Differential expression of microRNA501-5p affects the aggressiveness of clear cell renal carcinoma
Renal cell carcinoma is a common neoplasia of the adult kidney that accounts for about 3% of adult malignancies. Clear cell renal carcinoma is the most frequent subtype of kidney cancer and 20–40% of patients develop metastases. The absence of appropriate biomarkers complicates diagnosis and prognos...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | FEBS Open Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211546314001053 |
| _version_ | 1818511687927463936 |
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| author | Alessandra Mangolini Anna Bonon Stefano Volinia Giovanni Lanza Roberto Gambari Paolo Pinton Gian Rosario Russo Laura del Senno Lucio Dell’Atti Gianluca Aguiari |
| author_facet | Alessandra Mangolini Anna Bonon Stefano Volinia Giovanni Lanza Roberto Gambari Paolo Pinton Gian Rosario Russo Laura del Senno Lucio Dell’Atti Gianluca Aguiari |
| author_sort | Alessandra Mangolini |
| collection | DOAJ |
| description | Renal cell carcinoma is a common neoplasia of the adult kidney that accounts for about 3% of adult malignancies. Clear cell renal carcinoma is the most frequent subtype of kidney cancer and 20–40% of patients develop metastases. The absence of appropriate biomarkers complicates diagnosis and prognosis of this disease. In this regard, small noncoding RNAs (microRNAs), which are mutated in several neoplastic diseases including kidney carcinoma, may be optimal candidates as biomarkers for diagnosis and prognosis of this kind of cancer. Here we show that patients with clear cell kidney carcinoma that express low levels of miR501-5p exhibited a good prognosis compared with patients with unchanged or high levels of this microRNA. Consistently, in kidney carcinoma cells the downregulation of miR501-5p induced an increased caspase-3 activity, p53 expression as well as decreased mTOR activation, leading to stimulation of the apoptotic pathway. Conversely, miR501-5p upregulation enhanced the activity of mTOR and promoted both cell proliferation and survival. These biological processes occurred through p53 inactivation by proteasome degradation in a mechanism involving MDM2-mediated p53 ubiquitination. Our results support a role for miR501-5p in balancing apoptosis and cell survival in clear cell renal carcinoma. In particular, the downregulation of microRNA501-5p promotes a good prognosis, while its upregulation contributes to a poor prognosis, in particular, if associated with p53 and MDM2 overexpression and mTOR activation. Thus, the expression of miR501-5p is a possible biomarker for the prognosis of clear cell renal carcinoma. |
| first_indexed | 2024-12-10T23:36:23Z |
| format | Article |
| id | doaj.art-65e83e85a0e0438897a20b7098d8ea3b |
| institution | Directory Open Access Journal |
| issn | 2211-5463 |
| language | English |
| last_indexed | 2024-12-10T23:36:23Z |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj.art-65e83e85a0e0438897a20b7098d8ea3b2022-12-22T01:29:10ZengWileyFEBS Open Bio2211-54632014-01-014C95296510.1016/j.fob.2014.10.016Differential expression of microRNA501-5p affects the aggressiveness of clear cell renal carcinomaAlessandra Mangolini0Anna Bonon1Stefano Volinia2Giovanni Lanza3Roberto Gambari4Paolo Pinton5Gian Rosario Russo6Laura del Senno7Lucio Dell’Atti8Gianluca Aguiari9Department of Morphology, Surgery and Experimental Medicine, Section of Pathology, Oncology and Experimental Biology, University of Ferrara, Via Fossato di Mortara 64/b, 44121 Ferrara, ItalyDepartment of Biomedical and Specialty Surgical Sciences, Section of Biochemistry, Molecular Biology and Medical Genetics, University of Ferrara, Via Fossato di Mortara 74, 44121 Ferrara, ItalyDepartment of Morphology, Surgery and Experimental Medicine, Section of Anatomy and Histology, University of Ferrara, Via Fossato di Mortara 64/b, 44121 Ferrara, ItalyDepartment of Morphology, Surgery and Experimental Medicine, Section of