The MRTF-A/miR-155/SOX1 pathway mediates gastric cancer migration and invasion
Abstract Background Gastric cancer (GC) is the leading cause of death worldwide and is closely related to metastasis. MRTF-A is one of the most well-characterized genetic markers in cancer. However, the mechanism whereby MRTF-A mediate gastric cancer (GC) tumorigenesis is not fully clear. Increasing...
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| Format: | Article |
| Language: | English |
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BMC
2020-07-01
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| Series: | Cancer Cell International |
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| Online Access: | http://link.springer.com/article/10.1186/s12935-020-01395-5 |
| _version_ | 1811220336931241984 |
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| author | Libin Yin Tao Liu Chenyao Li Guoqiang Yan Chao Li Jiantao Zhang Lei Wang |
| author_facet | Libin Yin Tao Liu Chenyao Li Guoqiang Yan Chao Li Jiantao Zhang Lei Wang |
| author_sort | Libin Yin |
| collection | DOAJ |
| description | Abstract Background Gastric cancer (GC) is the leading cause of death worldwide and is closely related to metastasis. MRTF-A is one of the most well-characterized genetic markers in cancer. However, the mechanism whereby MRTF-A mediate gastric cancer (GC) tumorigenesis is not fully clear. Increasing evidence has confirmed that miRNA dysregulation is involved in MRTF-A-mediated tumorigenesis, supporting their potential as therapeutic targets for cancer. Although miR-155 has been reported as an upregulated miRNA, the interplay between miR-155 and MRTF-A-mediated gastric cancer progression remain largely elusive. Methods Real-time PCR was performed to determine miR-155 expression after transfected with MRTF-A encoding plasmids and siRNA. Potential target genes were identified by Western blot and luciferase reporter assay. Chip assay was proved that MRTF-A binds in the promoter region of miR-155. Transwell assay and Scratch-healing migration assay was used to investigate the role of MRTF-A and SOX1 in gastric cancer cell migration and invasion. Results MRTF-A can interact with the miR-155 promoter to promote histone acetylation and RNA polymerase II recruitment via the Wnt-β-catenin pathway. miR-155 promotes gastric cancer cell migration by suppressing SOX1 expressiom by targeting its 3′UTR in vitro and in vivo. MRTF-A inhibited the inhibitory effects of SOX1 on gastric cancer cell migration by promoting the express -ion of miR-155. Conclusion Our data therefore provide important and novel insights into how the MRTF-A/miR-155/SOX1 pathway mediates migration and invasion in GC. |
| first_indexed | 2024-04-12T07:40:13Z |
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| id | doaj.art-661ffeed5cf04b5e9599ecf7911e7bdd |
| institution | Directory Open Access Journal |
| issn | 1475-2867 |
| language | English |
| last_indexed | 2024-04-12T07:40:13Z |
| publishDate | 2020-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Cancer Cell International |
| spelling | doaj.art-661ffeed5cf04b5e9599ecf7911e7bdd2022-12-22T03:41:50ZengBMCCancer Cell International1475-28672020-07-0120111310.1186/s12935-020-01395-5The MRTF-A/miR-155/SOX1 pathway mediates gastric cancer migration and invasionLibin Yin0Tao Liu1Chenyao Li2Guoqiang Yan3Chao Li4Jiantao Zhang5Lei Wang6Department of Colorectal and Anal Surgery, The First Hospital of Jilin UniversityDepartment of Colorectal and Anal Surgery, The First Hospital of Jilin UniversityDepartment of Colorectal and Anal Surgery, The First Hospital of Jilin UniversityDepartment of Colorectal and Anal Surgery, The First Hospital of Jilin UniversityDepartment of Colorectal and Anal Surgery, The First Hospital of Jilin UniversityDepartment of Colorectal and Anal Surgery, The First Hospital of Jilin UniversityDepartment of Colorectal and Anal Surgery, The First Hospital of Jilin UniversityAbstract Background Gastric cancer (GC) is the leading cause of death worldwide and is closely related to metastasis. MRTF-A is one of the most well-characterized genetic markers in cancer. However, the mechanism whereby MRTF-A mediate gastric cancer (GC) tumorigenesis is not fully clear. Increasing evidence has confirmed that miRNA dysregulation is involved in MRTF-A-mediated tumorigenesis, supporting their potential as therapeutic targets for cancer. Although miR-155 has been reported as an upregulated miRNA, the interplay between miR-155 and MRTF-A-mediated gastric cancer progression remain largely elusive. Methods Real-time PCR was performed to determine miR-155 expression after transfected with MRTF-A encoding plasmids and siRNA. Potential target genes were identified by Western blot and luciferase reporter assay. Chip assay was proved that MRTF-A binds in the promoter region of miR-155. Transwell assay and Scratch-healing migration assay was used to investigate the role of MRTF-A and SOX1 in gastric cancer cell migration and invasion. Results MRTF-A can interact with the miR-155 promoter to promote histone acetylation and RNA polymerase II recruitment via the Wnt-β-catenin pathway. miR-155 promotes gastric cancer cell migration by suppressing SOX1 expressiom by targeting its 3′UTR in vitro and in vivo. MRTF-A inhibited the inhibitory effects of SOX1 on gastric cancer cell migration by promoting the express -ion of miR-155. Conclusion Our data therefore provide important and novel insights into how the MRTF-A/miR-155/SOX1 pathway mediates migration and invasion in GC.http://link.springer.com/article/10.1186/s12935-020-01395-5Gastric cancerMRTF-AmiR-155SOX1Migration |
| spellingShingle | Libin Yin Tao Liu Chenyao Li Guoqiang Yan Chao Li Jiantao Zhang Lei Wang The MRTF-A/miR-155/SOX1 pathway mediates gastric cancer migration and invasion Cancer Cell International Gastric cancer MRTF-A miR-155 SOX1 Migration |
| title | The MRTF-A/miR-155/SOX1 pathway mediates gastric cancer migration and invasion |
| title_full | The MRTF-A/miR-155/SOX1 pathway mediates gastric cancer migration and invasion |
| title_fullStr | The MRTF-A/miR-155/SOX1 pathway mediates gastric cancer migration and invasion |
| title_full_unstemmed | The MRTF-A/miR-155/SOX1 pathway mediates gastric cancer migration and invasion |
| title_short | The MRTF-A/miR-155/SOX1 pathway mediates gastric cancer migration and invasion |
| title_sort | mrtf a mir 155 sox1 pathway mediates gastric cancer migration and invasion |
| topic | Gastric cancer MRTF-A miR-155 SOX1 Migration |
| url | http://link.springer.com/article/10.1186/s12935-020-01395-5 |
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