Graphene oxide/ε-poly-L-lysine self-assembled functionalized coatings improve the biocompatibility and antibacterial properties of titanium implants
The construction of an antibacterial biological coating on titanium surface plays an important role in the long-term stability of oral implant restoration. Graphene oxide (GO) has been widely studied because of its excellent antibacterial properties and osteogenic activity. However, striking a balan...
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| Format: | Article |
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Frontiers Media S.A.
2024-04-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2024.1381685/full |
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| author | Xiaoxiao You Xiaoxiao You Xiaoxiao You Xiaoxiao You Xiaoxiao You Zhongke Wang Zhongke Wang Zhongke Wang Zhongke Wang Li Wang Li Wang Li Wang Li Wang Youbo Liu Youbo Liu Youbo Liu Youbo Liu Hongmei Chen Xiaorong Lan Xiaorong Lan Xiaorong Lan Ling Guo Ling Guo Ling Guo Ling Guo |
| author_facet | Xiaoxiao You Xiaoxiao You Xiaoxiao You Xiaoxiao You Xiaoxiao You Zhongke Wang Zhongke Wang Zhongke Wang Zhongke Wang Li Wang Li Wang Li Wang Li Wang Youbo Liu Youbo Liu Youbo Liu Youbo Liu Hongmei Chen Xiaorong Lan Xiaorong Lan Xiaorong Lan Ling Guo Ling Guo Ling Guo Ling Guo |
| author_sort | Xiaoxiao You |
| collection | DOAJ |
| description | The construction of an antibacterial biological coating on titanium surface plays an important role in the long-term stability of oral implant restoration. Graphene oxide (GO) has been widely studied because of its excellent antibacterial properties and osteogenic activity. However, striking a balance between its biological toxicity and antibacterial properties remains a significant challenge with GO. ε-poly-L-lysine (PLL) has broad-spectrum antibacterial activity and ultra-high safety performance. Using Layer-by-layer self-assembly technology (LBL), different layers of PLL/GO coatings and GO self-assembly coatings were assembled on the surface of titanium sheet. The materials were characterized using scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS) and contact angle test. The antibacterial properties of Porphyromonas gingivalis (P.g.) were analyzed through SEM, coated plate experiment, and inhibition zone experiment. CCK-8 was used to determine the cytotoxicity of the material to MC3T3 cells, and zebrafish larvae and embryos were used to determine the developmental toxicity and inflammatory effects of the material. The results show that the combined assembly of 20 layers of GO and PLL exhibits good antibacterial properties and no biological toxicity, suggesting a potential application for a titanium-based implant modification scheme. |
| first_indexed | 2024-04-24T13:51:28Z |
| format | Article |
| id | doaj.art-65fb16be658d4ea182da1a15ab5d509d |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-04-24T13:51:28Z |
| publishDate | 2024-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-65fb16be658d4ea182da1a15ab5d509d2024-04-04T04:40:46ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852024-04-011210.3389/fbioe.2024.13816851381685Graphene oxide/ε-poly-L-lysine self-assembled functionalized coatings improve the biocompatibility and antibacterial properties of titanium implantsXiaoxiao You0Xiaoxiao You1Xiaoxiao You2Xiaoxiao You3Xiaoxiao You4Zhongke Wang5Zhongke Wang6Zhongke Wang7Zhongke Wang8Li Wang9Li Wang10Li Wang11Li Wang12Youbo Liu13Youbo Liu14Youbo Liu15Youbo Liu16Hongmei Chen17Xiaorong Lan18Xiaorong Lan19Xiaorong Lan20Ling Guo21Ling Guo22Ling Guo23Ling Guo24Department of Oral Prosthodontics, The Affiliated Stomatological Hospital, Southwest Medical University, Luzhou, ChinaInstitute of Stomatology, Southwest Medical University, Luzhou, ChinaSchool of Stomatology, Southwest Medical University, Luzhou, ChinaLuzhou Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Luzhou, ChinaThe Public Platform of Zebrafish Technology, Public Center of Experimental Technology, Southwest Medical University, Luzhou, ChinaDepartment of Oral Prosthodontics, The Affiliated Stomatological Hospital, Southwest Medical University, Luzhou, ChinaInstitute of Stomatology, Southwest Medical University, Luzhou, ChinaSchool of Stomatology, Southwest Medical University, Luzhou, ChinaLuzhou Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Luzhou, ChinaDepartment of Oral Prosthodontics, The Affiliated Stomatological Hospital, Southwest Medical University, Luzhou, ChinaInstitute