Plasma Exosome Profile in ST-Elevation Myocardial Infarction Patients with and without Out-of-Hospital Cardiac Arrest
The identification of new biomarkers allowing an early and more accurate characterization of patients with ST-segment elevation myocardial infarction (STEMI) is still needed, and exosomes represent an attractive diagnostic tool in this context. However, the characterization of their protein cargo in...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-07-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/15/8065 |
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| author | Marta Zarà Jeness Campodonico Nicola Cosentino Maria Luisa Biondi Patrizia Amadio Gloria Milanesi Emilio Assanelli Silvia Cerri Marco Biggiogera Leonardo Sandrini Calogero Claudio Tedesco Fabrizio Veglia Daniela Trabattoni Fabio Blandini Elena Tremoli Giancarlo Marenzi Silvia S. Barbieri |
| author_facet | Marta Zarà Jeness Campodonico Nicola Cosentino Maria Luisa Biondi Patrizia Amadio Gloria Milanesi Emilio Assanelli Silvia Cerri Marco Biggiogera Leonardo Sandrini Calogero Claudio Tedesco Fabrizio Veglia Daniela Trabattoni Fabio Blandini Elena Tremoli Giancarlo Marenzi Silvia S. Barbieri |
| author_sort | Marta Zarà |
| collection | DOAJ |
| description | The identification of new biomarkers allowing an early and more accurate characterization of patients with ST-segment elevation myocardial infarction (STEMI) is still needed, and exosomes represent an attractive diagnostic tool in this context. However, the characterization of their protein cargo in relation to cardiovascular clinical manifestation is still lacking. To this end, 35 STEMI patients (17 experiencing resuscitated out-of-hospital cardiac arrest (OHCA-STEMI) and 18 uncomplicated) and 32 patients with chronic coronary syndrome (CCS) were enrolled. Plasma exosomes were characterized by the nanoparticle tracking analysis and Western blotting. Exosomes from STEMI patients displayed a higher concentration and size and a greater expression of platelet (GPIIb) and vascular endothelial (VE-cadherin) markers, but a similar amount of cardiac troponin compared to CCS. In addition, a difference in exosome expression of acute-phase proteins (ceruloplasmin, transthyretin and fibronectin) between STEMI and CCS patients was found. GPIIb and brain-associated marker PLP1 accurately discriminated between OHCA and uncomplicated STEMI. In conclusion, the exosome profile of STEMI patients has peculiar features that differentiate it from that of CCS patients, reflecting the pathophysiological mechanisms involved in STEMI. Additionally, the exosome expression of brain- and platelet-specific markers might allow the identification of patients experiencing ischemic brain injury in STEMI. |
| first_indexed | 2024-03-10T09:14:56Z |
| format | Article |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T09:14:56Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-65e7e993ae2c42508cf6da8f36207db02023-11-22T05:42:39ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-07-012215806510.3390/ijms22158065Plasma Exosome Profile in ST-Elevation Myocardial Infarction Patients with and without Out-of-Hospital Cardiac ArrestMarta Zarà0Jeness Campodonico1Nicola Cosentino2Maria Luisa Biondi3Patrizia Amadio4Gloria Milanesi5Emilio Assanelli6Silvia Cerri7Marco Biggiogera8Leonardo Sandrini9Calogero Claudio Tedesco10Fabrizio Veglia11Daniela Trabattoni12Fabio Blandini13Elena Tremoli14Giancarlo Marenzi15Silvia S. Barbieri16Centro Cardiologico Monzino IRCCS, 20138 Milan, ItalyCentro Cardiologico Monzino IRCCS, 20138 Milan, ItalyCentro Cardiologico Monzino IRCCS, 20138 Milan, ItalyCentro Cardiologico Monzino IRCCS, 20138 Milan, ItalyCentro Cardiologico Monzino IRCCS, 20138 Milan, ItalyDepartment of Biology and Biotechnology, University of Pavia, “L. Spallanzani”, 27100 Pavia, ItalyCentro Cardiologico Monzino