Mechanism of lily bulb and Rehmannia decoction in the treatment of lipopolysaccharide-induced depression-like rats based on metabolomics study and network pharmacology
Context Lily bulb and Rehmannia decoction (LBRD), consisting of Lilium henryi Baker (Liliaceae) and Rehmannia glutinosa (Gaertn) DC (Plantaginaceae), is a specialized traditional Chinese medicine formula for treating depression. However, the underlying mechanisms, especially the relationship between...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2022-12-01
|
| Series: | Pharmaceutical Biology |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/13880209.2022.2121843 |
| _version_ | 1798019755887558656 |
|---|---|
| author | Xiansu Chi Xiaoyan Xue Jin Pan Jiang Wu Huishan Shi Yong Wang Yanting Lu Zhe Zhang Ke Ma |
| author_facet | Xiansu Chi Xiaoyan Xue Jin Pan Jiang Wu Huishan Shi Yong Wang Yanting Lu Zhe Zhang Ke Ma |
| author_sort | Xiansu Chi |
| collection | DOAJ |
| description | Context Lily bulb and Rehmannia decoction (LBRD), consisting of Lilium henryi Baker (Liliaceae) and Rehmannia glutinosa (Gaertn) DC (Plantaginaceae), is a specialized traditional Chinese medicine formula for treating depression. However, the underlying mechanisms, especially the relationship between LBRD efficacy and metabolomics, remains unclear.Objective This study was aimed to investigate the metabolic mechanism of LBRD in treating depression.Materials and methods Network pharmacology was conducted using SwissTargetPrediction, DisGeNET, DrugBank, Metascape, etc., to construct component-target-pathway networks. The depression-like model was induced by intraperitoneal injection with lipopolysaccharide (LPS) (0.3 mg/kg) for 14 consecutive days. After the administration of LBRD (90 g/kg) and fluoxetine (2 mg/kg) for 14 days, we assessed behaviour and the levels of neurotransmitter, inflammatory cytokine and circulating stress hormone. Prefrontal metabolites of rats were detected by using liquid chromatography–mass spectrometry metabolomics method.Results The results of network pharmacology showed that LBRD mainly acted on neurotransmitter and second messenger pathways. Compared to the model group, LBRD significantly ameliorated depressive phenotypes and increased the level of 5-HT (13.4%) and GABA (24.8%), as well as decreased IL-1β (30.7%), IL-6 (32.8%) and TNF-α (26.6%). Followed by LBRD treatment, the main metabolites in prefrontal tissue were contributed to retrograde endocannabinoid signalling, glycerophospholipid metabolism, glycosylphosphatidylinositol-anchor biosynthesis, autophagy signal pathway, etc.Discussion and conclusions LBRD were effective at increasing neurotransmitter, attenuating proinflammatory cytokine and regulating glycerophospholipid metabolism and glutamatergic synapse, thereby ameliorating depressive phenotypes. This research will offer reference for elucidating the metabolomic mechanism underlying novel antidepressant agents contained LBRD formula. |
| first_indexed | 2024-04-11T16:46:04Z |
| format | Article |
| id | doaj.art-6628a62989454a3689dca1834fa87b24 |
| institution | Directory Open Access Journal |
| issn | 1388-0209 1744-5116 |
| language | English |
| last_indexed | 2024-04-11T16:46:04Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Pharmaceutical Biology |
| spelling | doaj.art-6628a62989454a3689dca1834fa87b242022-12-22T04:13:33ZengTaylor & Francis GroupPharmaceutical Biology1388-02091744-51162022-12-016011850186410.1080/13880209.2022.2121843Mechanism of lily bulb and Rehmannia decoction in the treatment of lipopolysaccharide-induced depression-like rats based on metabolomics study and network pharmacologyXiansu Chi0Xiaoyan Xue1Jin Pan2Jiang Wu3Huishan Shi4Yong Wang5Yanting Lu6Zhe Zhang7Ke Ma8Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, PR ChinaShandong Co-Innovation Center of Classic TCM Formula, Shandong University of Traditional Chinese Medicine, Jinan, PR ChinaShandong Co-Innovation Center of Classic TCM Formula, Shandong University of Traditional Chinese Medicine, Jinan, PR ChinaShandong Co-Innovation Center of Classic TCM Formula, Shandong University of Traditional Chinese Medicine, Jinan, PR ChinaShandong Co-Innovation Center of Classic TCM Formula, Shandong University of Traditional Chinese Medicine, Jinan, PR ChinaShandong Co-Innovation Center of Classic TCM Formula, Shandong University of Traditional Chinese Medicine, Jinan, PR ChinaShandong Co-Innovation Center of Classic TCM Formula, Shandong University of Traditional Chinese Medicine, Jinan, PR ChinaShandong Co-Innovation Center of Classic