Phenolic Profile, EPR Determination, and Antiproliferative Activity against Human Cancer Cell Lines of <i>Anthyllis vulneraria</i> Extracts
In the current work, the leaf and flower extracts of <i>Anthyllis vulneraria</i> were evaluated for their chemical characterization using HPLC-MS and for their radical scavenging capacity toward methoxy radicals produced by a Fenton-type reaction using an electron paramagnetic resonance...
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MDPI AG
2022-11-01
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author | Manel Ouerfelli Isidoro Metón Idoia Codina-Torrella María Pilar Almajano |
author_facet | Manel Ouerfelli Isidoro Metón Idoia Codina-Torrella María Pilar Almajano |
author_sort | Manel Ouerfelli |
collection | DOAJ |
description | In the current work, the leaf and flower extracts of <i>Anthyllis vulneraria</i> were evaluated for their chemical characterization using HPLC-MS and for their radical scavenging capacity toward methoxy radicals produced by a Fenton-type reaction using an electron paramagnetic resonance (EPR) spectroscopy assay. The in vitro antiproliferative activity of these extracts against several human-derived cancer cells (breast: MCF-7; cervical: HeLa; hepatocellular: HepG2) was also evaluated. The results showed that the <i>Anthyllis vulneraria</i> leaf extract was characterized by 17 different phenolic compounds, among which phenolic acids were the most abundant, while its flower extract exhibited higher contents of flavonoids. Furthermore, <i>Anthyllis vulneraria</i> extracts demonstrated a potent radical scavenging activity against methoxy radicals. Both extracts also significantly reduced the viability of the different cancer cell lines. The results of the current study suggested that <i>Anthyllis vulneraria</i> extracts are a promising source of antioxidant compounds with health benefits and pointed to their potential use for treating cancer and developing novel therapeutic agents. |
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language | English |
last_indexed | 2024-03-09T18:47:56Z |
publishDate | 2022-11-01 |
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spelling | doaj.art-660d2f82921c459db09ae273de954d742023-11-24T06:05:10ZengMDPI AGMolecules1420-30492022-11-012721749510.3390/molecules27217495Phenolic Profile, EPR Determination, and Antiproliferative Activity against Human Cancer Cell Lines of <i>Anthyllis vulneraria</i> ExtractsManel Ouerfelli0Isidoro Metón1Idoia Codina-Torrella2María Pilar Almajano3Chemical Engineering Department, Escola Tècnica Superior d’Enginyeria Industrial de Barcelona (ETSEIB), Universitat Politècnica de Catalunya, Av. Diagonal 647, 08028 Barcelona, SpainBiochemistry and Physiology Departament, Facultat de Farmàcia i Ciències de l’Alimentació, Universitat de Barcelona, Joan XXIII 27–31, 08028 Barcelona, SpainAgri-Food Engineering and Biotechnology Department, Escola d’Enginyeria Agroalimentària i de Biosistemes de Bacelona (EEABB), Universitat Politècnica de Catalunya, Esteve Terrades 8, 08860 Castelldefels, SpainChemical Engineering Department, Escola Tècnica Superior d’Enginyeria Industrial de Barcelona (ETSEIB), Universitat Politècnica de Catalunya, Av. Diagonal 647, 08028 Barcelona, SpainIn the current work, the leaf and flower extracts of <i>Anthyllis vulneraria</i> were evaluated for their chemical characterization using HPLC-MS and for their radical scavenging capacity toward methoxy radicals produced by a Fenton-type reaction using an electron paramagnetic resonance (EPR) spectroscopy assay. The in vitro antiproliferative activity of these extracts against several human-derived cancer cells (breast: MCF-7; cervical: HeLa; hepatocellular: HepG2) was also evaluated. The results showed that the <i>Anthyllis vulneraria</i> leaf extract was characterized by 17 different phenolic compounds, among which phenolic acids were the most abundant, while its flower extract exhibited higher contents of flavonoids. Furthermore, <i>Anthyllis vulneraria</i> extracts demonstrated a potent radical scavenging activity against methoxy radicals. Both extracts also significantly reduced the viability of the different cancer cell lines. The results of the current study suggested that <i>Anthyllis vulneraria</i> extracts are a promising source of antioxidant compounds with health benefits and pointed to their potential use for treating cancer and developing novel therapeutic agents.https://www.mdpi.com/1420-3049/27/21/7495<i>Anthyllis vulneraria</i>HPLC-MSEPRmethoxy radicalsantiproliferative activityhuman-derived cancer cells |
spellingShingle | Manel Ouerfelli Isidoro Metón Idoia Codina-Torrella María Pilar Almajano Phenolic Profile, EPR Determination, and Antiproliferative Activity against Human Cancer Cell Lines of <i>Anthyllis vulneraria</i> Extracts Molecules <i>Anthyllis vulneraria</i> HPLC-MS EPR methoxy radicals antiproliferative activity human-derived cancer cells |
title | Phenolic Profile, EPR Determination, and Antiproliferative Activity against Human Cancer Cell Lines of <i>Anthyllis vulneraria</i> Extracts |
title_full | Phenolic Profile, EPR Determination, and Antiproliferative Activity against Human Cancer Cell Lines of <i>Anthyllis vulneraria</i> Extracts |
title_fullStr | Phenolic Profile, EPR Determination, and Antiproliferative Activity against Human Cancer Cell Lines of <i>Anthyllis vulneraria</i> Extracts |
title_full_unstemmed | Phenolic Profile, EPR Determination, and Antiproliferative Activity against Human Cancer Cell Lines of <i>Anthyllis vulneraria</i> Extracts |
title_short | Phenolic Profile, EPR Determination, and Antiproliferative Activity against Human Cancer Cell Lines of <i>Anthyllis vulneraria</i> Extracts |
title_sort | phenolic profile epr determination and antiproliferative activity against human cancer cell lines of i anthyllis vulneraria i extracts |
topic | <i>Anthyllis vulneraria</i> HPLC-MS EPR methoxy radicals antiproliferative activity human-derived cancer cells |
url | https://www.mdpi.com/1420-3049/27/21/7495 |
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