Novel Effective Yeast Strains and Their Performance in High Gravity and Very High Gravity Ethanol Fermentations from Sweet Sorghum Juice
Yeasts were isolated from four potential sources, sweet sorghum juice, sugar cane juice, grapes and rambutan. The 27 yeast isolates were tested for their ethanol tolerance (15% <i>v</i>/<i>v</i> of ethanol) and ethanol fermentation performance in a synthetic ethanol productio...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-01-01
|
| Series: | Energies |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1996-1073/14/3/557 |
| _version_ | 1797408503053156352 |
|---|---|
| author | Lakkana Laopaiboon Suntaree Suporn Preekamol Klanrit Niphaphat Phukoetphim Chalida Daengbussadee Pattana Laopaiboon |
| author_facet | Lakkana Laopaiboon Suntaree Suporn Preekamol Klanrit Niphaphat Phukoetphim Chalida Daengbussadee Pattana Laopaiboon |
| author_sort | Lakkana Laopaiboon |
| collection | DOAJ |
| description | Yeasts were isolated from four potential sources, sweet sorghum juice, sugar cane juice, grapes and rambutan. The 27 yeast isolates were tested for their ethanol tolerance (15% <i>v</i>/<i>v</i> of ethanol) and ethanol fermentation performance in a synthetic ethanol production medium (200 g/L of total sugar). Only five isolates, SCJ04KKU, SCJ07KKU, SCJ09KKU, SCJ14KKU and SSJ01KKU could tolerate 15% ethanol and produce ethanol at levels higher than 55 g/L. The ethanol production efficiency from sweet sorghum juice under high gravity (HG, 200 and 240 g/L of total sugar) and very high gravity (VHG, 280 g/L of total sugar) conditions of the five isolates was tested. <i>Saccharomyces cerevisiae</i> NP01 and <i>S. cerevisiae</i> ATCC4132 were used as reference strains. The results showed that the SSJ01KKU isolate gave the highest ethanol production efficiency under all conditions. Ethanol concentration (<i>P<sub>E</sub></i>), yield (<i>Y<sub>P/S</sub></i>) and productivity (<i>Q<sub>P</sub></i>) values were 98.89 g/L, 0.50 and 1.18 g/L·h, respectively, with sugar consumption (<i>SC</i>) of 98.96% under the HG condition at 200 g/L of total sugar. Under the HG condition at 240 g/L of total sugar, the <i>P<sub>E</sub></i>, <i>Y<sub>P/S</sub></i> and <i>Q<sub>P</sub></i> values were 118.12 g/L, 0.51 and 1.41 g/L·h, respectively, with the <i>SC</i> of 95.79%. These values were 82.29 g/L, 0.34 and 0.98 g/L·h, respectively, with the <i>SC</i> of 85.59% under the VHG condition. Addition of urea into the sweet sorghum juice under all conditions significantly shortened the fermentation time, resulting in increased <i>Q<sub>P</sub></i> values. Based on molecular taxonomic analysis of the five isolates using sequence analysis of the D1/D2 domain and the ITS1 and ITS2 regions, SSJ01KKU is <i>S. cerevisiae</i>, whereas SCJ04KKU, SCJ07KKU, SCJ09KKU and SCJ14KKU are <i>Pichia caribbica</i>. |
| first_indexed | 2024-03-09T04:00:25Z |
| format | Article |
| id | doaj.art-65eb56faf3c24052b728acb62ea6359a |
| institution | Directory Open Access Journal |
| issn | 1996-1073 |
| language | English |
| last_indexed | 2024-03-09T04:00:25Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Energies |
| spelling | doaj.art-65eb56faf3c24052b728acb62ea6359a2023-12-03T14:14:41ZengMDPI AGEnergies1996-10732021-01-0114355710.3390/en14030557Novel Effective Yeast Strains and Their Performance in High Gravity and Very High Gravity Ethanol Fermentations from Sweet Sorghum JuiceLakkana Laopaiboon0Suntaree Suporn1Preekamol Klanrit2Niphaphat Phukoetphim3Chalida Daengbussadee4Pattana Laopaiboon5Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, ThailandGraduate School, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, ThailandYeasts were isolated from four potential sources, sweet sorghum juice, sugar cane juice, grapes and rambutan. The 27 yeast isolates were tested for their ethanol tolerance (15% <i>v</i>/<i>v</i> of ethanol) and ethanol fermentation performance in a synthetic ethanol production medium (200 g/L of total sugar). Only five