MILT DILUTION EFFECTIVENESS ON PIKEPERCH (SANDER LUCIOPERCA) SPERM DNA INACTIVATION
Percid fishes, including pikeperch (Sander lucioperca) have recently become the subject of intense research. In order to obtain gynogenetic all female pikeperch populations, normal pikeperch eggs are fertilized with inactivated sperm. Because pikeperch semen has a high viscosity, milt has to be dilu...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Agroprint Timisoara
2023-10-01
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| Series: | Scientific Papers Animal Science and Biotechnologies |
| Subjects: | |
| Online Access: | https://spasb.ro/index.php/public_html/article/view/1441 |
| Summary: | Percid fishes, including pikeperch (Sander lucioperca) have recently become the
subject of intense research. In order to obtain gynogenetic all female pikeperch
populations, normal pikeperch eggs are fertilized with inactivated sperm. Because
pikeperch semen has a high viscosity, milt has to be diluted in an immobilizing
solution before DNA inactivation. The aim of this study was to assess milt diluting
solutions effectiveness in order to inactivate sperm DNA with UV irradiation, to
produce meiotic gynogenetic pikeperch (Sander lucioperca). We assessed sperm
motility after dilution in 5 different immobilizing solutions. Best results were
obtained using Ringer’s solution as pikeperch sperm diluent. |
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| ISSN: | 1841-9364 2344-4576 |