Cell Density-Dependent Fibroblast Growth Factor-2 Signaling Regulates Syndecan-4 Expression in Cultured Vascular Endothelial Cells
Syndecan-4 is a member of the syndecan family of transmembrane heparan sulfate proteoglycans, and is involved in cell protection, proliferation, and the blood coagulation-fibrinolytic system in vascular endothelial cells. Heparan sulfate chains enable fibroblast growth factor-2 (FGF-2) to form a com...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/10/3698 |
| _version_ | 1797567158245392384 |
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| author | Takato Hara Shiori Yabushita Chika Yamamoto Toshiyuki Kaji |
| author_facet | Takato Hara Shiori Yabushita Chika Yamamoto Toshiyuki Kaji |
| author_sort | Takato Hara |
| collection | DOAJ |
| description | Syndecan-4 is a member of the syndecan family of transmembrane heparan sulfate proteoglycans, and is involved in cell protection, proliferation, and the blood coagulation-fibrinolytic system in vascular endothelial cells. Heparan sulfate chains enable fibroblast growth factor-2 (FGF-2) to form a complex with its receptor and to transduce the cell growth signal. In the present study, bovine aortic endothelial cells were cultured, and the intracellular signal pathways that mediate the regulation of syndecan-4 expression in dense and sparse cultures by FGF-2 were analyzed. We demonstrated the cell density-dependent differential regulation of syndecan-4 expression. Specifically, we found that FGF-2 upregulated the synthesis of syndecan-4 in vascular endothelial cells via the MEK1/2-ERK1/2 pathway in dense cell cultures, with only a transcriptional induction of syndecan-4 at a low cell density via the Akt pathway. This study highlights a critical mechanism underlying the regulation of endothelial cell functions by proteoglycans. |
| first_indexed | 2024-03-10T19:37:41Z |
| format | Article |
| id | doaj.art-66255ef2b5ed4b44a21833cf69564f3a |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T19:37:41Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-66255ef2b5ed4b44a21833cf69564f3a2023-11-20T01:34:12ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-05-012110369810.3390/ijms21103698Cell Density-Dependent Fibroblast Growth Factor-2 Signaling Regulates Syndecan-4 Expression in Cultured Vascular Endothelial CellsTakato Hara0Shiori Yabushita1Chika Yamamoto2Toshiyuki Kaji3Department of Environmental Health, Faculty of Pharmaceutical Sciences, Toho University, Funabashi 274-8510, JapanDepartment of Environmental Health, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Noda 278-8510, JapanDepartment of Environmental Health, Faculty of Pharmaceutical Sciences, Toho University, Funabashi 274-8510, JapanDepartment of Environmental Health, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Noda 278-8510, JapanSyndecan-4 is a member of the syndecan family of transmembrane heparan sulfate proteoglycans, and is involved in cell protection, proliferation, and the blood coagulation-fibrinolytic system in vascular endothelial cells. Heparan sulfate chains enable fibroblast growth factor-2 (FGF-2) to form a complex with its receptor and to transduce the cell growth signal. In the present study, bovine aortic endothelial cells were cultured, and the intracellular signal pathways that mediate the regulation of syndecan-4 expression in dense and sparse cultures by FGF-2 were analyzed. We demonstrated the cell density-dependent differential regulation of syndecan-4 expression. Specifically, we found that FGF-2 upregulated the synthesis of syndecan-4 in vascular endothelial cells via the MEK1/2-ERK1/2 pathway in dense cell cultures, with only a transcriptional induction of syndecan-4 at a low cell density via the Akt pathway. This study highlights a critical mechanism underlying the regulation of endothelial cell functions by proteoglycans.https://www.mdpi.com/1422-0067/21/10/3698endothelial cellFGF-2proteoglycansyndecan-4cell density |
| spellingShingle | Takato Hara Shiori Yabushita Chika Yamamoto Toshiyuki Kaji Cell Density-Dependent Fibroblast Growth Factor-2 Signaling Regulates Syndecan-4 Expression in Cultured Vascular Endothelial Cells International Journal of Molecular Sciences endothelial cell FGF-2 proteoglycan syndecan-4 cell density |
| title | Cell Density-Dependent Fibroblast Growth Factor-2 Signaling Regulates Syndecan-4 Expression in Cultured Vascular Endothelial Cells |
| title_full | Cell Density-Dependent Fibroblast Growth Factor-2 Signaling Regulates Syndecan-4 Expression in Cultured Vascular Endothelial Cells |
| title_fullStr | Cell Density-Dependent Fibroblast Growth Factor-2 Signaling Regulates Syndecan-4 Expression in Cultured Vascular Endothelial Cells |
| title_full_unstemmed | Cell Density-Dependent Fibroblast Growth Factor-2 Signaling Regulates Syndecan-4 Expression in Cultured Vascular Endothelial Cells |
| title_short | Cell Density-Dependent Fibroblast Growth Factor-2 Signaling Regulates Syndecan-4 Expression in Cultured Vascular Endothelial Cells |
| title_sort | cell density dependent fibroblast growth factor 2 signaling regulates syndecan 4 expression in cultured vascular endothelial cells |
| topic | endothelial cell FGF-2 proteoglycan syndecan-4 cell density |
| url | https://www.mdpi.com/1422-0067/21/10/3698 |
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