Phenolic Profile, EPR Determination, and Antiproliferative Activity against Human Cancer Cell Lines of <i>Anthyllis vulneraria</i> Extracts

In the current work, the leaf and flower extracts of <i>Anthyllis vulneraria</i> were evaluated for their chemical characterization using HPLC-MS and for their radical scavenging capacity toward methoxy radicals produced by a Fenton-type reaction using an electron paramagnetic resonance...

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Main Authors: Manel Ouerfelli, Isidoro Metón, Idoia Codina-Torrella, María Pilar Almajano
Format: Article
Language:English
Published: MDPI AG 2022-11-01
Series:Molecules
Subjects:
Online Access:https://www.mdpi.com/1420-3049/27/21/7495
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author Manel Ouerfelli
Isidoro Metón
Idoia Codina-Torrella
María Pilar Almajano
author_facet Manel Ouerfelli
Isidoro Metón
Idoia Codina-Torrella
María Pilar Almajano
author_sort Manel Ouerfelli
collection DOAJ
description In the current work, the leaf and flower extracts of <i>Anthyllis vulneraria</i> were evaluated for their chemical characterization using HPLC-MS and for their radical scavenging capacity toward methoxy radicals produced by a Fenton-type reaction using an electron paramagnetic resonance (EPR) spectroscopy assay. The in vitro antiproliferative activity of these extracts against several human-derived cancer cells (breast: MCF-7; cervical: HeLa; hepatocellular: HepG2) was also evaluated. The results showed that the <i>Anthyllis vulneraria</i> leaf extract was characterized by 17 different phenolic compounds, among which phenolic acids were the most abundant, while its flower extract exhibited higher contents of flavonoids. Furthermore, <i>Anthyllis vulneraria</i> extracts demonstrated a potent radical scavenging activity against methoxy radicals. Both extracts also significantly reduced the viability of the different cancer cell lines. The results of the current study suggested that <i>Anthyllis vulneraria</i> extracts are a promising source of antioxidant compounds with health benefits and pointed to their potential use for treating cancer and developing novel therapeutic agents.
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spelling doaj.art-660d2f82921c459db09ae273de954d742023-11-24T06:05:10ZengMDPI AGMolecules1420-30492022-11-012721749510.3390/molecules27217495Phenolic Profile, EPR Determination, and Antiproliferative Activity against Human Cancer Cell Lines of <i>Anthyllis vulneraria</i> ExtractsManel Ouerfelli0Isidoro Metón1Idoia Codina-Torrella2María Pilar Almajano3Chemical Engineering Department, Escola Tècnica Superior d’Enginyeria Industrial de Barcelona (ETSEIB), Universitat Politècnica de Catalunya, Av. Diagonal 647, 08028 Barcelona, SpainBiochemistry and Physiology Departament, Facultat de Farmàcia i Ciències de l’Alimentació, Universitat de Barcelona, Joan XXIII 27–31, 08028 Barcelona, SpainAgri-Food Engineering and Biotechnology Department, Escola d’Enginyeria Agroalimentària i de Biosistemes de Bacelona (EEABB), Universitat Politècnica de Catalunya, Esteve Terrades 8, 08860 Castelldefels, SpainChemical Engineering Department, Escola Tècnica Superior d’Enginyeria Industrial de Barcelona (ETSEIB), Universitat Politècnica de Catalunya, Av. Diagonal 647, 08028 Barcelona, SpainIn the current work, the leaf and flower extracts of <i>Anthyllis vulneraria</i> were evaluated for their chemical characterization using HPLC-MS and for their radical scavenging capacity toward methoxy radicals produced by a Fenton-type reaction using an electron paramagnetic resonance (EPR) spectroscopy assay. The in vitro antiproliferative activity of these extracts against several human-derived cancer cells (breast: MCF-7; cervical: HeLa; hepatocellular: HepG2) was also evaluated. The results showed that the <i>Anthyllis vulneraria</i> leaf extract was characterized by 17 different phenolic compounds, among which phenolic acids were the most abundant, while its flower extract exhibited higher contents of flavonoids. Furthermore, <i>Anthyllis vulneraria</i> extracts demonstrated a potent radical scavenging activity against methoxy radicals. Both extracts also significantly reduced the viability of the different cancer cell lines. The results of the current study suggested that <i>Anthyllis vulneraria</i> extracts are a promising source of antioxidant compounds with health benefits and pointed to their potential use for treating cancer and developing novel therapeutic agents.https://www.mdpi.com/1420-3049/27/21/7495<i>Anthyllis vulneraria</i>HPLC-MSEPRmethoxy radicalsantiproliferative activityhuman-derived cancer cells
spellingShingle Manel Ouerfelli
Isidoro Metón
Idoia Codina-Torrella
María Pilar Almajano
Phenolic Profile, EPR Determination, and Antiproliferative Activity against Human Cancer Cell Lines of <i>Anthyllis vulneraria</i> Extracts
Molecules
<i>Anthyllis vulneraria</i>
HPLC-MS
EPR
methoxy radicals
antiproliferative activity
human-derived cancer cells
title Phenolic Profile, EPR Determination, and Antiproliferative Activity against Human Cancer Cell Lines of <i>Anthyllis vulneraria</i> Extracts
title_full Phenolic Profile, EPR Determination, and Antiproliferative Activity against Human Cancer Cell Lines of <i>Anthyllis vulneraria</i> Extracts
title_fullStr Phenolic Profile, EPR Determination, and Antiproliferative Activity against Human Cancer Cell Lines of <i>Anthyllis vulneraria</i> Extracts
title_full_unstemmed Phenolic Profile, EPR Determination, and Antiproliferative Activity against Human Cancer Cell Lines of <i>Anthyllis vulneraria</i> Extracts
title_short Phenolic Profile, EPR Determination, and Antiproliferative Activity against Human Cancer Cell Lines of <i>Anthyllis vulneraria</i> Extracts
title_sort phenolic profile epr determination and antiproliferative activity against human cancer cell lines of i anthyllis vulneraria i extracts
topic <i>Anthyllis vulneraria</i>
HPLC-MS
EPR
methoxy radicals
antiproliferative activity
human-derived cancer cells
url https://www.mdpi.com/1420-3049/27/21/7495
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