Oxidized low-density lipoprotein changes the inflammatory status and metabolomics profiles in human and mouse macrophages and microglia
The conjunctiva of primary open angle glaucoma patients showed high level of oxidized low-density lipoprotein (ox-LDL), which is associated with the inflammatory response. Microglia and macrophages are the immune cells involved in retinal ganglion cell survival regulation; yet, their roles of the ox...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-04-01
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| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024048370 |
| _version_ | 1797220264974483456 |
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| author | Yaru Sun Jia-Jian Liang Jianming Xu Kewen Zhou Changzhen Fu Shao-Lang Chen Rucui Yang Tsz Kin Ng Qingping Liu Mingzhi Zhang |
| author_facet | Yaru Sun Jia-Jian Liang Jianming Xu Kewen Zhou Changzhen Fu Shao-Lang Chen Rucui Yang Tsz Kin Ng Qingping Liu Mingzhi Zhang |
| author_sort | Yaru Sun |
| collection | DOAJ |
| description | The conjunctiva of primary open angle glaucoma patients showed high level of oxidized low-density lipoprotein (ox-LDL), which is associated with the inflammatory response. Microglia and macrophages are the immune cells involved in retinal ganglion cell survival regulation; yet, their roles of the ox-LDL-induced inflammation in glaucoma remain elusive. Here we aimed to investigate the lipid uptake, inflammatory cytokine expression, and metabolomics profiles of human and murine-derived microglial and macrophage cell lines treated with ox-LDL. Under the same ox-LDL concentration, macrophages exhibited higher lipid uptake and expression of pro-inflammatory cytokines as compared to microglia. The ox-LDL increased the levels of fatty acid metabolites in macrophages and sphingomyelin metabolites in microglia. In summary, this study revealed the heterogeneity in the inflammatory capacity and metabolic profiles of macrophages and microglia under the stimulation of ox-LDL. |
| first_indexed | 2024-04-24T12:46:47Z |
| format | Article |
| id | doaj.art-65f4a3f32a004bec9cc5592a344d155d |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-24T12:46:47Z |
| publishDate | 2024-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-65f4a3f32a004bec9cc5592a344d155d2024-04-07T04:35:53ZengElsevierHeliyon2405-84402024-04-01107e28806Oxidized low-density lipoprotein changes the inflammatory status and metabolomics profiles in human and mouse macrophages and microgliaYaru Sun0Jia-Jian Liang1Jianming Xu2Kewen Zhou3Changzhen Fu4Shao-Lang Chen5Rucui Yang6Tsz Kin Ng7Qingping Liu8Mingzhi Zhang9Joint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong, Shantou, Gaungdong, China; Shantou University Medical College, Shantou, Guangdong, ChinaJoint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong, Shantou, Gaungdong, ChinaJoint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong, Shantou, Gaungdong, China; Shantou University Medical College, Shantou, Guangdong, ChinaJoint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong, Shantou, Gaungdong, China; Shantou University Medical College, Shantou, Guangdong, ChinaJoint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong, Shantou, Gaungdong, ChinaJoint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong, Shantou, Gaungdong, ChinaJoint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong, Shantou, Gaungdong, China; Shantou University Medical College, Shantou, Guangdong, ChinaJoint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong, Shantou, Gaungdong, China; Department of Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, Hong KongJoint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong, Shantou, Gaungdong, China; Corresponding author.Joint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong, Shantou, Gaungdong, China; Corresponding author.The conjunctiva of primary open angle glaucoma patients showed high level of oxidized low-density lipoprotein (ox-LDL), which is associated with the inflammatory response. Microglia and macrophages are the immune cells involved in retinal ganglion cell survival regulation; yet, their roles of the ox-LDL-induced inflammation in glaucoma remain elusive. Here we aimed to investigate the lipid uptake, inflammatory cytokine expression, and metabolomics profiles of human and murine-derived microglial and macrophage cell lines treated with ox-LDL. Under the same ox-LDL concentration, macrophages exhibited higher lipid uptake and expression of pro-inflammatory cytokines as compared to microglia. The ox-LDL increased the levels of fatty acid metabolites in macrophages and sphingomyelin metabolites in microglia. In summary, this study revealed the heterogeneity in the inflammatory capacity and metabolic profiles of macrophages and microglia under the stimulation of ox-LDL.http://www.sciencedirect.com/science/article/pii/S2405844024048370Oxidized low-density lipoproteinMacrophagesMicrogliaInflammatory responseMetabolites |
| spellingShingle | Yaru Sun Jia-Jian Liang Jianming Xu Kewen Zhou Changzhen Fu Shao-Lang Chen Rucui Yang Tsz Kin Ng Qingping Liu Mingzhi Zhang Oxidized low-density lipoprotein changes the inflammatory status and metabolomics profiles in human and mouse macrophages and microglia Heliyon Oxidized low-density lipoprotein Macrophages Microglia Inflammatory response Metabolites |
| title | Oxidized low-density lipoprotein changes the inflammatory status and metabolomics profiles in human and mouse macrophages and microglia |
| title_full | Oxidized low-density lipoprotein changes the inflammatory status and metabolomics profiles in human and mouse macrophages and microglia |
| title_fullStr | Oxidized low-density lipoprotein changes the inflammatory status and metabolomics profiles in human and mouse macrophages and microglia |
| title_full_unstemmed | Oxidized low-density lipoprotein changes the inflammatory status and metabolomics profiles in human and mouse macrophages and microglia |
| title_short | Oxidized low-density lipoprotein changes the inflammatory status and metabolomics profiles in human and mouse macrophages and microglia |
| title_sort | oxidized low density lipoprotein changes the inflammatory status and metabolomics profiles in human and mouse macrophages and microglia |
| topic | Oxidized low-density lipoprotein Macrophages Microglia Inflammatory response Metabolites |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024048370 |
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