Isolation and Structural Characterization of Alginates from the Kelp Species <i>Laminaria ochroleuca</i> and <i>Saccorhiza polyschides</i> from the Atlantic Coast of Morocco
Alginates are linear unbranched polysaccharides produced by brown seaweeds. The gel properties of this phycocolloid are mainly linked to the monomer composition, sequential structure and molecular weight of the polymeric chain. Nevertheless, these structural parameters are also dependent on the age...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-09-01
|
| Series: | Colloids and Interfaces |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2504-5377/6/4/51 |
| _version_ | 1827641216938803200 |
|---|---|
| author | Soukaina Kaidi Fouad Bentiss Charafeddine Jama Khaoula Khaya Zahira Belattmania Abdeltif Reani Brahim Sabour |
| author_facet | Soukaina Kaidi Fouad Bentiss Charafeddine Jama Khaoula Khaya Zahira Belattmania Abdeltif Reani Brahim Sabour |
| author_sort | Soukaina Kaidi |
| collection | DOAJ |
| description | Alginates are linear unbranched polysaccharides produced by brown seaweeds. The gel properties of this phycocolloid are mainly linked to the monomer composition, sequential structure and molecular weight of the polymeric chain. Nevertheless, these structural parameters are also dependent on the age and tissue of the thalli used. In this work, the kelp species <i>Laminaria ochroleuca</i> and <i>Saccorhiza polyschides</i> harvested from the Moroccan coast were analyzed for their alginates content and composition in different thalli parts (blade and stipe). Spectroscopic characterization (<sup>1</sup>H NMR and FT-IR), viscosity, and molecular weight of the extracted alginates were investigated. The obtained results showed that the alginate contents ranged from 20.19 ± 2 to 49.8 ± 2.4% dw. FT-IR spectroscopy exhibited similar spectra between the alginates extracted from both species and the commercial alginate. The <sup>1</sup>H-NMR results revealed interesting variations in block composition between species, and less disparity with regard to the tissue type (M/G values ranging from 0.89 to 2.14). High mannuronate content was found in all alginates except for those extracted from the blade of <i>L. ochroleuca</i>. The homopolymeric fractions F<sub>MM</sub> are remarkably high compared to the F<sub>GG</sub> and heteropolymeric fractions (F<sub>GM</sub>/F<sub>MG</sub>) in <i>S. polyschides</i>. However, for <i>L. ochroleuca</i>, the heteropolymeric fractions are quite abundant, accounting for more than 34% of the polymer diads. The alginates extracted from both species indicated low values for the intrinsic viscosity. Based on the yield and the structural properties of their alginates, this study suggests that both <i>L. ochroleuca</i> and <i>S.polyschides</i> could be considered potential alginophytes to be incorporated into the industry of alginates. It also proposes an optimization of the yield, using the different thalli parts in the extraction (blade and stipe). The chemical structure and viscosity of their alginates may contribute to expanding their applications, especially in biotechnology and medical fields. |
| first_indexed | 2024-03-09T17:10:17Z |
| format | Article |
| id | doaj.art-65f95b3a4554494bb9fd2445638d530c |
| institution | Directory Open Access Journal |
| issn | 2504-5377 |
| language | English |
| last_indexed | 2024-03-09T17:10:17Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Colloids and Interfaces |
| spelling | doaj.art-65f95b3a4554494bb9fd2445638d530c2023-11-24T14:06:24ZengMDPI AGColloids and Interfaces2504-53772022-09-01645110.3390/colloids6040051Isolation and Structural Characterization of Alginates from the Kelp Species <i>Laminaria ochroleuca</i> and <i>Saccorhiza polyschides</i> from the Atlantic Coast of MoroccoSoukaina Kaidi0Fouad Bentiss1Charafeddine Jama2Khaoula Khaya3Zahira Belattmania4Abdeltif Reani5Brahim Sabour6Phycology, Blue Biodiversity & Biotechnology R.U., Laboratory of Plant Biotechnology, Ecology and Ecosystem Valorization, URL–CNRST n°10, Faculty of Sciences, University Chouaib Doukkali, P.O. Box 20, El Jadida M-24000, MoroccoLaboratory of Catalysis and Corrosion of Materials, Faculty of Sciences, University Chouaib Doukkali, P.O. Box 20, El Jadida M-24000, MoroccoMaterials and Transformations Unit, University of Lille, CNRS, INRAE, Centrale Lille, UMR 8207-UMET, F-59000 Lille, FrancePhycology, Blue Biodiversity & Biotechnology R.U., Laboratory of Plant Biotechnology, Ecology and Ecosystem Valorization, URL–CNRST n°10, Faculty of Sciences, University Chouaib Doukkali, P.O. Box 20, El Jadida M-24000, MoroccoPhycology, Blue Biodiversity & Biotechnology R.U., Laboratory of Plant Biotechnology, Ecology and Ecosystem Valorization, URL–CNRST n°10, Faculty of Sciences, University Chouaib Doukkali, P.O. Box 20, El Jadida M-24000, MoroccoPhycology, Blue Biodiversity & Biotechnology R.U., Laboratory of Plant Biotechnology, Ecology and Ecosystem Valorization, URL–CNRST n°10, Faculty of Sciences, University Chouaib Doukkali, P.O. Box 20, El Jadida M-24000, MoroccoPhycology, Blue Biodiversity & Biotechnology R.U., Laboratory of Plant Biotechnology, Ecology and Ecosystem Valorization, URL–CNRST n°10, Faculty of