Anti-Ischemic Effect of Leptin in the Isolated Rat Heart Subjected to Global Ischemia-Reperfusion: Role of Cardiac-Specific miRNAs
Background: Leptin is an obesity-associated adipokine that has been implicated in cardiac protection against ischemia-reperfusion injury (IRI). In this study, concentration-dependent effects of leptin on myocardial IRI were investigated in the isolated rat heart. In addition, we analyzed myocardial...
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MDPI AG
2023-01-01
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| Series: | Cardiogenetics |
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| Online Access: | https://www.mdpi.com/2035-8148/13/1/1 |
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| author | Ekaterina A. Polyakova Evgeny N. Mikhaylov Sarkis M. Minasian Mikhail M. Galagudza Evgeny V. Shlyakhto |
| author_facet | Ekaterina A. Polyakova Evgeny N. Mikhaylov Sarkis M. Minasian Mikhail M. Galagudza Evgeny V. Shlyakhto |
| author_sort | Ekaterina A. Polyakova |
| collection | DOAJ |
| description | Background: Leptin is an obesity-associated adipokine that has been implicated in cardiac protection against ischemia-reperfusion injury (IRI). In this study, concentration-dependent effects of leptin on myocardial IRI were investigated in the isolated rat heart. In addition, we analyzed myocardial miRNAs expression in order to investigate their potential involvement in leptin-mediated cardioprotection. Methods: The effect of leptin on IRI was examined in Langendorff-perfused rat hearts preconditioned with two leptin concentrations (1.0 nM and 3.1 nM) for 60 min. The hearts were subjected to 30 min global ischemia and 120 min reperfusion with buffer containing leptin in the respective concentration. Heart function and arrhythmia incidence were analyzed. Infarct size was assessed histochemically. Expression of miRNA-144, -208a, -378, and -499 was analyzed in the ventricular myocardium using RT-PCR. Results: The addition of 1.0 nM leptin to the buffer exerted an infarct-limiting effect, preserved post-ischemic ventricular function, and prevented reperfusion arrhythmia compared to 3.1 nM leptin. Myocardial expression of miRNA-208a was decreased after heart exposure to 1.0 nM leptin and significantly elevated in the hearts perfused with leptin at 3.1 nM. Conclusion: Acute administration of leptin at low dose (1.0 nM) results in cardiac protection against IRI. This effect is associated with reduced myocardial expression of miRNA-208a. |
| first_indexed | 2024-04-09T21:14:05Z |
| format | Article |
| id | doaj.art-65e7e1f3dd0b4dc6b0014f8961988f52 |
| institution | Directory Open Access Journal |
| issn | 2035-8253 2035-8148 |
| language | English |
| last_indexed | 2024-04-09T21:14:05Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cardiogenetics |
| spelling | doaj.art-65e7e1f3dd0b4dc6b0014f8961988f522023-03-28T13:17:49ZengMDPI AGCardiogenetics2035-82532035-81482023-01-0113111310.3390/cardiogenetics13010001Anti-Ischemic Effect of Leptin in the Isolated Rat Heart Subjected to Global Ischemia-Reperfusion: Role of Cardiac-Specific miRNAsEkaterina A. Polyakova0Evgeny N. Mikhaylov1Sarkis M. Minasian2Mikhail M. Galagudza3Evgeny V. Shlyakhto4Almazov National Medical Research Centre, Institute of Experimental Medicine, 197341 Saint-Petersburg, RussiaAlmazov National Medical Research Centre, Institute of Experimental Medicine, 197341 Saint-Petersburg, RussiaAlmazov National Medical Research Centre, Institute of Experimental Medicine, 197341 Saint-Petersburg, RussiaAlmazov National Medical Research Centre, Institute of Experimental Medicine, 197341 Saint-Petersburg, RussiaAlmazov National Medical Research Centre, Institute of Experimental Medicine, 197341 Saint-Petersburg, RussiaBackground: Leptin is an obesity-associated adipokine that has been implicated in cardiac protection against ischemia-reperfusion injury (IRI). In this study, concentration-dependent effects of leptin on myocardial IRI were investigated in the isolated rat heart. In addition, we analyzed myocardial miRNAs expression in order to investigate their potential involvement in leptin-mediated cardioprotection. Methods: The effect of leptin on IRI was examined in Langendorff-perfused rat hearts preconditioned with two leptin concentrations (1.0 nM and 3.1 nM) for 60 min. The hearts were subjected to 30 min global ischemia and 120 min reperfusion with buffer containing leptin in the respective concentration. Heart function and arrhythmia incidence were analyzed. Infarct size was assessed histochemically. Expression of miRNA-144, -208a, -378, and -499 was analyzed in the ventricular myocardium using RT-PCR. Results: The addition of 1.0 nM leptin to the buffer exerted an infarct-limiting effect, preserved post-ischemic ventricular function, and prevented reperfusion arrhythmia compared to 3.1 nM leptin. Myocardial expression of miRNA-208a was decreased after heart exposure to 1.0 nM leptin and significantly elevated in the hearts perfused with leptin at 3.1 nM. Conclusion: Acute administration of leptin at low dose (1.0 nM) results in cardiac protection against IRI. This effect is associated with reduced myocardial expression of miRNA-208a.https://www.mdpi.com/2035-8148/13/1/1leptinheartischemiareperfusioninfarct sizerat |
| spellingShingle | Ekaterina A. Polyakova Evgeny N. Mikhaylov Sarkis M. Minasian Mikhail M. Galagudza Evgeny V. Shlyakhto Anti-Ischemic Effect of Leptin in the Isolated Rat Heart Subjected to Global Ischemia-Reperfusion: Role of Cardiac-Specific miRNAs Cardiogenetics leptin heart ischemia reperfusion infarct size rat |
| title | Anti-Ischemic Effect of Leptin in the Isolated Rat Heart Subjected to Global Ischemia-Reperfusion: Role of Cardiac-Specific miRNAs |
| title_full | Anti-Ischemic Effect of Leptin in the Isolated Rat Heart Subjected to Global Ischemia-Reperfusion: Role of Cardiac-Specific miRNAs |
| title_fullStr | Anti-Ischemic Effect of Leptin in the Isolated Rat Heart Subjected to Global Ischemia-Reperfusion: Role of Cardiac-Specific miRNAs |
| title_full_unstemmed | Anti-Ischemic Effect of Leptin in the Isolated Rat Heart Subjected to Global Ischemia-Reperfusion: Role of Cardiac-Specific miRNAs |
| title_short | Anti-Ischemic Effect of Leptin in the Isolated Rat Heart Subjected to Global Ischemia-Reperfusion: Role of Cardiac-Specific miRNAs |
| title_sort | anti ischemic effect of leptin in the isolated rat heart subjected to global ischemia reperfusion role of cardiac specific mirnas |
| topic | leptin heart ischemia reperfusion infarct size rat |
| url | https://www.mdpi.com/2035-8148/13/1/1 |
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