isolates, SCJ04KKU, SCJ07KKU, SCJ09KKU, SCJ14KKU and SSJ01KKU could tolerate 15% ethanol and produce ethanol at levels higher than 55 g/L. The ethanol production efficiency from sweet sorghum juice under high gravity (HG, 200 and 240 g/L of total sugar) and very high gravity (VHG, 280 g/L of total sugar) conditions of the five isolates was tested. <i>Saccharomyces cerevisiae</i> NP01 and <i>S. cerevisiae</i> ATCC4132 were used as reference strains. The results showed that the SSJ01KKU isolate gave the highest ethanol production efficiency under all conditions. Ethanol concentration (<i>P<sub>E</sub></i>), yield (<i>Y<sub>P/S</sub></i>) and productivity (<i>Q<sub>P</sub></i>) values were 98.89 g/L, 0.50 and 1.18 g/L·h, respectively, with sugar consumption (<i>SC</i>) of 98.96% under the HG condition at 200 g/L of total sugar. Under the HG condition at 240 g/L of total sugar, the <i>P<sub>E</sub></i>, <i>Y<sub>P/S</sub></i> and <i>Q<sub>P</sub></i> values were 118.12 g/L, 0.51 and 1.41 g/L·h, respectively, with the <i>SC</i> of 95.79%. These values were 82.29 g/L, 0.34 and 0.98 g/L·h, respectively, with the <i>SC</i> of 85.59% under the VHG condition. Addition of urea into the sweet sorghum juice under all conditions significantly shortened the fermentation time, resulting in increased <i>Q<sub>P</sub></i> values. Based on molecular taxonomic analysis of the five isolates using sequence analysis of the D1/D2 domain and the ITS1 and ITS2 regions, SSJ01KKU is <i>S. cerevisiae</i>, whereas SCJ04KKU, SCJ07KKU, SCJ09KKU and SCJ14KKU are <i>Pichia caribbica</i>.https://www.mdpi.com/1996-1073/14/3/557bioethanolhigh gravity fermentation<i>Saccharomyces cerevisiae</i>very high gravity fermentation |
| spellingShingle | Lakkana Laopaiboon Suntaree Suporn Preekamol Klanrit Niphaphat Phukoetphim Chalida Daengbussadee Pattana Laopaiboon Novel Effective Yeast Strains and Their Performance in High Gravity and Very High Gravity Ethanol Fermentations from Sweet Sorghum Juice Energies bioethanol high gravity fermentation <i>Saccharomyces cerevisiae</i> very high gravity fermentation |
| title | Novel Effective Yeast Strains and Their Performance in High Gravity and Very High Gravity Ethanol Fermentations from Sweet Sorghum Juice |
| title_full | Novel Effective Yeast Strains and Their Performance in High Gravity and Very High Gravity Ethanol Fermentations from Sweet Sorghum Juice |
| title_fullStr | Novel Effective Yeast Strains and Their Performance in High Gravity and Very High Gravity Ethanol Fermentations from Sweet Sorghum Juice |
| title_full_unstemmed | Novel Effective Yeast Strains and Their Performance in High Gravity and Very High Gravity Ethanol Fermentations from Sweet Sorghum Juice |
| title_short | Novel Effective Yeast Strains and Their Performance in High Gravity and Very High Gravity Ethanol Fermentations from Sweet Sorghum Juice |
| title_sort | novel effective yeast strains and their performance in high gravity and very high gravity ethanol fermentations from sweet sorghum juice |
| topic | bioethanol high gravity fermentation <i>Saccharomyces cerevisiae</i> very high gravity fermentation |
| url | https://www.mdpi.com/1996-1073/14/3/557 |
| work_keys_str_mv | AT lakkanalaopaiboon noveleffectiveyeaststrainsandtheirperformanceinhighgravityandveryhighgravityethanolfermentationsfromsweetsorghumjuice AT suntareesuporn noveleffectiveyeaststrainsandtheirperformanceinhighgravityandveryhighgravityethanolfermentationsfromsweetsorghumjuice AT preekamolklanrit noveleffectiveyeaststrainsandtheirperformanceinhighgravityandveryhighgravityethanolfermentationsfromsweetsorghumjuice AT niphaphatphukoetphim noveleffectiveyeaststrainsandtheirperformanceinhighgravityandveryhighgravityethanolfermentationsfromsweetsorghumjuice AT chalidadaengbussadee noveleffectiveyeaststrainsandtheirperformanceinhighgravityandveryhighgravityethanolfermentationsfromsweetsorghumjuice AT pattanalaopaiboon noveleffectiveyeaststrainsandtheirperformanceinhighgravityandveryhighgravityethanolfermentationsfromsweetsorghumjuice |