In vitro evaluation of the susceptibility of Acinetobacter baumannii isolates to antiseptics and disinfectants: comparison between clinical and environmental isolates
Abstract Background This study aims to assess the susceptibility of Acinetobacter baumannii isolates to the antiseptics and disinfectants commonly used, and to the non-approved product. Methods This is a prospective study carried out from February to August 2015, in the Bacteriology department of Mo...
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| Format: | Article |
| Language: | English |
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BMC
2017-04-01
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| Series: | Antimicrobial Resistance and Infection Control |
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| Online Access: | http://link.springer.com/article/10.1186/s13756-017-0195-y |
| _version_ | 1819015665258856448 |
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| author | Sanae Lanjri Jean Uwingabiye Mohammed Frikh Lina Abdellatifi Jalal Kasouati Adil Maleb Abdelouahed Bait Abdelhay Lemnouer Mostafa Elouennass |
| author_facet | Sanae Lanjri Jean Uwingabiye Mohammed Frikh Lina Abdellatifi Jalal Kasouati Adil Maleb Abdelouahed Bait Abdelhay Lemnouer Mostafa Elouennass |
| author_sort | Sanae Lanjri |
| collection | DOAJ |
| description | Abstract Background This study aims to assess the susceptibility of Acinetobacter baumannii isolates to the antiseptics and disinfectants commonly used, and to the non-approved product. Methods This is a prospective study carried out from February to August 2015, in the Bacteriology department of Mohammed V Military Teaching hospital of Rabat on A.baumannii isolates collected from colonized and/or infected patients and environmental samples. The antiseptics and disinfectants susceptibility testing was assessed using the micromethod validated in our department. The antiseptics and disinfectants studied were: 70% ethyl alcohol, chlorhexidine, povidone-iodine, didecyldimethylammonium chloride and a commercial product which was presented as a hospital disinfectant (non-registered product). Results Povidone-iodine, 0.5% chlorhexidine digluconate, 70% ethyl alcohol and didecyl dimethyl ammonium chloride in combination with N- (3-aminopropyl) -N-dodecylpropane-1, 3-diamine were effective against all the 81 A.baumannii isolates tested, and their logarithmic reduction ≥ 5 were observed in 100% of the isolates in their undiluted form. The strains isolated from patients were more resistant than environmental strains: at a dilution of ½ for 70% ethyl alcohol (37.77% vs 11.11%, p = 0.007) and at a dilution of 1/10 (100% vs 69.44%, p < 0.001) for povidone iodine. The non-registered product was ineffective with a resistance rate of 96.29% at a dilution of 1/50, 45.67% at a dilution of 1/10 and 13.58% in its purest form. Conclusion Our study revealed the effectiveness of the main disinfectants and antiseptics used in Morocco; three antiseptics tested were effective in their purest form against the 81 A.baumannii isolates. Regarding disinfectants, our results showed an efficacy of didecyl dimethyl ammonium at the recommended use concentration and in its purest form. This study emphasizes the need for using disinfectants and antiseptics in dilutions recommended by the manufacturer because the insufficient dilutions of these products are not effective. Our findings also demonstrated an inefficiency of the non-registered product against A.baumanii isolates. However, the non-registered products should be prohibited. |
| first_indexed | 2024-12-21T02:35:21Z |
| format | Article |
| id | doaj.art-660c23f593544afbb2ab516cc5bd90a1 |
| institution | Directory Open Access Journal |
| issn | 2047-2994 |
| language | English |
| last_indexed | 2024-12-21T02:35:21Z |
| publishDate | 2017-04-01 |
| publisher | BMC |
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| series | Antimicrobial Resistance and Infection Control |
| spelling | doaj.art-660c23f593544afbb2ab516cc5bd90a12022-12-21T19:18:50ZengBMCAntimicrobial Resistance and Infection Control2047-29942017-04-01611710.1186/s13756-017-0195-yIn vitro evaluation of the susceptibility of Acinetobacter baumannii isolates to antiseptics and disinfectants: comparison between clinical and environmental isolatesSanae Lanjri0Jean Uwingabiye1Mohammed Frikh2Lina Abdellatifi3Jalal Kasouati4Adil Maleb5Abdelouahed Bait6Abdelhay Lemnouer7Mostafa Elouennass8Department of Clinical Bacteriology, Mohammed V Military teaching hospital, research team of Epidemiology and Bacterial resistance, Faculty of Medicine and Pharmacy, Mohammed V UniversityDepartment of Clinical Bacteriology, Mohammed V Military teaching hospital, research team of Epidemiology and Bacterial resistance, Faculty of Medicine and Pharmacy, Mohammed V UniversityDepartment of Clinical Bacteriology, Mohammed V Military teaching hospital, research team of Epidemiology and Bacterial resistance, Faculty of Medicine and Pharmacy, Mohammed V UniversityDepartment of Clinical Bacteriology, Mohammed V Military teaching hospital, research team