Electroacupuncture Alleviate Lung Injury of Sepsis Through α7nAChR and NF-κB Signaling Pathway
Abstract Background Sepsis is the leading cause of death in hospitalized patients in the intensive care unit (ICU). Although substantial progress has been made in studies on the treatment of sepsis, the mortality rate remains extremely high. We have previously reported that electroacupuncture (EA) i...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Springer
2022-07-01
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| Series: | Intensive Care Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/s44231-022-00008-1 |
| _version_ | 1827770515092144128 |
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| author | Xingxing Shi Lixia Du Zhongmin Fan Binxiao Su Yu Chen Zongping Fang Xijing Zhang |
| author_facet | Xingxing Shi Lixia Du Zhongmin Fan Binxiao Su Yu Chen Zongping Fang Xijing Zhang |
| author_sort | Xingxing Shi |
| collection | DOAJ |
| description | Abstract Background Sepsis is the leading cause of death in hospitalized patients in the intensive care unit (ICU). Although substantial progress has been made in studies on the treatment of sepsis, the mortality rate remains extremely high. We have previously reported that electroacupuncture (EA) induced tolerance against sepsis, but the underlying mechanism remains unclear. Methods C57BL/6 mice were pretreated with EA before sepsis was induced by cecal ligation and puncture (CLP). Then the indexes associated with pulmonary edema and mortality were tested. And the changes of endogenous cholinergic anti-inflammatory pathway especially their typical receptor α7nAChR were detected. Finally, the mechanism of EA in sepsis was explored through regulating the expression of α7nAChR. Results The expression of α7nAChR was significantly decreased after sepsis, while EA prevented this reduction. Methyllycaconitine (MLA), an antagonist of α7nAChR, attenuated the beneficial effects of EA. On the other hand, as an α7nAChR agonist, GTS-21 produced similar protective effects against sepsis. Furthermore, the EA-induced enhancement of α7nAChR and inhibition of NF-κB expression in the lungs were reversed by MLA administration. Conclusions EA robustly protects the lungs against sepsis and inhibits NF-κB release by activating α7nAChR in mice. |
| first_indexed | 2024-03-11T12:40:40Z |
| format | Article |
| id | doaj.art-6602d2a72e3847a8954caa1afd9a0b9f |
| institution | Directory Open Access Journal |
| issn | 2666-9862 |
| language | English |
| last_indexed | 2024-03-11T12:40:40Z |
| publishDate | 2022-07-01 |
| publisher | Springer |
| record_format | Article |
| series | Intensive Care Research |
| spelling | doaj.art-6602d2a72e3847a8954caa1afd9a0b9f2023-11-05T12:19:11ZengSpringerIntensive Care Research2666-98622022-07-0121-2344310.1007/s44231-022-00008-1Electroacupuncture Alleviate Lung Injury of Sepsis Through α7nAChR and NF-κB Signaling PathwayXingxing Shi0Lixia Du1Zhongmin Fan2Binxiao Su3Yu Chen4Zongping Fang5Xijing Zhang6Department of Critical Care Medicine and Department of Anesthesiology Perioperative Medicine, Xijing Hospital, Fourth Military Medical UniversityDepartment of Critical Care Medicine and Department of Anesthesiology Perioperative Medicine, Xijing Hospital, Fourth Military Medical UniversityDepartment of Critical Care Medicine and Department of Anesthesiology Perioperative Medicine, Xijing Hospital, Fourth Military Medical UniversityDepartment of Critical Care Medicine and Department of Anesthesiology Perioperative Medicine, Xijing Hospital, Fourth Military Medical UniversityDepartment of Critical Care Medicine and Department of Anesthesiology Perioperative Medicine, Xijing Hospital, Fourth Military Medical UniversityDepartment of Critical Care Medicine and Department of Anesthesiology Perioperative Medicine, Xijing Hospital, Fourth Military Medical UniversityDepartment of Critical Care Medicine and Department of Anesthesiology Perioperative Medicine, Xijing Hospital, Fourth Military Medical UniversityAbstract Background Sepsis is the leading cause of death in hospitalized patients in the intensive care unit (ICU). Although substantial progress has been made in studies on the treatment of sepsis, the mortality rate remains extremely high. We have previously reported that electroacupuncture (EA) induced tolerance against sepsis, but the underlying mechanism remains unclear. Methods C57BL/6 mice were pretreated with EA before sepsis was induced by cecal ligation and puncture (CLP). Then the indexes associated with pulmonary edema and mortality were tested. And the changes of endogenous cholinergic anti-inflammatory pathway especially their typical receptor α7nAChR were detected. Finally, the mechanism of EA in sepsis was explored through regulating the expression of α7nAChR. Results The expression of α7nAChR was significantly decreased after sepsis, while EA prevented this reduction. Methyllycaconitine (MLA), an antagonist of α7nAChR, attenuated the beneficial effects of EA. On the other hand, as an α7nAChR agonist, GTS-21 produced similar protective effects against sepsis. Furthermore, the EA-induced enhancement of α7nAChR and inhibition of NF-κB expression in the lungs were reversed by MLA administration. Conclusions EA robustly protects the lungs against sepsis and inhibits NF-κB release by activating α7nAChR in mice.https://doi.org/10.1007/s44231-022-00008-1SepsisElectroacupunctureCholinergic anti-inflammatory pathwayα7 nicotinic acetylcholine receptorNF-κB |
| spellingShingle | Xingxing Shi Lixia Du Zhongmin Fan Binxiao Su Yu Chen Zongping Fang Xijing Zhang Electroacupuncture Alleviate Lung Injury of Sepsis Through α7nAChR and NF-κB Signaling Pathway Intensive Care Research Sepsis Electroacupuncture Cholinergic anti-inflammatory pathway α7 nicotinic acetylcholine receptor NF-κB |
| title | Electroacupuncture Alleviate Lung Injury of Sepsis Through α7nAChR and NF-κB Signaling Pathway |
| title_full | Electroacupuncture Alleviate Lung Injury of Sepsis Through α7nAChR and NF-κB Signaling Pathway |
| title_fullStr | Electroacupuncture Alleviate Lung Injury of Sepsis Through α7nAChR and NF-κB Signaling Pathway |
| title_full_unstemmed | Electroacupuncture Alleviate Lung Injury of Sepsis Through α7nAChR and NF-κB Signaling Pathway |
| title_short | Electroacupuncture Alleviate Lung Injury of Sepsis Through α7nAChR and NF-κB Signaling Pathway |
| title_sort | electroacupuncture alleviate lung injury of sepsis through α7nachr and nf κb signaling pathway |
| topic | Sepsis Electroacupuncture Cholinergic anti-inflammatory pathway α7 nicotinic acetylcholine receptor NF-κB |
| url | https://doi.org/10.1007/s44231-022-00008-1 |
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