Cranberry Proanthocyanidins-PANI Nanocomposite for the Detection of Bacteria Associated with Urinary Tract Infections
Consumption of cranberries is associated with the putative effects of preventing urinary tract infections (UTIs). Cranberry proanthocyanidins (PAC) contain unusual double A-type linkages, which are associated with strong interactions with surface virulence factors found on UTI-causing bacteria such...
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| Format: | Article |
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MDPI AG
2021-06-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/11/6/199 |
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| author | Hilary Urena-Saborio Anu Prathap M. Udayan Emilia Alfaro-Viquez Sergio Madrigal-Carballo Jess D. Reed Sundaram Gunasekaran |
| author_facet | Hilary Urena-Saborio Anu Prathap M. Udayan Emilia Alfaro-Viquez Sergio Madrigal-Carballo Jess D. Reed Sundaram Gunasekaran |
| author_sort | Hilary Urena-Saborio |
| collection | DOAJ |
| description | Consumption of cranberries is associated with the putative effects of preventing urinary tract infections (UTIs). Cranberry proanthocyanidins (PAC) contain unusual double A-type linkages, which are associated with strong interactions with surface virulence factors found on UTI-causing bacteria such as extra-intestinal pathogenic <i>Escherichia coli</i> (ExPEC), depicting in bacterial agglutination processes. In this work, we demonstrated the efficacy of cranberry PAC (200 μg/mL) to agglutinate ExPEC (5.0 × 10<sup>8</sup> CFU/mL) in vitro as a selective interaction for the design of functionalized biosensors for potential detection of UTIs. We fabricated functionalized screen-printed electrodes (SPEs) by modifying with PAC-polyaniline (PANI) nanocomposites and tested the effectiveness of the PAC-PANI/SPE biosensor for detecting the presence of ExPEC in aqueous suspensions. Results indicated that the PAC-PANI/SPE was highly sensitive (limit of quantification of 1 CFU/mL of ExPEC), and its response was linear over the concentration range of 1–70,000 CFU/mL, suggesting cranberry PAC-functionalized biosensors are an innovative alternative for the detection and diagnosis of ExPEC-associated UTIs. The biosensor was also highly selective, reproducible, and stable. |
| first_indexed | 2024-03-10T10:15:36Z |
| format | Article |
| id | doaj.art-661ae3b664c54ea68adcdf84a251366c |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T10:15:36Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-661ae3b664c54ea68adcdf84a251366c2023-11-22T00:49:36ZengMDPI AGBiosensors2079-63742021-06-0111619910.3390/bios11060199Cranberry Proanthocyanidins-PANI Nanocomposite for the Detection of Bacteria Associated with Urinary Tract InfectionsHilary Urena-Saborio0Anu Prathap M. Udayan1Emilia Alfaro-Viquez2Sergio Madrigal-Carballo3Jess D. Reed4Sundaram Gunasekaran5Biosensors and Nanotechnology Laboratory, Department of Biological Systems Engineering, University of Wisconsin-Madison, 460 Henry Mall, Madison, WI 53706, USABiosensors and Nanotechnology Laboratory, Department of Biological Systems Engineering, University of Wisconsin-Madison, 460 Henry Mall, Madison, WI 53706, USAReed Research Group, Department of Animal Sciences, University of Wisconsin-Madison, 1675 Observatory Dr, Madison, WI 53706, USAReed Research Group, Department of Animal Sciences, University of Wisconsin-Madison, 1675 Observatory Dr, Madison, WI 53706, USAReed Research Group, Department of Animal Sciences, University of Wisconsin-Madison, 1675 Observatory Dr, Madison, WI 53706, USABiosensors and Nanotechnology Laboratory, Department of Biological Systems Engineering, University of Wisconsin-Madison, 460 Henry Mall, Madison, WI 53706, USAConsumption of cranberries is associated with the putative effects of preventing urinary tract infections (UTIs). Cranberry proanthocyanidins (PAC) contain unusual double A-type linkages, which are associated with strong interactions with surface virulence factors found on UTI-causing bacteria such as extra-intestinal pathogenic <i>Escherichia coli</i> (ExPEC), depicting in bacterial agglutination processes. In this work, we demonstrated the efficacy of cranberry PAC (200 μg/mL) to agglutinate ExPEC (5.0 × 10<sup>8</sup> CFU/mL) in vitro as a selective interaction for the design of functionalized biosensors for potential detection of UTIs. We fabricated functionalized screen-printed electrodes (SPEs) by modifying with PAC-polyaniline (PANI) nanocomposites and tested the effectiveness of the PAC-PANI/SPE biosensor for detecting the presence of ExPEC in aqueous suspensions. Results indicated that the PAC-PANI/SPE was highly sensitive (limit of quantification of 1 CFU/mL of ExPEC), and its response was linear over the concentration range of 1–70,000 CFU/mL, suggesting cranberry PAC-functionalized biosensors are an innovative alternative for the detection and diagnosis of ExPEC-associated UTIs. The biosensor was also highly selective, reproducible, and stable.https://www.mdpi.com/2079-6374/11/6/199proanthocyanidinsnanocompositesurinary tract infectionsextra-intestinal pathogenic <i>Escherichia coli</i>bacteria agglutination |
| spellingShingle | Hilary Urena-Saborio Anu Prathap M. Udayan Emilia Alfaro-Viquez Sergio Madrigal-Carballo Jess D. Reed Sundaram Gunasekaran Cranberry Proanthocyanidins-PANI Nanocomposite for the Detection of Bacteria Associated with Urinary Tract Infections Biosensors proanthocyanidins nanocomposites urinary tract infections extra-intestinal pathogenic <i>Escherichia coli</i> bacteria agglutination |
| title | Cranberry Proanthocyanidins-PANI Nanocomposite for the Detection of Bacteria Associated with Urinary Tract Infections |
| title_full | Cranberry Proanthocyanidins-PANI Nanocomposite for the Detection of Bacteria Associated with Urinary Tract Infections |
| title_fullStr | Cranberry Proanthocyanidins-PANI Nanocomposite for the Detection of Bacteria Associated with Urinary Tract Infections |
| title_full_unstemmed | Cranberry Proanthocyanidins-PANI Nanocomposite for the Detection of Bacteria Associated with Urinary Tract Infections |
| title_short | Cranberry Proanthocyanidins-PANI Nanocomposite for the Detection of Bacteria Associated with Urinary Tract Infections |
| title_sort | cranberry proanthocyanidins pani nanocomposite for the detection of bacteria associated with urinary tract infections |
| topic | proanthocyanidins nanocomposites urinary tract infections extra-intestinal pathogenic <i>Escherichia coli</i> bacteria agglutination |
| url | https://www.mdpi.com/2079-6374/11/6/199 |
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