Quercetin attenuates lipopolysaccharide-induced hepatic inflammation by modulating autophagy and necroptosis
ABSTRACT: Lipopolysaccharide (LPS) from Gram-negative bacteria initially induces liver inflammation with proinflammatory cytokines expressions. However, the underlying hepatoprotective mechanism of quercetin on LPS-induced hepatic inflammation remains unclear. Specific pathogen-free chicken embryos...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-06-01
|
| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124003006 |
| _version_ | 1797213457528913920 |
|---|---|
| author | Jinhai Yu Rong Fu Amin Buhe Bing Xu |
| author_facet | Jinhai Yu Rong Fu Amin Buhe Bing Xu |
| author_sort | Jinhai Yu |
| collection | DOAJ |
| description | ABSTRACT: Lipopolysaccharide (LPS) from Gram-negative bacteria initially induces liver inflammation with proinflammatory cytokines expressions. However, the underlying hepatoprotective mechanism of quercetin on LPS-induced hepatic inflammation remains unclear. Specific pathogen-free chicken embryos (n = 120) were allocated control vehicle, PBS with or without ethanol vehicle, LPS (125 ng/egg) with or without quercetin treatment (10, 20, or 40 nmol/egg, respectively), quercetin groups (10, 20, or 40 nmol/egg). Fifteen-day-old embryonated eggs were inoculated abovementioned solutions via the allantoic cavity. At embryonic d 19, the livers of the embryos were collected for histopathological examination, RNA extraction, real-time polymerase chain reaction, and immunohistochemistry investigation. We found that the liver presented inflammatory response (heterophils infiltration) after LPS induction. The LPS-induced mRNA expressions of inflammation-related factors (TLR4, TNFα, IL-1β, IL-10, IL-6, MYD88, NF-κB1, p38, and MMP3) were upregulated after LPS induction when compared with the PBS group, while quercetin could downregulate these expressions as compared with the LPS group. Quercetin significantly decreased the immunopositivity to TLR4 and MMP3 in the treatment group when compared with the LPS group. Quercetin could significantly downregulate the mRNA expressions of autophagy-related genes (ATG5, ATG7, Beclin-1, LC3A, and LC3B) and necroptosis-related genes (Fas, Bcl-2, Drp1, and RIPK1) after LPS induction. Quercetin significantly decreased the immunopositivity to LC3 in the treatment group when compared with the LPS group; meanwhile, quercetin significantly decreased the protein expressions of LC3-I, LC3-II, and the rate of LC3-II/LC3-I. In conclusions, quercetin can alleviate hepatic inflammation induced by LPS through modulating autophagy and necroptosis. |
| first_indexed | 2024-04-24T10:58:35Z |
| format | Article |
| id | doaj.art-6609adcb42b04d949f7240d931a6964b |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-04-24T10:58:35Z |
| publishDate | 2024-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-6609adcb42b04d949f7240d931a6964b2024-04-12T04:44:15ZengElsevierPoultry Science0032-57912024-06-011036103719Quercetin attenuates lipopolysaccharide-induced hepatic inflammation by modulating autophagy and necroptosisJinhai Yu0Rong Fu1Amin Buhe2Bing Xu3Camellia Research Institute, The Innovation Institute of Agricultural Technology, Department of Life Science, Shangrao Normal University, Shangrao 334001, China; Corresponding author:Department of Literature and Media, Shangrao Normal University, Shangrao 334001, ChinaDepartment of Cancer Surgery, Beijing Shijitan Hospital Affiliated with Capital Medical University, Beijing 100038, ChinaCamellia Research Institute, The Innovation Institute of Agricultural Technology, Department of Life Science, Shangrao Normal University, Shangrao 334001, ChinaABSTRACT: Lipopolysaccharide (LPS) from Gram-negative bacteria initially induces liver inflammation with proinflammatory cytokines expressions. However, the underlying hepatoprotective mechanism of quercetin on LPS-induced hepatic inflammation remains unclear. Specific pathogen-free chicken embryos (n = 120) were allocated control vehicle, PBS with or without ethanol vehicle, LPS (125 ng/egg) with or without quercetin treatment (10, 20, or 40 nmol/egg, respectively), quercetin groups (10, 20, or 40 nmol/egg). Fifteen-day-old embryonated eggs were inoculated abovementioned solutions via the allantoic cavity. At embryonic d 19, the livers of the embryos were collected for histopathological examination, RNA extraction, real-time polymerase chain reaction, and immunohistochemistry investigation. We found that the liver presented inflammatory response (heterophils infiltration) after LPS induction. The LPS-induced mRNA expressions of inflammation-related factors (TLR4, TNFα, IL-1β, IL-10, IL-6, MYD88, NF-κB1, p38, and MMP3) were upregulated after LPS induction when compared with the PBS group, while quercetin could downregulate these expressions as compared with the LPS group. Quercetin significantly decreased the immunopositivity to TLR4 and MMP3 in the treatment group when compared with the LPS group. Quercetin could significantly downregulate the mRNA expressions of autophagy-related genes (ATG5, ATG7, Beclin-1, LC3A, and LC3B) and necroptosis-related genes (Fas, Bcl-2, Drp1, and RIPK1) after LPS induction. Quercetin significantly decreased the immunopositivity to LC3 in the treatment group when compared with the LPS group; meanwhile, quercetin significantly decreased the protein expressions of LC3-I, LC3-II, and the rate of LC3-II/LC3-I. In conclusions, quercetin can alleviate hepatic inflammation induced by LPS through modulating autophagy and necroptosis.http://www.sciencedirect.com/science/article/pii/S0032579124003006quercetinendotoxinliverinjuryprogrammed cell death |
| spellingShingle | Jinhai Yu Rong Fu Amin Buhe Bing Xu Quercetin attenuates lipopolysaccharide-induced hepatic inflammation by modulating autophagy and necroptosis Poultry Science quercetin endotoxin liver injury programmed cell death |
| title | Quercetin attenuates lipopolysaccharide-induced hepatic inflammation by modulating autophagy and necroptosis |
| title_full | Quercetin attenuates lipopolysaccharide-induced hepatic inflammation by modulating autophagy and necroptosis |
| title_fullStr | Quercetin attenuates lipopolysaccharide-induced hepatic inflammation by modulating autophagy and necroptosis |
| title_full_unstemmed | Quercetin attenuates lipopolysaccharide-induced hepatic inflammation by modulating autophagy and necroptosis |
| title_short | Quercetin attenuates lipopolysaccharide-induced hepatic inflammation by modulating autophagy and necroptosis |
| title_sort | quercetin attenuates lipopolysaccharide induced hepatic inflammation by modulating autophagy and necroptosis |
| topic | quercetin endotoxin liver injury programmed cell death |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124003006 |
| work_keys_str_mv | AT jinhaiyu quercetinattenuateslipopolysaccharideinducedhepaticinflammationbymodulatingautophagyandnecroptosis AT rongfu quercetinattenuateslipopolysaccharideinducedhepaticinflammationbymodulatingautophagyandnecroptosis AT aminbuhe quercetinattenuateslipopolysaccharideinducedhepaticinflammationbymodulatingautophagyandnecroptosis AT bingxu quercetinattenuateslipopolysaccharideinducedhepaticinflammationbymodulatingautophagyandnecroptosis |