Transcriptome profiling provides insights into leaf color changes in two Acer palmatum genotypes
Abstract Background Ornamental trees with seasonally-dependent leaf color, such as Acer palmatum, have gained worldwide popularity. Leaf color is a main determinant of the ornamental and economic value of A. palmatum. However, the molecular mechanisms responsible for leaf color changes remain unclea...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-12-01
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| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12870-022-03979-x |
| _version_ | 1798005004903120896 |
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| author | Lu Zhu Jing Wen Qiuyue Ma Kunyuan Yan Yiming Du Zhu Chen Xiaoyu Lu Jie Ren Yuelan Wang Shushun Li Qianzhong Li |
| author_facet | Lu Zhu Jing Wen Qiuyue Ma Kunyuan Yan Yiming Du Zhu Chen Xiaoyu Lu Jie Ren Yuelan Wang Shushun Li Qianzhong Li |
| author_sort | Lu Zhu |
| collection | DOAJ |
| description | Abstract Background Ornamental trees with seasonally-dependent leaf color, such as Acer palmatum, have gained worldwide popularity. Leaf color is a main determinant of the ornamental and economic value of A. palmatum. However, the molecular mechanisms responsible for leaf color changes remain unclear. Results We chose A. palmatum cultivars with yellow (‘Jinling Huangfeng’) and red (‘Jinling Danfeng’) leaves as the ideal material for studying the complex metabolic networks responsible for variations in leaf coloration. The 24 libraries obtained from four different time points in the growth of ‘Jinling Huangfeng’ and ‘Jinling Danfeng’ was subjected to Illumina high-throughput sequencing. We observed that the difference in cyanidin and delphinidin content is the primary reason behind the varying coloration of the leaves. Transcriptomic analyses revealed 225,684 unigenes, and the Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of differentially expressed genes (DEGs) confirmed that they were involved in ‘anthocyanin biosynthesis.’ Eighteen structural genes involved in anthocyanin biosynthesis were thought to be related to anthocyanin accumulation, whereas 46 MYBs, 33 basic helix-loop-helixs (bHLHs), and 29 WD40s were presumed to be involved in regulating anthocyanin biosynthesis. Based on weighted gene co-expression network analysis (WGCNA), three candidate genes (ApRHOMBOID, ApMAPK, and ApUNE10) were screened in the significant association module with a correlation coefficient (r 2 ) of 0.86. Conclusion In this study, the leaf color changes of two A. palmatum genotypes were analyzed. These findings provide novel insights into variations in leaf coloration and suggest pathways for targeted genetic improvements in A. palmatum. |
| first_indexed | 2024-04-11T12:32:18Z |
| format | Article |
| id | doaj.art-65e3a9b0ebc940fab1a76a5e406d6f97 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-04-11T12:32:18Z |
| publishDate | 2022-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-65e3a9b0ebc940fab1a76a5e406d6f972022-12-22T04:23:43ZengBMCBMC Plant Biology1471-22292022-12-0122111410.1186/s12870-022-03979-xTranscriptome profiling provides insights into leaf color changes in two Acer palmatum genotypesLu Zhu0Jing Wen1Qiuyue Ma2Kunyuan Yan3Yiming Du4Zhu Chen5Xiaoyu Lu6Jie Ren7Yuelan Wang8Shushun Li9Qianzhong Li10Institute of Leisure Agriculture, Jiangsu Academy of Agricultural SciencesInstitute of Leisure Agriculture, Jiangsu Academy of Agricultural SciencesInstitute of Leisure Agriculture, Jiangsu Academy of Agricultural SciencesInstitute of Leisure Agriculture, Jiangsu Academy of Agricultural SciencesInstitute of Leisure Agriculture, Jiangsu Academy of Agricultural SciencesInstitute of Agricultural Engineering, Anhui Academy of Agricultural SciencesInstitute of Agricultural Engineering, Anhui Academy of Agricultural SciencesInstitute of Agricultural Engineering, Anhui Academy of Agricultural SciencesChenshi Maples NurseryInstitute of Leisure Agriculture, Jiangsu Academy of Agricultural SciencesInstitute of Leisure Agriculture, Jiangsu Academy of Agricultural SciencesAbstract Background Ornamental trees with seasonally-dependent leaf color, such as Acer palmatum, have gained worldwide popularity. Leaf color is a main determinant of the ornamental and economic value of A. palmatum. However, the molecular mechanisms responsible for leaf color changes remain unclear. Results We chose A. palmatum cultivars with yellow (‘Jinling Huangfeng’) and red (‘Jinling Danfeng’) leaves as the ideal material for studying the complex metabolic networks responsible for variations in leaf coloration. The 24 libraries obtained from four different time points in the growth of ‘Jinling Huangfeng’ and ‘Jinling Danfeng’ was subjected to Illumina high-throughput sequencing. We observed that the difference in cyanidin and delphinidin content is the primary reason behind the varying coloration of the leaves. Transcriptomic analyses revealed 225,684 unigenes, and the Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of differentially expressed genes (DEGs) confirmed that they were involved in ‘anthocyanin biosynthesis.’ Eighteen structural genes involved in anthocyanin biosynthesis were thought to be related to anthocyanin accumulation, whereas 46 MYBs, 33 basic helix-loop-helixs (bHLHs), and 29 WD40s were presumed to be involved in regulating anthocyanin biosynthesis. Based on weighted gene co-expression network analysis (WGCNA), three candidate genes (ApRHOMBOID, ApMAPK, and ApUNE10) were screened in the significant association module with a correlation coefficient (r 2 ) of 0.86. Conclusion In this study, the leaf color changes of two A. palmatum genotypes were analyzed. These findings provide novel insights into variations in leaf coloration and suggest pathways for targeted genetic improvements in A. palmatum.https://doi.org/10.1186/s12870-022-03979-xAcer palmatumTranscriptomeLeaf colorationAnthocyaninTranscription factors |
| spellingShingle | Lu Zhu Jing Wen Qiuyue Ma Kunyuan Yan Yiming Du Zhu Chen Xiaoyu Lu Jie Ren Yuelan Wang Shushun Li Qianzhong Li Transcriptome profiling provides insights into leaf color changes in two Acer palmatum genotypes BMC Plant Biology Acer palmatum Transcriptome Leaf coloration Anthocyanin Transcription factors |
| title | Transcriptome profiling provides insights into leaf color changes in two Acer palmatum genotypes |
| title_full | Transcriptome profiling provides insights into leaf color changes in two Acer palmatum genotypes |
| title_fullStr | Transcriptome profiling provides insights into leaf color changes in two Acer palmatum genotypes |
| title_full_unstemmed | Transcriptome profiling provides insights into leaf color changes in two Acer palmatum genotypes |
| title_short | Transcriptome profiling provides insights into leaf color changes in two Acer palmatum genotypes |
| title_sort | transcriptome profiling provides insights into leaf color changes in two acer palmatum genotypes |
| topic | Acer palmatum Transcriptome Leaf coloration Anthocyanin Transcription factors |
| url | https://doi.org/10.1186/s12870-022-03979-x |
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