Capsule-Targeting Depolymerases Derived from <i>Acinetobacter baumannii</i> Prophage Regions
In this study, several different depolymerases encoded in the prophage regions of <i>Acinetobacter baumannii</i> genomes have been bioinformatically predicted and recombinantly produced. The identified depolymerases possessed multi-domain structures and were identical or closely homologo...
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-04-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/9/4971 |
| Summary: | In this study, several different depolymerases encoded in the prophage regions of <i>Acinetobacter baumannii</i> genomes have been bioinformatically predicted and recombinantly produced. The identified depolymerases possessed multi-domain structures and were identical or closely homologous to various proteins encoded in other <i>A. baumannii</i> genomes. This means that prophage-derived depolymerases are widespread, and different bacterial genomes can be the source of proteins with polysaccharide-degrading activities. For two depolymerases, the specificity to capsular polysaccharides (CPSs) of <i>A. baumannii</i> belonging to K1 and K92 capsular types (K types) was determined. The data obtained showed that the prophage-derived depolymerases were glycosidases that cleaved the <i>A. baumannii</i> CPSs by the hydrolytic mechanism to yield monomers and oligomers of the K units. The recombinant proteins with established enzymatic activity significantly reduced the mortality of <i>Galleria mellonella</i> larvae infected with <i>A. baumannii</i> of K1 and K92 capsular types. Therefore, these enzymes can be considered as suitable candidates for the development of new antibacterials against corresponding <i>A. baumannii</i> K types. |
|---|---|
| ISSN: | 1661-6596 1422-0067 |