Manufactured Nano-Objects Confer Viral Protection against Cucurbit Chlorotic Yellows Virus (CCYV) Infecting <i>Nicotiana benthamiana</i>
Nanotechnology has emerged as a new tool to combat phytopathogens in agricultural crops. Cucurbit chlorotic yellows virus (CCYV) mainly infects Solanaceae crops and causes significant crop losses. Nanomaterials (NMs) may have efficacy against plant viruses, but the mechanisms underlying complex nano...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/9/1837 |
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| author | Mayasar I. Al-Zaban Sadeq K. Alhag Anas S. Dablool Ahmed Ezzat Ahmed Saad Alghamdi Baber Ali Fatimah A. Al-Saeed Muhammad Hamzah Saleem Peter Poczai |
| author_facet | Mayasar I. Al-Zaban Sadeq K. Alhag Anas S. Dablool Ahmed Ezzat Ahmed Saad Alghamdi Baber Ali Fatimah A. Al-Saeed Muhammad Hamzah Saleem Peter Poczai |
| author_sort | Mayasar I. Al-Zaban |
| collection | DOAJ |
| description | Nanotechnology has emerged as a new tool to combat phytopathogens in agricultural crops. Cucurbit chlorotic yellows virus (CCYV) mainly infects Solanaceae crops and causes significant crop losses. Nanomaterials (NMs) may have efficacy against plant viruses, but the mechanisms underlying complex nanomaterials-plant-virus interactions remain elusive. We challenged <i>Nicotiana benthamiana</i> plants with GFP-tagged CCYV and observed morphological, physiological, and molecular changes in response to 21-d foliar exposure to nanoscale Fe and Zn and C<sub>60</sub> fullerenes at 100 mg/L concentration for 21 days. We observed that in response to C<sub>60</sub> (100 mg/L) treatment, plants displayed a normal phenotype while the viral infection was not seen until 5 days post-inoculation. On the contrary, Fe and Zn were unable to suppress viral progression. The mRNA transcriptional analysis for GFP and viral coat protein revealed that the transcripts of both genes were 5-fold reduced in response to C<sub>60</sub> treatment. Evaluation of the chloroplast ultrastructure showed that NMs treatment maintained the normal chloroplast structure in the plants as compared to untreated plants. C<sub>60</sub> upregulated the defense-related phytohormones (abscisic acid and salicylic acid) by 42–43%. Our results demonstrate the protective function of carbon-based NMs, with suppression of CCYV symptoms via inhibition of viral replication and systemic movement. |
| first_indexed | 2024-03-09T23:05:52Z |
| format | Article |
| id | doaj.art-660bacb69a2641a691e34b0c5beb5001 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T23:05:52Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-660bacb69a2641a691e34b0c5beb50012023-11-23T17:54:02ZengMDPI AGMicroorganisms2076-26072022-09-01109183710.3390/microorganisms10091837Manufactured Nano-Objects Confer Viral Protection against Cucurbit Chlorotic Yellows Virus (CCYV) Infecting <i>Nicotiana benthamiana</i>Mayasar I. Al-Zaban0Sadeq K. Alhag1Anas S. Dablool2Ahmed Ezzat Ahmed3Saad Alghamdi4Baber Ali5Fatimah A. Al-Saeed6Muhammad Hamzah Saleem7Peter Poczai8Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, Riyadh 11671, Saudi ArabiaBiology Department, College of Science and Arts, King Khalid University, Muhayil 61913, Saudi ArabiaDepartment of Public Health, Health Sciences College at Al-Leith, Umm Al-Qura University, Makkah 21955, Saudi ArabiaDepartment of Biology, College of Science, King Khalid University, Abha 61413, Saudi ArabiaLaboratory Medicine Department, Faculty of Applied Medical Sciences, Umm Al-Qura University, Makkah 24211, Saudi ArabiaDepartment of Plant Sciences, Quaid-i-Azam University, Islamabad 45320, PakistanDepartment of Biology, College of Science, King Khalid University, Abha 61413, Saudi ArabiaCollege of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaBotany Unit, Finnish Museum of Natural History, University of Helsinki, P.O. Box 7, FI-00014 Helsinki, FinlandNanotechnology has emerged as a new tool to combat phytopathogens in agricultural crops. Cucurbit chlorotic yellows virus (CCYV) mainly infects Solanaceae crops and causes significant crop losses. Nanomaterials (NMs) may have efficacy against plant viruses, but the mechanisms underlying complex nanomaterials-plant-virus interactions remain elusive. We challenged <i>Nicotiana benthamiana</i> plants with GFP-tagged CCYV and observed morphological, physiological, and molecular changes in response to 21-d foliar exposure to nanoscale Fe and Zn and C<sub>60</sub> fullerenes at 100 mg/L concentration for 21 days. We observed that in response to C<sub>60</sub> (100 mg/L) treatment, plants displayed a normal phenotype while the viral infection was not seen until 5 days post-inoculation. On the contrary, Fe and Zn were unable to suppress viral progression. The mRNA transcriptional analysis for GFP and viral coat protein revealed that the transcripts of both genes were 5-fold reduced in response to C<sub>60</sub> treatment. Evaluation of the chloroplast ultrastructure showed that NMs treatment maintained the normal chloroplast structure in the plants as compared to untreated plants. C<sub>60</sub> upregulated the defense-related phytohormones (abscisic acid and salicylic acid) by 42–43%. Our results demonstrate the protective function of carbon-based NMs, with suppression of CCYV symptoms via inhibition of viral replication and systemic movement.https://www.mdpi.com/2076-2607/10/9/1837C<sub>60</sub> based-NPscucurbit chlorotic yellows virusfoliar application |
| spellingShingle | Mayasar I. Al-Zaban Sadeq K. Alhag Anas S. Dablool Ahmed Ezzat Ahmed Saad Alghamdi Baber Ali Fatimah A. Al-Saeed Muhammad Hamzah Saleem Peter Poczai Manufactured Nano-Objects Confer Viral Protection against Cucurbit Chlorotic Yellows Virus (CCYV) Infecting <i>Nicotiana benthamiana</i> Microorganisms C<sub>60</sub> based-NPs cucurbit chlorotic yellows virus foliar application |
| title | Manufactured Nano-Objects Confer Viral Protection against Cucurbit Chlorotic Yellows Virus (CCYV) Infecting <i>Nicotiana benthamiana</i> |
| title_full | Manufactured Nano-Objects Confer Viral Protection against Cucurbit Chlorotic Yellows Virus (CCYV) Infecting <i>Nicotiana benthamiana</i> |
| title_fullStr | Manufactured Nano-Objects Confer Viral Protection against Cucurbit Chlorotic Yellows Virus (CCYV) Infecting <i>Nicotiana benthamiana</i> |
| title_full_unstemmed | Manufactured Nano-Objects Confer Viral Protection against Cucurbit Chlorotic Yellows Virus (CCYV) Infecting <i>Nicotiana benthamiana</i> |
| title_short | Manufactured Nano-Objects Confer Viral Protection against Cucurbit Chlorotic Yellows Virus (CCYV) Infecting <i>Nicotiana benthamiana</i> |
| title_sort | manufactured nano objects confer viral protection against cucurbit chlorotic yellows virus ccyv infecting i nicotiana benthamiana i |
| topic | C<sub>60</sub> based-NPs cucurbit chlorotic yellows virus foliar application |
| url | https://www.mdpi.com/2076-2607/10/9/1837 |
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