Sciences, University Chouaib Doukkali, P.O. Box 20, El Jadida M-24000, MoroccoAlginates are linear unbranched polysaccharides produced by brown seaweeds. The gel properties of this phycocolloid are mainly linked to the monomer composition, sequential structure and molecular weight of the polymeric chain. Nevertheless, these structural parameters are also dependent on the age and tissue of the thalli used. In this work, the kelp species <i>Laminaria ochroleuca</i> and <i>Saccorhiza polyschides</i> harvested from the Moroccan coast were analyzed for their alginates content and composition in different thalli parts (blade and stipe). Spectroscopic characterization (<sup>1</sup>H NMR and FT-IR), viscosity, and molecular weight of the extracted alginates were investigated. The obtained results showed that the alginate contents ranged from 20.19 ± 2 to 49.8 ± 2.4% dw. FT-IR spectroscopy exhibited similar spectra between the alginates extracted from both species and the commercial alginate. The <sup>1</sup>H-NMR results revealed interesting variations in block composition between species, and less disparity with regard to the tissue type (M/G values ranging from 0.89 to 2.14). High mannuronate content was found in all alginates except for those extracted from the blade of <i>L. ochroleuca</i>. The homopolymeric fractions F<sub>MM</sub> are remarkably high compared to the F<sub>GG</sub> and heteropolymeric fractions (F<sub>GM</sub>/F<sub>MG</sub>) in <i>S. polyschides</i>. However, for <i>L. ochroleuca</i>, the heteropolymeric fractions are quite abundant, accounting for more than 34% of the polymer diads. The alginates extracted from both species indicated low values for the intrinsic viscosity. Based on the yield and the structural properties of their alginates, this study suggests that both <i>L. ochroleuca</i> and <i>S.polyschides</i> could be considered potential alginophytes to be incorporated into the industry of alginates. It also proposes an optimization of the yield, using the different thalli parts in the extraction (blade and stipe). The chemical structure and viscosity of their alginates may contribute to expanding their applications, especially in biotechnology and medical fields.https://www.mdpi.com/2504-5377/6/4/51kelps<i>Laminaria ochroleuca</i><i>Saccorhiza polyschides</i>alginates<sup>1</sup>H NMRFT-IR |
| spellingShingle | Soukaina Kaidi Fouad Bentiss Charafeddine Jama Khaoula Khaya Zahira Belattmania Abdeltif Reani Brahim Sabour Isolation and Structural Characterization of Alginates from the Kelp Species <i>Laminaria ochroleuca</i> and <i>Saccorhiza polyschides</i> from the Atlantic Coast of Morocco Colloids and Interfaces kelps <i>Laminaria ochroleuca</i> <i>Saccorhiza polyschides</i> alginates <sup>1</sup>H NMR FT-IR |
| title | Isolation and Structural Characterization of Alginates from the Kelp Species <i>Laminaria ochroleuca</i> and <i>Saccorhiza polyschides</i> from the Atlantic Coast of Morocco |
| title_full | Isolation and Structural Characterization of Alginates from the Kelp Species <i>Laminaria ochroleuca</i> and <i>Saccorhiza polyschides</i> from the Atlantic Coast of Morocco |
| title_fullStr | Isolation and Structural Characterization of Alginates from the Kelp Species <i>Laminaria ochroleuca</i> and <i>Saccorhiza polyschides</i> from the Atlantic Coast of Morocco |
| title_full_unstemmed | Isolation and Structural Characterization of Alginates from the Kelp Species <i>Laminaria ochroleuca</i> and <i>Saccorhiza polyschides</i> from the Atlantic Coast of Morocco |
| title_short | Isolation and Structural Characterization of Alginates from the Kelp Species <i>Laminaria ochroleuca</i> and <i>Saccorhiza polyschides</i> from the Atlantic Coast of Morocco |
| title_sort | isolation and structural characterization of alginates from the kelp species i laminaria ochroleuca i and i saccorhiza polyschides i from the atlantic coast of morocco |
| topic | kelps <i>Laminaria ochroleuca</i> <i>Saccorhiza polyschides</i> alginates <sup>1</sup>H NMR FT-IR |
| url | https://www.mdpi.com/2504-5377/6/4/51 |
| work_keys_str_mv | AT soukainakaidi isolationandstructuralcharacterizationofalginatesfromthekelpspeciesilaminariaochroleucaiandisaccorhizapolyschidesifromtheatlanticcoastofmorocco AT fouadbentiss isolationandstructuralcharacterizationofalginatesfromthekelpspeciesilaminariaochroleucaiandisaccorhizapolyschidesifromtheatlanticcoastofmorocco AT charafeddinejama isolationandstructuralcharacterizationofalginatesfromthekelpspeciesilaminariaochroleucaiandisaccorhizapolyschidesifromtheatlanticcoastofmorocco AT khaoulakhaya isolationandstructuralcharacterizationofalginatesfromthekelpspeciesilaminariaochroleucaiandisaccorhizapolyschidesifromtheatlanticcoastofmorocco AT zahirabelattmania isolationandstructuralcharacterizationofalginatesfromthekelpspeciesilaminariaochroleucaiandisaccorhizapolyschidesifromtheatlanticcoastofmorocco AT abdeltifreani isolationandstructuralcharacterizationofalginatesfromthekelpspeciesilaminariaochroleucaiandisaccorhizapolyschidesifromtheatlanticcoastofmorocco AT brahimsabour isolationandstructuralcharacterizationofalginatesfromthekelpspeciesilaminariaochroleucaiandisaccorhizapolyschidesifromtheatlanticcoastofmorocco |