NS1-based ELISA test efficiently detects dengue infections without cross-reactivity with Zika virus
Objectives: The aim of this study was to achieve greater specificity of dengue virus (DENV) serological tests based on a recombinant antigen derived from non-structural protein 1 (ΔNS1) with regard to cross-reactive Zika virus (ZIKV) anti-NS1 antibody responses. This is of relevance in endemic regio...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-11-01
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| Series: | International Journal of Infectious Diseases |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1201971221007190 |
| _version_ | 1818891606313402368 |
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| author | Samuel Santos Pereira Robert Andreata-Santos Lennon Ramos Pereira Camila Pereira Soares Alvina Clara Félix Patrícia de Mello Jungmann Cardoso de Andrade Edison Luís Durigon Camila Malta Romano Luís Carlos de Souza Ferreira |
| author_facet | Samuel Santos Pereira Robert Andreata-Santos Lennon Ramos Pereira Camila Pereira Soares Alvina Clara Félix Patrícia de Mello Jungmann Cardoso de Andrade Edison Luís Durigon Camila Malta Romano Luís Carlos de Souza Ferreira |
| author_sort | Samuel Santos Pereira |
| collection | DOAJ |
| description | Objectives: The aim of this study was to achieve greater specificity of dengue virus (DENV) serological tests based on a recombinant antigen derived from non-structural protein 1 (ΔNS1) with regard to cross-reactive Zika virus (ZIKV) anti-NS1 antibody responses. This is of relevance in endemic regions for the serological discrimination of both DENV and ZIKV, such as Brazil and other tropical countries. Methods: The ΔNS1 proteins were obtained as recombinant antigens and were evaluated as solid-phase-bound antigens in the ELISA test to detect anti-NS1 IgG antibodies. The performance of the ∆NS1-based DENV IgG ELISA was assessed with both mouse and human serum samples previously exposed to DENV or ZIKV. Results: The ∆NS1-based DENV IgG ELISA detected anti-DENV NS1 IgG without cross-reactivity with ZIKV-positive serum samples. The sensitivity and specificity of the assay determined using samples previously characterized by real-time PCR (qRT-PCR) or plaque reduction neutralization assay (PRNT) were 82% and 93%, respectively. Conclusion: The ∆NS1-based DENV IgG ELISA conferred enhanced diagnostic specificity for anti-DENV serological tests and may be particularly useful for serological analyses in endemic regions for both DENV and ZIKV transmission. |
| first_indexed | 2024-12-19T17:43:29Z |
| format | Article |
| id | doaj.art-660dc5acfb314465b99606cb9fe8d15a |
| institution | Directory Open Access Journal |
| issn | 1201-9712 |
| language | English |
| last_indexed | 2024-12-19T17:43:29Z |
| publishDate | 2021-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal of Infectious Diseases |
| spelling | doaj.art-660dc5acfb314465b99606cb9fe8d15a2022-12-21T20:12:08ZengElsevierInternational Journal of Infectious Diseases1201-97122021-11-01112202204NS1-based ELISA test efficiently detects dengue infections without cross-reactivity with Zika virusSamuel Santos Pereira0Robert Andreata-Santos1Lennon Ramos Pereira2Camila Pereira Soares3Alvina Clara Félix4Patrícia de Mello Jungmann Cardoso de Andrade5Edison Luís Durigon6Camila Malta Romano7Luís Carlos de Souza Ferreira8Vaccine Development Laboratory, Microbiology Department, Institute of Biomedical Sciences, University of São Paulo, São Paulo, BrazilVaccine Development Laboratory, Microbiology Department, Institute of Biomedical Sciences, University of São Paulo, São Paulo, BrazilVaccine Development Laboratory, Microbiology Department, Institute of Biomedical Sciences, University of São Paulo, São Paulo, BrazilLaboratory of Clinical and Molecular Virology, Microbiology Department, Institute of Biomedical Sciences, University of São Paulo, São Paulo, BrazilInstituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo, BrazilUniversidade de Pernambuco UPE – General Pathology, Recife, Pernambuco, BrazilLaboratory of Clinical and Molecular Virology, Microbiology Department, Institute of Biomedical Sciences, University of São Paulo, São Paulo, BrazilInstituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo, Brazil; Hospital das Clínicas HCFMUSP (LIM 52), Faculdade de Medicina, Universidade de São Paulo, São Paulo, BrazilVaccine Development Laboratory, Microbiology Department, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil; Corresponding author: Luís Carlos de Souza Ferreira, Vaccine Development Laboratory, Microbiology Department, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.Objectives: The aim of this study was to achieve greater specificity of dengue virus (DENV) serological tests based on a recombinant antigen derived from non-structural protein 1 (ΔNS1) with regard to cross-reactive Zika virus (ZIKV) anti-NS1 antibody responses. This is of relevance in endemic regions for the serological discrimination of both DENV and ZIKV, such as Brazil and other tropical countries. Methods: The ΔNS1 proteins were obtained as recombinant antigens and were evaluated as solid-phase-bound antigens in the ELISA test to detect anti-NS1 IgG antibodies. The performance of the ∆NS1-based DENV IgG ELISA was assessed with both mouse and human serum samples previously exposed to DENV or ZIKV. Results: The ∆NS1-based DENV IgG ELISA detected anti-DENV NS1 IgG without cross-reactivity with ZIKV-positive serum samples. The sensitivity and specificity of the assay determined using samples previously characterized by real-time PCR (qRT-PCR) or plaque reduction neutralization assay (PRNT) were 82% and 93%, respectively. Conclusion: The ∆NS1-based DENV IgG ELISA conferred enhanced diagnostic specificity for anti-DENV serological tests and may be particularly useful for serological analyses in endemic regions for both DENV and ZIKV transmission.http://www.sciencedirect.com/science/article/pii/S1201971221007190Dengue virusSerological diagnosisNS1ELISA |
| spellingShingle | Samuel Santos Pereira Robert Andreata-Santos Lennon Ramos Pereira Camila Pereira Soares Alvina Clara Félix Patrícia de Mello Jungmann Cardoso de Andrade Edison Luís Durigon Camila Malta Romano Luís Carlos de Souza Ferreira NS1-based ELISA test efficiently detects dengue infections without cross-reactivity with Zika virus International Journal of Infectious Diseases Dengue virus Serological diagnosis NS1 ELISA |
| title | NS1-based ELISA test efficiently detects dengue infections without cross-reactivity with Zika virus |
| title_full | NS1-based ELISA test efficiently detects dengue infections without cross-reactivity with Zika virus |
| title_fullStr | NS1-based ELISA test efficiently detects dengue infections without cross-reactivity with Zika virus |
| title_full_unstemmed | NS1-based ELISA test efficiently detects dengue infections without cross-reactivity with Zika virus |
| title_short | NS1-based ELISA test efficiently detects dengue infections without cross-reactivity with Zika virus |
| title_sort | ns1 based elisa test efficiently detects dengue infections without cross reactivity with zika virus |
| topic | Dengue virus Serological diagnosis NS1 ELISA |
| url | http://www.sciencedirect.com/science/article/pii/S1201971221007190 |
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