of Stomatology, Southwest Medical University, Luzhou, ChinaSchool of Stomatology, Southwest Medical University, Luzhou, ChinaLuzhou Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Luzhou, ChinaDepartment of Oral Prosthodontics, The Affiliated Stomatological Hospital, Southwest Medical University, Luzhou, ChinaInstitute of Stomatology, Southwest Medical University, Luzhou, ChinaSchool of Stomatology, Southwest Medical University, Luzhou, ChinaLuzhou Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Luzhou, ChinaDepartment of Oral Prosthodontics, The Affiliated Stomatological Hospital, Southwest Medical University, Luzhou, ChinaInstitute of Stomatology, Southwest Medical University, Luzhou, ChinaSchool of Stomatology, Southwest Medical University, Luzhou, ChinaLuzhou Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Luzhou, ChinaDepartment of Oral Prosthodontics, The Affiliated Stomatological Hospital, Southwest Medical University, Luzhou, ChinaInstitute of Stomatology, Southwest Medical University, Luzhou, ChinaSchool of Stomatology, Southwest Medical University, Luzhou, ChinaLuzhou Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Luzhou, ChinaThe construction of an antibacterial biological coating on titanium surface plays an important role in the long-term stability of oral implant restoration. Graphene oxide (GO) has been widely studied because of its excellent antibacterial properties and osteogenic activity. However, striking a balance between its biological toxicity and antibacterial properties remains a significant challenge with GO. ε-poly-L-lysine (PLL) has broad-spectrum antibacterial activity and ultra-high safety performance. Using Layer-by-layer self-assembly technology (LBL), different layers of PLL/GO coatings and GO self-assembly coatings were assembled on the surface of titanium sheet. The materials were characterized using scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS) and contact angle test. The antibacterial properties of Porphyromonas gingivalis (P.g.) were analyzed through SEM, coated plate experiment, and inhibition zone experiment. CCK-8 was used to determine the cytotoxicity of the material to MC3T3 cells, and zebrafish larvae and embryos were used to determine the developmental toxicity and inflammatory effects of the material. The results show that the combined assembly of 20 layers of GO and PLL exhibits good antibacterial properties and no biological toxicity, suggesting a potential application for a titanium-based implant modification scheme.https://www.frontiersin.org/articles/10.3389/fbioe.2024.1381685/fullgraphene oxideε-poly-L-lysineantibacterialbiocompatibilitylayer-by-layer self-assembly |
| spellingShingle | Xiaoxiao You Xiaoxiao You Xiaoxiao You Xiaoxiao You Xiaoxiao You Zhongke Wang Zhongke Wang Zhongke Wang Zhongke Wang Li Wang Li Wang Li Wang Li Wang Youbo Liu Youbo Liu Youbo Liu Youbo Liu Hongmei Chen Xiaorong Lan Xiaorong Lan Xiaorong Lan Ling Guo Ling Guo Ling Guo Ling Guo Graphene oxide/ε-poly-L-lysine self-assembled functionalized coatings improve the biocompatibility and antibacterial properties of titanium implants Frontiers in Bioengineering and Biotechnology graphene oxide ε-poly-L-lysine antibacterial biocompatibility layer-by-layer self-assembly |
| title | Graphene oxide/ε-poly-L-lysine self-assembled functionalized coatings improve the biocompatibility and antibacterial properties of titanium implants |
| title_full | Graphene oxide/ε-poly-L-lysine self-assembled functionalized coatings improve the biocompatibility and antibacterial properties of titanium implants |
| title_fullStr | Graphene oxide/ε-poly-L-lysine self-assembled functionalized coatings improve the biocompatibility and antibacterial properties of titanium implants |
| title_full_unstemmed | Graphene oxide/ε-poly-L-lysine self-assembled functionalized coatings improve the biocompatibility and antibacterial properties of titanium implants |
| title_short | Graphene oxide/ε-poly-L-lysine self-assembled functionalized coatings improve the biocompatibility and antibacterial properties of titanium implants |
| title_sort | graphene oxide ε poly l lysine self assembled functionalized coatings improve the biocompatibility and antibacterial properties of titanium implants |
| topic | graphene oxide ε-poly-L-lysine antibacterial biocompatibility layer-by-layer self-assembly |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2024.1381685/full |
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