protein interactiontargeted quantitation of proteins |
| spellingShingle | John D. Bussell Christof eBehrens Wiebke eEcke Holger eEubel Arabidopsis peroxisome proteomics Frontiers in Plant Science peroxisome Subcellular localization free flow electrophoresis functional proteomics protein:protein interaction targeted quantitation of proteins |
| title | Arabidopsis peroxisome proteomics |
| title_full | Arabidopsis peroxisome proteomics |
| title_fullStr | Arabidopsis peroxisome proteomics |
| title_full_unstemmed | Arabidopsis peroxisome proteomics |
| title_short | Arabidopsis peroxisome proteomics |
| title_sort | arabidopsis peroxisome proteomics |
| topic | peroxisome Subcellular localization free flow electrophoresis functional proteomics protein:protein interaction targeted quantitation of proteins |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2013.00101/full |
| work_keys_str_mv | AT johndbussell arabidopsisperoxisomeproteomics AT christofebehrens arabidopsisperoxisomeproteomics AT wiebkeeecke arabidopsisperoxisomeproteomics AT holgereeubel arabidopsisperoxisomeproteomics |