Expression of major ectonucleotidases after cortical stab brain injury in rats: A real-time PCR study
Ectonucleotidases are cell surface-located enzymes responsible for the extracellular degradation of nucleotides. They are comprised of several protein families: ectonucleoside triphosphate diphosphohydrolases (E-NTPDase), ectonucleotide pyrophosphatase/phosphodiesterases (E-NPPases) and...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
University of Belgrade, University of Novi Sad
2014-01-01
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| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2014/0354-46641401149P.pdf |
| Summary: | Ectonucleotidases are cell surface-located enzymes responsible for the
extracellular degradation of nucleotides. They are comprised of several
protein families: ectonucleoside triphosphate diphosphohydrolases
(E-NTPDase), ectonucleotide pyrophosphatase/phosphodiesterases (E-NPPases)
and ecto-5’-nucleotidase. Previously we showed that cortical stab injury
alters ectonucleotidase activities in the rat brain, but that the specific
enzymes responsible for these changes were not identified. In this study we
investigated the gene expression of the specific ectonucleotidase enzymes,
NTPDase1- 3, NPP1-3 and ecto-5’-nucleotidase, two and seven days after
cortical stab injury in rats, using real-time PCR. Two days after the injury
we observed only one significant change: the downregulation in NTPDase2 mRNA
expression. Our results indicate that traumatic brain injury induces
significant upregulation of NTPDase1, NTPDase2 and ecto-5’-nucleotidase
transcripts, and the downregulation of NPP1, seven days after the injury.
Thus, traumatic brain injury has diverse impacts on ectonucleotidases gene
expression, which may be reflected in the enzyme activities and extracellular
nucleotide concentrations in the perilesional tissue. [Projekat Ministarstva
nauke Republike Srbije, br. III41014] |
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| ISSN: | 0354-4664 1821-4339 |