In vitro and in vivo anthelmintic activity of pumpkin seeds and pomegranate peels extracts against Ascaridia galli
Pumpkin seeds (Cucurbita pepo) and Pomegranate peel (Punica granatum) have anthelmintic properties. The aim of this study was to compare the anthelmintic efficacy of pumpkin seeds ethanolic extract and pomegranate peel aqueous extract against Ascaridia galli in vitro and in vivo in Baladi chicks. On...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2018-06-01
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| Series: | Beni-Suef University Journal of Basic and Applied Sciences |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2314853517303918 |
| _version_ | 1818172278552133632 |
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| author | Amer R. Abdel Aziz Mahmoud R. AbouLaila Mohammad Aziz Mosaab A. Omar Khaled Sultan |
| author_facet | Amer R. Abdel Aziz Mahmoud R. AbouLaila Mohammad Aziz Mosaab A. Omar Khaled Sultan |
| author_sort | Amer R. Abdel Aziz |
| collection | DOAJ |
| description | Pumpkin seeds (Cucurbita pepo) and Pomegranate peel (Punica granatum) have anthelmintic properties. The aim of this study was to compare the anthelmintic efficacy of pumpkin seeds ethanolic extract and pomegranate peel aqueous extract against Ascaridia galli in vitro and in vivo in Baladi chicks. On adult worms, the extracts of the two herbs were compared in vitro at concentrations of 25, 50, and 75 mg/ml with fenbendazole at a concentration of 5 mg/ml. Chicks were infected with Ascaridia galli eggs containing second stage larva and treated with 2000 mg/kg of each of the extracts compared with 100 mg/kg fenbendazole. In vitro, all concentrations of pumpkin seed extract and the concentration of 75 mg/ml pomegranate peel extract exhibited a nearly similar effect to fenbendazole. In vivo, the mortality rate of the worms extracted from the 2000 mg/kg pumpkin seeds extract-treated chicken was non-significantly different from that of fenbendazole for 48 h. While pomegranate peels extract exhibited a lower lethal effect than fenbendazole. The anthelmintic efficacy was dependent on time and concentration. The study presented the anthelmintic efficacy of the pumpkin seeds and pomegranate peel extracts on Ascaridia galli. Pumpkin seed extract was more effective than pomegranate peel extract. Future studies to determine the optimal dose to maximize their effectiveness especially for pumpkin seeds as anthelmintic therapeutic are required. Keywords: Pomegranate peel, Pumpkin seeds, Anthelmintic, Ascaridia galli, In vitro, In vivo |
| first_indexed | 2024-12-11T19:10:04Z |
| format | Article |
| id | doaj.art-6621b743ebdd46c1a32d0911691a1853 |
| institution | Directory Open Access Journal |
| issn | 2314-8535 |
| language | English |
| last_indexed | 2024-12-11T19:10:04Z |
| publishDate | 2018-06-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Beni-Suef University Journal of Basic and Applied Sciences |
| spelling | doaj.art-6621b743ebdd46c1a32d0911691a18532022-12-22T00:53:47ZengSpringerOpenBeni-Suef University Journal of Basic and Applied Sciences2314-85352018-06-0172231234In vitro and in vivo anthelmintic activity of pumpkin seeds and pomegranate peels extracts against Ascaridia galliAmer R. Abdel Aziz0Mahmoud R. AbouLaila1Mohammad Aziz2Mosaab A. Omar3Khaled Sultan4Department of Parasitology, Faculty of Veterinary Medicine, Sohag University, 82424 Sohag, EgyptDepartment of Parasitology and Pathology, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, El-Behera, Egypt; Corresponding author.Department of Parasitology, Medical Research Institute, Alexandria University, 21561 Alexandria, EgyptDepartment of Parasitology, Faculty of Veterinary Medicine, South Valley University, 83523 Qena, EgyptDepartment of Parasitology, Faculty of Veterinary Medicine, Kafr El-Sheikh University, 33156 Kafr El-Sheikh, EgyptPumpkin seeds (Cucurbita pepo) and Pomegranate peel (Punica granatum) have anthelmintic properties. The aim of this study was to compare the anthelmintic efficacy of pumpkin seeds ethanolic extract and pomegranate peel aqueous extract against Ascaridia galli in vitro and in vivo in Baladi chicks. On adult worms, the extracts of the two herbs were compared in vitro at concentrations of 25, 50, and 75 mg/ml with fenbendazole at a concentration of 5 mg/ml. Chicks were infected with Ascaridia galli eggs containing second stage larva and treated with 2000 mg/kg of each of the extracts compared with 100 mg/kg fenbendazole. In vitro, all concentrations of pumpkin seed extract and the concentration of 75 mg/ml pomegranate peel extract exhibited a nearly similar effect to fenbendazole. In vivo, the mortality rate of the worms extracted from the 2000 mg/kg pumpkin seeds extract-treated chicken was non-significantly different from that of fenbendazole for 48 h. While pomegranate peels extract exhibited a lower lethal effect than fenbendazole. The anthelmintic efficacy was dependent on time and concentration. The study presented the anthelmintic efficacy of the pumpkin seeds and pomegranate peel extracts on Ascaridia galli. Pumpkin seed extract was more effective than pomegranate peel extract. Future studies to determine the optimal dose to maximize their effectiveness especially for pumpkin seeds as anthelmintic therapeutic are required. Keywords: Pomegranate peel, Pumpkin seeds, Anthelmintic, Ascaridia galli, In vitro, In vivohttp://www.sciencedirect.com/science/article/pii/S2314853517303918 |
| spellingShingle | Amer R. Abdel Aziz Mahmoud R. AbouLaila Mohammad Aziz Mosaab A. Omar Khaled Sultan In vitro and in vivo anthelmintic activity of pumpkin seeds and pomegranate peels extracts against Ascaridia galli Beni-Suef University Journal of Basic and Applied Sciences |
| title | In vitro and in vivo anthelmintic activity of pumpkin seeds and pomegranate peels extracts against Ascaridia galli |
| title_full | In vitro and in vivo anthelmintic activity of pumpkin seeds and pomegranate peels extracts against Ascaridia galli |
| title_fullStr | In vitro and in vivo anthelmintic activity of pumpkin seeds and pomegranate peels extracts against Ascaridia galli |
| title_full_unstemmed | In vitro and in vivo anthelmintic activity of pumpkin seeds and pomegranate peels extracts against Ascaridia galli |
| title_short | In vitro and in vivo anthelmintic activity of pumpkin seeds and pomegranate peels extracts against Ascaridia galli |
| title_sort | in vitro and in vivo anthelmintic activity of pumpkin seeds and pomegranate peels extracts against ascaridia galli |
| url | http://www.sciencedirect.com/science/article/pii/S2314853517303918 |
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