Relationship between chromatin configuration and in vitro maturation ability in guinea pig oocytes
Abstract Background Germinal vesicle (GV) chromatin configurations of oocytes are proposed to be related to oocyte competence and may reflect the quality of oocyte. Currently, a limited number of published studies investigated the GV chromatin configurations of guinea pig oocytes. Objective In this...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-11-01
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| Series: | Veterinary Medicine and Science |
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| Online Access: | https://doi.org/10.1002/vms3.596 |
| _version_ | 1818995646358618112 |
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| author | Min‐Hua Yao Wan‐Jing Cheng Li‐Wei Liu Hui Zheng Wan‐Ying Gu Fang Miao Jing‐Fang Zhang Li Wang Yan‐Ping Su Ya‐Ling Liu Hong‐Shu Sui |
| author_facet | Min‐Hua Yao Wan‐Jing Cheng Li‐Wei Liu Hui Zheng Wan‐Ying Gu Fang Miao Jing‐Fang Zhang Li Wang Yan‐Ping Su Ya‐Ling Liu Hong‐Shu Sui |
| author_sort | Min‐Hua Yao |
| collection | DOAJ |
| description | Abstract Background Germinal vesicle (GV) chromatin configurations of oocytes are proposed to be related to oocyte competence and may reflect the quality of oocyte. Currently, a limited number of published studies investigated the GV chromatin configurations of guinea pig oocytes. Objective In this study on the in vitro maturation (IVM) of guinea pig oocytes, we examined the changes in their GV chromatin configurations during meiotic progression. Methods Based on the degree of chromatin compaction, the GV chromatin configurations of guinea pig oocytes could be divided into three categories depending on whether the nucleolus‐like body (NLB) was surrounded or partly surrounded by compacted chromatin, namely the uncondensed (NSN), the intermediate type (SN‐1) and the compacted type (SN‐2). Results The percentage of cells displaying the SN‐2 configuration increased with the growth of guinea pig oocytes, suggesting that this configuration presents the potential for maturation in oocytes. Oocytes derived from larger follicle exhibited increased meiotic potential. Serum starvation affected the GV chromatin configurations of guinea pig oocytes. Conclusions Collectively, these results suggest that the SN‐2 type might be a more mature form of configuration in guinea pig oocyte, whose proportion was associated with the follicle size and susceptible to the environment (e.g. serum concentration). |
| first_indexed | 2024-12-20T21:17:09Z |
| format | Article |
| id | doaj.art-65e19e9147724b3cb9c8fb9c3f3f01eb |
| institution | Directory Open Access Journal |
| issn | 2053-1095 |
| language | English |
| last_indexed | 2024-12-20T21:17:09Z |
| publishDate | 2021-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine and Science |
| spelling | doaj.art-65e19e9147724b3cb9c8fb9c3f3f01eb2022-12-21T19:26:22ZengWileyVeterinary Medicine and Science2053-10952021-11-01762410241710.1002/vms3.596Relationship between chromatin configuration and in vitro maturation ability in guinea pig oocytesMin‐Hua Yao0Wan‐Jing Cheng1Li‐Wei Liu2Hui Zheng3Wan‐Ying Gu4Fang Miao5Jing‐Fang Zhang6Li Wang7Yan‐Ping Su8Ya‐Ling Liu9Hong‐Shu Sui10Department of Histology and Embryology Shandong First Medical University & Shandong Academy of Medical Sciences Tai'an Shandong Province ChinaDepartment of Pathology Hainan General Hospital Haikou Hainan Province ChinaDepartment of Histology and Embryology Shandong First Medical University & Shandong Academy of Medical Sciences Tai'an Shandong Province ChinaDepartment of Histology and Embryology Shandong First Medical University & Shandong Academy of Medical Sciences Tai'an Shandong Province ChinaDepartment of Histology and Embryology Shandong First Medical University & Shandong Academy of Medical Sciences Tai'an Shandong Province ChinaDepartment of Pathology Shandong First Medical University & Shandong Academy of Medical Sciences Tai'an Shandong Province ChinaDepartment of Pathology Shandong First Medical University & Shandong Academy of Medical Sciences Tai'an Shandong Province ChinaDepartment of Pathology Shandong First Medical University & Shandong Academy of Medical Sciences Tai'an Shandong Province ChinaDepartment of Histology and Embryology Shandong First Medical University & Shandong Academy of Medical Sciences Tai'an Shandong Province ChinaDepartment of Pathology Shandong First Medical University & Shandong Academy of Medical Sciences Tai'an Shandong Province ChinaDepartment of Histology and Embryology Shandong First Medical University & Shandong Academy of Medical Sciences Tai'an Shandong Province ChinaAbstract Background Germinal vesicle (GV) chromatin configurations of oocytes are proposed to be related to oocyte competence and may reflect the quality of oocyte. Currently, a limited number of published studies investigated the GV chromatin configurations of guinea pig oocytes. Objective In this study on the in vitro maturation (IVM) of guinea pig oocytes, we examined the changes in their GV chromatin configurations during meiotic progression. Methods Based on the degree of chromatin compaction, the GV chromatin configurations of guinea pig oocytes could be divided into three categories depending on whether the nucleolus‐like body (NLB) was surrounded or partly surrounded by compacted chromatin, namely the uncondensed (NSN), the intermediate type (SN‐1) and the compacted type (SN‐2). Results The percentage of cells displaying the SN‐2 configuration increased with the growth of guinea pig oocytes, suggesting that this configuration presents the potential for maturation in oocytes. Oocytes derived from larger follicle exhibited increased meiotic potential. Serum starvation affected the GV chromatin configurations of guinea pig oocytes. Conclusions Collectively, these results suggest that the SN‐2 type might be a more mature form of configuration in guinea pig oocyte, whose proportion was associated with the follicle size and susceptible to the environment (e.g. serum concentration).https://doi.org/10.1002/vms3.596chromatin configurationcompetence of maturationgerminal vesicleguinea pig oocyte |
| spellingShingle | Min‐Hua Yao Wan‐Jing Cheng Li‐Wei Liu Hui Zheng Wan‐Ying Gu Fang Miao Jing‐Fang Zhang Li Wang Yan‐Ping Su Ya‐Ling Liu Hong‐Shu Sui Relationship between chromatin configuration and in vitro maturation ability in guinea pig oocytes Veterinary Medicine and Science chromatin configuration competence of maturation germinal vesicle guinea pig oocyte |
| title | Relationship between chromatin configuration and in vitro maturation ability in guinea pig oocytes |
| title_full | Relationship between chromatin configuration and in vitro maturation ability in guinea pig oocytes |
| title_fullStr | Relationship between chromatin configuration and in vitro maturation ability in guinea pig oocytes |
| title_full_unstemmed | Relationship between chromatin configuration and in vitro maturation ability in guinea pig oocytes |
| title_short | Relationship between chromatin configuration and in vitro maturation ability in guinea pig oocytes |
| title_sort | relationship between chromatin configuration and in vitro maturation ability in guinea pig oocytes |
| topic | chromatin configuration competence of maturation germinal vesicle guinea pig oocyte |
| url | https://doi.org/10.1002/vms3.596 |
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