Up-Regulation of hsa-miR-210 Promotes Venous Metastasis and Predicts Poor Prognosis in Hepatocellular Carcinoma
Objective: To investigate the potential biomarkers for venous metastasis of hepatocellular carcinoma (HCC), and briefly discuss their target genes and the signaling pathways they are involved in.Materials and Method: The dataset GSE6857 was downloaded from GEO. Significantly differentially expressed...
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Frontiers Media S.A.
2018-12-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fonc.2018.00569/full |
| _version_ | 1818152299967545344 |
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| author | Jia Ji Jia Ji Yuan Rong Chang-Liang Luo Shuo Li Xiang Jiang Hong Weng Hao Chen Wu-Wen Zhang Wen Xie Fu-Bing Wang |
| author_facet | Jia Ji Jia Ji Yuan Rong Chang-Liang Luo Shuo Li Xiang Jiang Hong Weng Hao Chen Wu-Wen Zhang Wen Xie Fu-Bing Wang |
| author_sort | Jia Ji |
| collection | DOAJ |
| description | Objective: To investigate the potential biomarkers for venous metastasis of hepatocellular carcinoma (HCC), and briefly discuss their target genes and the signaling pathways they are involved in.Materials and Method: The dataset GSE6857 was downloaded from GEO. Significantly differentially expressed miRNAs were identified using the R package “limma,” After that, the survival analysis was conducted to discover the significance of these up-regulated miRNAs for the prognosis of HCC patients. Additionally, miRNAs which were up-regulated in venous metastasis positive HCC tissues and were significant for the prognosis of HCC patients were further verified in clinical samples using RT-qPCR. The miRNAs were then analyzed for their correlations with clinical characteristics including survival time, AFP level, pathological grade, TNM stage, tumor stage, lymph-node metastasis, distant metastasis, child-pugh score, vascular invasion, liver fibrosis and race using 375 HCC samples downloaded from the TCGA database. The target genes of these miRNAs were obtained using a miRNA target gene prediction database, and their functions were analyzed using the online tool DAVID.Results: 15 miRNAs were differentially expressed in samples with venous metastasis, among which 7 were up-regulated in venous metastasis positive HCC samples. As one of the up-regulated miRNAs, hsa-miR-210 was identified as an independent prognostic factor for HCC. Using RT-qPCR, it was evident that hsa-miR-210 expression was significantly higher in venous metastasis positive HCC samples (p = 0.0036). Further analysis indicated that hsa-miR-210 was positively associated with AFP level, pathological grade, TNM stage, tumor stage and vascular invasion. A total of 168 hsa-miR-210 target genes, which are mainly related to tumor metastasis and tumor signaling pathways, were also predicted in this study.Conclusion: hsa-miR-210 might promote vascular invasion of HCC cells and could be used as a prognostic biomarker. |
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| language | English |
| last_indexed | 2024-12-11T13:52:31Z |
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| series | Frontiers in Oncology |
| spelling | doaj.art-660457fbfbe64111b2eee44b632ca3ba2022-12-22T01:04:13ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2018-12-01810.3389/fonc.2018.00569414225Up-Regulation of hsa-miR-210 Promotes Venous Metastasis and Predicts Poor Prognosis in Hepatocellular CarcinomaJia Ji0Jia Ji1Yuan Rong2Chang-Liang Luo3Shuo Li4Xiang Jiang5Hong Weng6Hao Chen7Wu-Wen Zhang8Wen Xie9Fu-Bing Wang10Department of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan, ChinaDepartment of Laboratory Medicine, Wuhan Children's Hospital, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan, ChinaDepartment of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan, ChinaDepartment of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan, ChinaDepartment of Hepatobiliary and Pancreatic Surgery, Zhongnan Hospital of Wuhan University, Wuhan, ChinaCenter for Evidence-Based and Translational Medicine, Zhongnan Hospital of Wuhan University, Wuhan, ChinaDepartment of Pathology, Zhongnan Hospital of Wuhan University, Wuhan, ChinaDepartment of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan, ChinaDepartment of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan, ChinaDepartment of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan, ChinaObjective: To investigate the potential biomarkers for venous metastasis of hepatocellular carcinoma (HCC), and briefly discuss their target genes and the signaling pathways they are involved in.Materials and Method: The dataset GSE6857 was downloaded from GEO. Significantly differentially expressed miRNAs were identified using the R package “limma,” After that, the survival analysis was conducted to discover the significance of these up-regulated miRNAs for the prognosis of HCC patients. Additionally, miRNAs which were up-regulated in venous metastasis positive HCC tissues and were significant for the prognosis of HCC patients were further verified in clinical samples using RT-qPCR. The miRNAs were then analyzed for their correlations with clinical characteristics including survival time, AFP level, pathological grade, TNM stage, tumor stage, lymph-node metastasis, distant metastasis, child-pugh score, vascular invasion, liver fibrosis and race using 375 HCC samples downloaded from the TCGA database. The target genes of these miRNAs were obtained using a miRNA target gene prediction database, and their functions were analyzed using the online tool DAVID.Results: 15 miRNAs were differentially expressed in samples with venous metastasis, among which 7 were up-regulated in venous metastasis positive HCC samples. As one of the up-regulated miRNAs, hsa-miR-210 was identified as an independent prognostic factor for HCC. Using RT-qPCR, it was evident that hsa-miR-210 expression was significantly higher in venous metastasis positive HCC samples (p = 0.0036). Further analysis indicated that hsa-miR-210 was positively associated with AFP level, pathological grade, TNM stage, tumor stage and vascular invasion. A total of 168 hsa-miR-210 target genes, which are mainly related to tumor metastasis and tumor signaling pathways, were also predicted in this study.Conclusion: hsa-miR-210 might promote vascular invasion of HCC cells and could be used as a prognostic biomarker.https://www.frontiersin.org/article/10.3389/fonc.2018.00569/fullhsa-miR-210venous metastasisprognosishepatocellular carcinomabioinformatics analysisRT-qPCR |
| spellingShingle | Jia Ji Jia Ji Yuan Rong Chang-Liang Luo Shuo Li Xiang Jiang Hong Weng Hao Chen Wu-Wen Zhang Wen Xie Fu-Bing Wang Up-Regulation of hsa-miR-210 Promotes Venous Metastasis and Predicts Poor Prognosis in Hepatocellular Carcinoma Frontiers in Oncology hsa-miR-210 venous metastasis prognosis hepatocellular carcinoma bioinformatics analysis RT-qPCR |
| title | Up-Regulation of hsa-miR-210 Promotes Venous Metastasis and Predicts Poor Prognosis in Hepatocellular Carcinoma |
| title_full | Up-Regulation of hsa-miR-210 Promotes Venous Metastasis and Predicts Poor Prognosis in Hepatocellular Carcinoma |
| title_fullStr | Up-Regulation of hsa-miR-210 Promotes Venous Metastasis and Predicts Poor Prognosis in Hepatocellular Carcinoma |
| title_full_unstemmed | Up-Regulation of hsa-miR-210 Promotes Venous Metastasis and Predicts Poor Prognosis in Hepatocellular Carcinoma |
| title_short | Up-Regulation of hsa-miR-210 Promotes Venous Metastasis and Predicts Poor Prognosis in Hepatocellular Carcinoma |
| title_sort | up regulation of hsa mir 210 promotes venous metastasis and predicts poor prognosis in hepatocellular carcinoma |
| topic | hsa-miR-210 venous metastasis prognosis hepatocellular carcinoma bioinformatics analysis RT-qPCR |
| url | https://www.frontiersin.org/article/10.3389/fonc.2018.00569/full |
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