Prognostic and predictive value of circulating tumor cell analysis in colorectal cancer patients
<p>Abstract</p> <p>Objective</p> <p>The aim of this study was to assess the prognostic and predictive values of circulating tumor cell (CTC) analysis in colorectal cancer patients.</p> <p>Patients and methods</p> <p>Presence of CTCs was evaluated...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2012-11-01
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| Series: | Journal of Translational Medicine |
| Subjects: | |
| Online Access: | http://www.translational-medicine.com/content/10/1/222 |
| _version_ | 1819086911759712256 |
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| author | de Albuquerque Andreia Kubisch Ilja Stölzel Ulrich Ernst Dominikus Boese-Landgraf Joachim Breier Georg Stamminger Gudrun Fersis Nikos Kaul Sepp |
| author_facet | de Albuquerque Andreia Kubisch Ilja Stölzel Ulrich Ernst Dominikus Boese-Landgraf Joachim Breier Georg Stamminger Gudrun Fersis Nikos Kaul Sepp |
| author_sort | de Albuquerque Andreia |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Objective</p> <p>The aim of this study was to assess the prognostic and predictive values of circulating tumor cell (CTC) analysis in colorectal cancer patients.</p> <p>Patients and methods</p> <p>Presence of CTCs was evaluated in 60 colorectal cancer patients before systemic therapy - from which 33 patients were also evaluable for CTC analysis during the first 3 months of treatment - through immunomagnetic enrichment, using the antibodies BM7 and VU1D9 (targeting mucin 1 and EpCAM, respectively), followed by real-time RT-PCR analysis of the tumor-associated genes <it>KRT19</it>, <it>MUC1</it>, <it>EPCAM</it>, CEACAM5 and <it>BIRC5</it>.</p> <p>Results</p> <p>Patients were stratified into groups according to CTC detection (CTC negative, when all marker genes were negative; and CTC positive when at least one of the marker genes was positive). Patients with CTC positivity at baseline had a significant shorter median progression-free survival (median PFS 181.0 days; 95% CI 146.9-215.1) compared with patients with no CTCs (median PFS 329.0 days; 95% CI 299.6-358.4; Log-rank <it>P</it> < .0001). Moreover, a statistically significant correlation was also founded between CTC detection during treatment and radiographic findings at the 6 month staging. This correlation applied to CTC results before therapy (odds ratio (OR), 6.22), 1 to 4 weeks after beginning of treatment (OR, 5.50), 5 to 8 weeks after beginning of treatment (OR, 7.94) 9 to 12 weeks after beginning of treatment (OR, 14.00) and overall CTC fluctuation during the course of treatment (OR, 20.57).</p> <p>Conclusion</p> <p>The present study provides evidence of a strong correlation between CTC detection and radiographic disease progression in patients receiving chemotherapy for colorectal cancer. Our results suggest that in addition to the current prognostic factors, CTC analysis represent a potential complementary tool for prediction of colorectal cancer patients’ outcome. Moreover, the present test allows for molecular characterization of CTCs, which may be of relevance to the creation of personalized therapies.</p> |
| first_indexed | 2024-12-21T21:27:47Z |
| format | Article |
| id | doaj.art-65fb5f02a17e4d519e509bf1f307486b |
| institution | Directory Open Access Journal |
| issn | 1479-5876 |
| language | English |
| last_indexed | 2024-12-21T21:27:47Z |
| publishDate | 2012-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Translational Medicine |
| spelling | doaj.art-65fb5f02a17e4d519e509bf1f307486b2022-12-21T18:49:43ZengBMCJournal of Translational Medicine1479-58762012-11-0110122210.1186/1479-5876-10-222Prognostic and predictive value of circulating tumor cell analysis in colorectal cancer patientsde Albuquerque AndreiaKubisch IljaStölzel UlrichErnst DominikusBoese-Landgraf JoachimBreier GeorgStamminger GudrunFersis NikosKaul Sepp<p>Abstract</p> <p>Objective</p> <p>The aim of this study was to assess the prognostic and predictive values of circulating tumor cell (CTC) analysis in colorectal cancer patients.</p> <p>Patients and methods</p> <p>Presence of CTCs was evaluated in 60 colorectal cancer patients before systemic therapy - from which 33 patients were also evaluable for CTC analysis during the first 3 months of treatment - through immunomagnetic enrichment, using the antibodies BM7 and VU1D9 (targeting mucin 1 and EpCAM, respectively), followed by real-time RT-PCR analysis of the tumor-associated genes <it>KRT19</it>, <it>MUC1</it>, <it>EPCAM</it>, CEACAM5 and <it>BIRC5</it>.</p> <p>Results</p> <p>Patients were stratified into groups according to CTC detection (CTC negative, when all marker genes were negative; and CTC positive when at least one of the marker genes was positive). Patients with CTC positivity at baseline had a significant shorter median progression-free survival (median PFS 181.0 days; 95% CI 146.9-215.1) compared with patients with no CTCs (median PFS 329.0 days; 95% CI 299.6-358.4; Log-rank <it>P</it> < .0001). Moreover, a statistically significant correlation was also founded between CTC detection during treatment and radiographic findings at the 6 month staging. This correlation applied to CTC results before therapy (odds ratio (OR), 6.22), 1 to 4 weeks after beginning of treatment (OR, 5.50), 5 to 8 weeks after beginning of treatment (OR, 7.94) 9 to 12 weeks after beginning of treatment (OR, 14.00) and overall CTC fluctuation during the course of treatment (OR, 20.57).</p> <p>Conclusion</p> <p>The present study provides evidence of a strong correlation between CTC detection and radiographic disease progression in patients receiving chemotherapy for colorectal cancer. Our results suggest that in addition to the current prognostic factors, CTC analysis represent a potential complementary tool for prediction of colorectal cancer patients’ outcome. Moreover, the present test allows for molecular characterization of CTCs, which may be of relevance to the creation of personalized therapies.</p>http://www.translational-medicine.com/content/10/1/222Circulating tumor cellsColorectal cancerReverse transcription real-time polymerase chain reaction |
| spellingShingle | de Albuquerque Andreia Kubisch Ilja Stölzel Ulrich Ernst Dominikus Boese-Landgraf Joachim Breier Georg Stamminger Gudrun Fersis Nikos Kaul Sepp Prognostic and predictive value of circulating tumor cell analysis in colorectal cancer patients Journal of Translational Medicine Circulating tumor cells Colorectal cancer Reverse transcription real-time polymerase chain reaction |
| title | Prognostic and predictive value of circulating tumor cell analysis in colorectal cancer patients |
| title_full | Prognostic and predictive value of circulating tumor cell analysis in colorectal cancer patients |
| title_fullStr | Prognostic and predictive value of circulating tumor cell analysis in colorectal cancer patients |
| title_full_unstemmed | Prognostic and predictive value of circulating tumor cell analysis in colorectal cancer patients |
| title_short | Prognostic and predictive value of circulating tumor cell analysis in colorectal cancer patients |
| title_sort | prognostic and predictive value of circulating tumor cell analysis in colorectal cancer patients |
| topic | Circulating tumor cells Colorectal cancer Reverse transcription real-time polymerase chain reaction |
| url | http://www.translational-medicine.com/content/10/1/222 |
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