IRCCS, 20138 Milan, ItalyMondino Foundation IRCCS, 27100 Pavia, ItalyDepartment of Biology and Biotechnology, University of Pavia, “L. Spallanzani”, 27100 Pavia, ItalyCentro Cardiologico Monzino IRCCS, 20138 Milan, ItalyCentro Cardiologico Monzino IRCCS, 20138 Milan, ItalyCentro Cardiologico Monzino IRCCS, 20138 Milan, ItalyCentro Cardiologico Monzino IRCCS, 20138 Milan, ItalyMondino Foundation IRCCS, 27100 Pavia, ItalyCentro Cardiologico Monzino IRCCS, 20138 Milan, ItalyCentro Cardiologico Monzino IRCCS, 20138 Milan, ItalyCentro Cardiologico Monzino IRCCS, 20138 Milan, ItalyThe identification of new biomarkers allowing an early and more accurate characterization of patients with ST-segment elevation myocardial infarction (STEMI) is still needed, and exosomes represent an attractive diagnostic tool in this context. However, the characterization of their protein cargo in relation to cardiovascular clinical manifestation is still lacking. To this end, 35 STEMI patients (17 experiencing resuscitated out-of-hospital cardiac arrest (OHCA-STEMI) and 18 uncomplicated) and 32 patients with chronic coronary syndrome (CCS) were enrolled. Plasma exosomes were characterized by the nanoparticle tracking analysis and Western blotting. Exosomes from STEMI patients displayed a higher concentration and size and a greater expression of platelet (GPIIb) and vascular endothelial (VE-cadherin) markers, but a similar amount of cardiac troponin compared to CCS. In addition, a difference in exosome expression of acute-phase proteins (ceruloplasmin, transthyretin and fibronectin) between STEMI and CCS patients was found. GPIIb and brain-associated marker PLP1 accurately discriminated between OHCA and uncomplicated STEMI. In conclusion, the exosome profile of STEMI patients has peculiar features that differentiate it from that of CCS patients, reflecting the pathophysiological mechanisms involved in STEMI. Additionally, the exosome expression of brain- and platelet-specific markers might allow the identification of patients experiencing ischemic brain injury in STEMI.https://www.mdpi.com/1422-0067/22/15/8065exosomesST-elevation myocardial infarctionresuscitated out-of-hospital cardiac arrestplateletsbrain-associated marker |
| spellingShingle | Marta Zarà Jeness Campodonico Nicola Cosentino Maria Luisa Biondi Patrizia Amadio Gloria Milanesi Emilio Assanelli Silvia Cerri Marco Biggiogera Leonardo Sandrini Calogero Claudio Tedesco Fabrizio Veglia Daniela Trabattoni Fabio Blandini Elena Tremoli Giancarlo Marenzi Silvia S. Barbieri Plasma Exosome Profile in ST-Elevation Myocardial Infarction Patients with and without Out-of-Hospital Cardiac Arrest International Journal of Molecular Sciences exosomes ST-elevation myocardial infarction resuscitated out-of-hospital cardiac arrest platelets brain-associated marker |
| title | Plasma Exosome Profile in ST-Elevation Myocardial Infarction Patients with and without Out-of-Hospital Cardiac Arrest |
| title_full | Plasma Exosome Profile in ST-Elevation Myocardial Infarction Patients with and without Out-of-Hospital Cardiac Arrest |
| title_fullStr | Plasma Exosome Profile in ST-Elevation Myocardial Infarction Patients with and without Out-of-Hospital Cardiac Arrest |
| title_full_unstemmed | Plasma Exosome Profile in ST-Elevation Myocardial Infarction Patients with and without Out-of-Hospital Cardiac Arrest |
| title_short | Plasma Exosome Profile in ST-Elevation Myocardial Infarction Patients with and without Out-of-Hospital Cardiac Arrest |
| title_sort | plasma exosome profile in st elevation myocardial infarction patients with and without out of hospital cardiac arrest |
| topic | exosomes ST-elevation myocardial infarction resuscitated out-of-hospital cardiac arrest platelets brain-associated marker |
| url | https://www.mdpi.com/1422-0067/22/15/8065 |
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