Noise Filtering Method of Digital Holographic Microscopy for Obtaining an Accurate Three-Dimensional Profile of Object Using a Windowed Sideband Array (WiSA)

In the image processing method of digital holographic microscopy (DHM), we can obtain a phase information of an object by windowing a sideband in Fourier domain and taking inverse Fourier transform. In this method, it is necessary to window a wide sideband to obtain detailed information on the objec...

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Main Authors: Hyun-Woo Kim, Myungjin Cho, Min-Chul Lee
Format: Article
Language:English
Published: MDPI AG 2022-06-01
Series:Sensors
Subjects:
Online Access:https://www.mdpi.com/1424-8220/22/13/4844
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author Hyun-Woo Kim
Myungjin Cho
Min-Chul Lee
author_facet Hyun-Woo Kim
Myungjin Cho
Min-Chul Lee
author_sort Hyun-Woo Kim
collection DOAJ
description In the image processing method of digital holographic microscopy (DHM), we can obtain a phase information of an object by windowing a sideband in Fourier domain and taking inverse Fourier transform. In this method, it is necessary to window a wide sideband to obtain detailed information on the object. However, since the information of the DC spectrum is widely distributed over the entire range from the center of Fourier domain, the window sideband includes not only phase information but also DC information. For this reason, research on acquiring only the phase information of an object without noise in digital holography is a challenging issue for many researchers. Therefore, in this paper, we propose the use of a windowed sideband array (WiSA) as an image processing method to obtain an accurate three-dimensional (3D) profile of an object without noise in DHM. The proposed method does not affect the neighbor pixels of the filtered pixel but removes noise while maintaining the detail of the object. Thus, a more accurate 3D profile can be obtained compared with the conventional filter. In this paper, we create an ideal comparison target i.e., microspheres for comparison, and verify the effect of the filter through additional experiments using red blood cells.
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spelling doaj.art-6614920bc41b40ccbcb4ddc8b676d7d22023-12-03T14:22:18ZengMDPI AGSensors1424-82202022-06-012213484410.3390/s22134844Noise Filtering Method of Digital Holographic Microscopy for Obtaining an Accurate Three-Dimensional Profile of Object Using a Windowed Sideband Array (WiSA)Hyun-Woo Kim0Myungjin Cho1Min-Chul Lee2Department of Computer Science and Networks, Kyushu Institute of Technology, 680-4 Kawazu, Fukuoka 820-8502, JapanSchool of ICT, Robotics, and Mechanical Engineering, Research Center for Hyper-Connected Convergence Technology, IITC, Hankyong National University, 327 Chungang-ro, Anseong 17579, KoreaDepartment of Computer Science and Networks, Kyushu Institute of Technology, 680-4 Kawazu, Fukuoka 820-8502, JapanIn the image processing method of digital holographic microscopy (DHM), we can obtain a phase information of an object by windowing a sideband in Fourier domain and taking inverse Fourier transform. In this method, it is necessary to window a wide sideband to obtain detailed information on the object. However, since the information of the DC spectrum is widely distributed over the entire range from the center of Fourier domain, the window sideband includes not only phase information but also DC information. For this reason, research on acquiring only the phase information of an object without noise in digital holography is a challenging issue for many researchers. Therefore, in this paper, we propose the use of a windowed sideband array (WiSA) as an image processing method to obtain an accurate three-dimensional (3D) profile of an object without noise in DHM. The proposed method does not affect the neighbor pixels of the filtered pixel but removes noise while maintaining the detail of the object. Thus, a more accurate 3D profile can be obtained compared with the conventional filter. In this paper, we create an ideal comparison target i.e., microspheres for comparison, and verify the effect of the filter through additional experiments using red blood cells.https://www.mdpi.com/1424-8220/22/13/4844three-dimensional imagingnoise reductiondigital holographic microscopy
spellingShingle Hyun-Woo Kim
Myungjin Cho
Min-Chul Lee
Noise Filtering Method of Digital Holographic Microscopy for Obtaining an Accurate Three-Dimensional Profile of Object Using a Windowed Sideband Array (WiSA)
Sensors
three-dimensional imaging
noise reduction
digital holographic microscopy
title Noise Filtering Method of Digital Holographic Microscopy for Obtaining an Accurate Three-Dimensional Profile of Object Using a Windowed Sideband Array (WiSA)
title_full Noise Filtering Method of Digital Holographic Microscopy for Obtaining an Accurate Three-Dimensional Profile of Object Using a Windowed Sideband Array (WiSA)
title_fullStr Noise Filtering Method of Digital Holographic Microscopy for Obtaining an Accurate Three-Dimensional Profile of Object Using a Windowed Sideband Array (WiSA)
title_full_unstemmed Noise Filtering Method of Digital Holographic Microscopy for Obtaining an Accurate Three-Dimensional Profile of Object Using a Windowed Sideband Array (WiSA)
title_short Noise Filtering Method of Digital Holographic Microscopy for Obtaining an Accurate Three-Dimensional Profile of Object Using a Windowed Sideband Array (WiSA)
title_sort noise filtering method of digital holographic microscopy for obtaining an accurate three dimensional profile of object using a windowed sideband array wisa
topic three-dimensional imaging
noise reduction
digital holographic microscopy
url https://www.mdpi.com/1424-8220/22/13/4844
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