Pathological Anatomy and Biomolecular Diagnostic, Azienda Ospedaliero Universitaria, Via Aldo Moro 8, 44124 Ferrara, ItalyDepartment of Life Sciences and Biotechnologies, Section of Molecular Biology, University of Ferrara, Via Fossato di Mortara 74, 44121 Ferrara, ItalyDepartment of Morphology, Surgery and Experimental Medicine, Section of Pathology, Oncology and Experimental Biology, University of Ferrara, Via Fossato di Mortara 64/b, 44121 Ferrara, ItalyUnit of Urology, St. Anna Hospital, Via Aldo Moro 8, 44124 Ferrara, ItalyDepartment of Biomedical and Specialty Surgical Sciences, Section of Biochemistry, Molecular Biology and Medical Genetics, University of Ferrara, Via Fossato di Mortara 74, 44121 Ferrara, ItalyUnit of Urology, St. Anna Hospital, Via Aldo Moro 8, 44124 Ferrara, ItalyDepartment of Biomedical and Specialty Surgical Sciences, Section of Biochemistry, Molecular Biology and Medical Genetics, University of Ferrara, Via Fossato di Mortara 74, 44121 Ferrara, ItalyRenal cell carcinoma is a common neoplasia of the adult kidney that accounts for about 3% of adult malignancies. Clear cell renal carcinoma is the most frequent subtype of kidney cancer and 20–40% of patients develop metastases. The absence of appropriate biomarkers complicates diagnosis and prognosis of this disease. In this regard, small noncoding RNAs (microRNAs), which are mutated in several neoplastic diseases including kidney carcinoma, may be optimal candidates as biomarkers for diagnosis and prognosis of this kind of cancer. Here we show that patients with clear cell kidney carcinoma that express low levels of miR501-5p exhibited a good prognosis compared with patients with unchanged or high levels of this microRNA. Consistently, in kidney carcinoma cells the downregulation of miR501-5p induced an increased caspase-3 activity, p53 expression as well as decreased mTOR activation, leading to stimulation of the apoptotic pathway. Conversely, miR501-5p upregulation enhanced the activity of mTOR and promoted both cell proliferation and survival. These biological processes occurred through p53 inactivation by proteasome degradation in a mechanism involving MDM2-mediated p53 ubiquitination. Our results support a role for miR501-5p in balancing apoptosis and cell survival in clear cell renal carcinoma. In particular, the downregulation of microRNA501-5p promotes a good prognosis, while its upregulation contributes to a poor prognosis, in particular, if associated with p53 and MDM2 overexpression and mTOR activation. Thus, the expression of miR501-5p is a possible biomarker for the prognosis of clear cell renal carcinoma.http://www.sciencedirect.com/science/article/pii/S2211546314001053MicroRNA501-5pmTOR signalingp53ApoptosisCell survival |
| spellingShingle | Alessandra Mangolini Anna Bonon Stefano Volinia Giovanni Lanza Roberto Gambari Paolo Pinton Gian Rosario Russo Laura del Senno Lucio Dell’Atti Gianluca Aguiari Differential expression of microRNA501-5p affects the aggressiveness of clear cell renal carcinoma FEBS Open Bio MicroRNA501-5p mTOR signaling p53 Apoptosis Cell survival |
| title | Differential expression of microRNA501-5p affects the aggressiveness of clear cell renal carcinoma |
| title_full | Differential expression of microRNA501-5p affects the aggressiveness of clear cell renal carcinoma |
| title_fullStr | Differential expression of microRNA501-5p affects the aggressiveness of clear cell renal carcinoma |
| title_full_unstemmed | Differential expression of microRNA501-5p affects the aggressiveness of clear cell renal carcinoma |
| title_short | Differential expression of microRNA501-5p affects the aggressiveness of clear cell renal carcinoma |
| title_sort | differential expression of microrna501 5p affects the aggressiveness of clear cell renal carcinoma |
| topic | MicroRNA501-5p mTOR signaling p53 Apoptosis Cell survival |
| url | http://www.sciencedirect.com/science/article/pii/S2211546314001053 |
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