of Epidemiology and Bacterial resistance, Faculty of Medicine and Pharmacy, Mohammed V UniversityDepartment of Clinical Bacteriology, Mohammed V Military teaching hospital, research team of Epidemiology and Bacterial resistance, Faculty of Medicine and Pharmacy, Mohammed V UniversityDepartment of Clinical Bacteriology, Mohammed V Military teaching hospital, research team of Epidemiology and Bacterial resistance, Faculty of Medicine and Pharmacy, Mohammed V UniversityDepartment of Clinical Bacteriology, Mohammed V Military teaching hospital, research team of Epidemiology and Bacterial resistance, Faculty of Medicine and Pharmacy, Mohammed V UniversityDepartment of Clinical Bacteriology, Mohammed V Military teaching hospital, research team of Epidemiology and Bacterial resistance, Faculty of Medicine and Pharmacy, Mohammed V UniversityDepartment of Clinical Bacteriology, Mohammed V Military teaching hospital, research team of Epidemiology and Bacterial resistance, Faculty of Medicine and Pharmacy, Mohammed V UniversityAbstract Background This study aims to assess the susceptibility of Acinetobacter baumannii isolates to the antiseptics and disinfectants commonly used, and to the non-approved product. Methods This is a prospective study carried out from February to August 2015, in the Bacteriology department of Mohammed V Military Teaching hospital of Rabat on A.baumannii isolates collected from colonized and/or infected patients and environmental samples. The antiseptics and disinfectants susceptibility testing was assessed using the micromethod validated in our department. The antiseptics and disinfectants studied were: 70% ethyl alcohol, chlorhexidine, povidone-iodine, didecyldimethylammonium chloride and a commercial product which was presented as a hospital disinfectant (non-registered product). Results Povidone-iodine, 0.5% chlorhexidine digluconate, 70% ethyl alcohol and didecyl dimethyl ammonium chloride in combination with N- (3-aminopropyl) -N-dodecylpropane-1, 3-diamine were effective against all the 81 A.baumannii isolates tested, and their logarithmic reduction ≥ 5 were observed in 100% of the isolates in their undiluted form. The strains isolated from patients were more resistant than environmental strains: at a dilution of ½ for 70% ethyl alcohol (37.77% vs 11.11%, p = 0.007) and at a dilution of 1/10 (100% vs 69.44%, p < 0.001) for povidone iodine. The non-registered product was ineffective with a resistance rate of 96.29% at a dilution of 1/50, 45.67% at a dilution of 1/10 and 13.58% in its purest form. Conclusion Our study revealed the effectiveness of the main disinfectants and antiseptics used in Morocco; three antiseptics tested were effective in their purest form against the 81 A.baumannii isolates. Regarding disinfectants, our results showed an efficacy of didecyl dimethyl ammonium at the recommended use concentration and in its purest form. This study emphasizes the need for using disinfectants and antiseptics in dilutions recommended by the manufacturer because the insufficient dilutions of these products are not effective. Our findings also demonstrated an inefficiency of the non-registered product against A.baumanii isolates. However, the non-registered products should be prohibited.http://link.springer.com/article/10.1186/s13756-017-0195-yAcinetobacter baumanniiResistanceAntisepticsDisinfectants |
| spellingShingle | Sanae Lanjri Jean Uwingabiye Mohammed Frikh Lina Abdellatifi Jalal Kasouati Adil Maleb Abdelouahed Bait Abdelhay Lemnouer Mostafa Elouennass In vitro evaluation of the susceptibility of Acinetobacter baumannii isolates to antiseptics and disinfectants: comparison between clinical and environmental isolates Antimicrobial Resistance and Infection Control Acinetobacter baumannii Resistance Antiseptics Disinfectants |
| title | In vitro evaluation of the susceptibility of Acinetobacter baumannii isolates to antiseptics and disinfectants: comparison between clinical and environmental isolates |
| title_full | In vitro evaluation of the susceptibility of Acinetobacter baumannii isolates to antiseptics and disinfectants: comparison between clinical and environmental isolates |
| title_fullStr | In vitro evaluation of the susceptibility of Acinetobacter baumannii isolates to antiseptics and disinfectants: comparison between clinical and environmental isolates |
| title_full_unstemmed | In vitro evaluation of the susceptibility of Acinetobacter baumannii isolates to antiseptics and disinfectants: comparison between clinical and environmental isolates |
| title_short | In vitro evaluation of the susceptibility of Acinetobacter baumannii isolates to antiseptics and disinfectants: comparison between clinical and environmental isolates |
| title_sort | in vitro evaluation of the susceptibility of acinetobacter baumannii isolates to antiseptics and disinfectants comparison between clinical and environmental isolates |
| topic | Acinetobacter baumannii Resistance Antiseptics Disinfectants |
| url | http://link.springer.com/article/10.1186/s13756-017-0195-y |
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