TCM Formula, Shandong University of Traditional Chinese Medicine, Jinan, PR ChinaShandong Co-Innovation Center of Classic TCM Formula, Shandong University of Traditional Chinese Medicine, Jinan, PR ChinaContext Lily bulb and Rehmannia decoction (LBRD), consisting of Lilium henryi Baker (Liliaceae) and Rehmannia glutinosa (Gaertn) DC (Plantaginaceae), is a specialized traditional Chinese medicine formula for treating depression. However, the underlying mechanisms, especially the relationship between LBRD efficacy and metabolomics, remains unclear.Objective This study was aimed to investigate the metabolic mechanism of LBRD in treating depression.Materials and methods Network pharmacology was conducted using SwissTargetPrediction, DisGeNET, DrugBank, Metascape, etc., to construct component-target-pathway networks. The depression-like model was induced by intraperitoneal injection with lipopolysaccharide (LPS) (0.3 mg/kg) for 14 consecutive days. After the administration of LBRD (90 g/kg) and fluoxetine (2 mg/kg) for 14 days, we assessed behaviour and the levels of neurotransmitter, inflammatory cytokine and circulating stress hormone. Prefrontal metabolites of rats were detected by using liquid chromatography–mass spectrometry metabolomics method.Results The results of network pharmacology showed that LBRD mainly acted on neurotransmitter and second messenger pathways. Compared to the model group, LBRD significantly ameliorated depressive phenotypes and increased the level of 5-HT (13.4%) and GABA (24.8%), as well as decreased IL-1β (30.7%), IL-6 (32.8%) and TNF-α (26.6%). Followed by LBRD treatment, the main metabolites in prefrontal tissue were contributed to retrograde endocannabinoid signalling, glycerophospholipid metabolism, glycosylphosphatidylinositol-anchor biosynthesis, autophagy signal pathway, etc.Discussion and conclusions LBRD were effective at increasing neurotransmitter, attenuating proinflammatory cytokine and regulating glycerophospholipid metabolism and glutamatergic synapse, thereby ameliorating depressive phenotypes. This research will offer reference for elucidating the metabolomic mechanism underlying novel antidepressant agents contained LBRD formula.https://www.tandfonline.com/doi/10.1080/13880209.2022.2121843Traditional Chinese medicine formuladepressive disordermedial prefrontal cortexmetabolic components |
| spellingShingle | Xiansu Chi Xiaoyan Xue Jin Pan Jiang Wu Huishan Shi Yong Wang Yanting Lu Zhe Zhang Ke Ma Mechanism of lily bulb and Rehmannia decoction in the treatment of lipopolysaccharide-induced depression-like rats based on metabolomics study and network pharmacology Pharmaceutical Biology Traditional Chinese medicine formula depressive disorder medial prefrontal cortex metabolic components |
| title | Mechanism of lily bulb and Rehmannia decoction in the treatment of lipopolysaccharide-induced depression-like rats based on metabolomics study and network pharmacology |
| title_full | Mechanism of lily bulb and Rehmannia decoction in the treatment of lipopolysaccharide-induced depression-like rats based on metabolomics study and network pharmacology |
| title_fullStr | Mechanism of lily bulb and Rehmannia decoction in the treatment of lipopolysaccharide-induced depression-like rats based on metabolomics study and network pharmacology |
| title_full_unstemmed | Mechanism of lily bulb and Rehmannia decoction in the treatment of lipopolysaccharide-induced depression-like rats based on metabolomics study and network pharmacology |
| title_short | Mechanism of lily bulb and Rehmannia decoction in the treatment of lipopolysaccharide-induced depression-like rats based on metabolomics study and network pharmacology |
| title_sort | mechanism of lily bulb and rehmannia decoction in the treatment of lipopolysaccharide induced depression like rats based on metabolomics study and network pharmacology |
| topic | Traditional Chinese medicine formula depressive disorder medial prefrontal cortex metabolic components |
| url | https://www.tandfonline.com/doi/10.1080/13880209.2022.2121843 |
| work_keys_str_mv | AT xiansuchi mechanismoflilybulbandrehmanniadecoctioninthetreatmentoflipopolysaccharideinduceddepressionlikeratsbasedonmetabolomicsstudyandnetworkpharmacology AT xiaoyanxue mechanismoflilybulbandrehmanniadecoctioninthetreatmentoflipopolysaccharideinduceddepressionlikeratsbasedonmetabolomicsstudyandnetworkpharmacology AT jinpan mechanismoflilybulbandrehmanniadecoctioninthetreatmentoflipopolysaccharideinduceddepressionlikeratsbasedonmetabolomicsstudyandnetworkpharmacology AT jiangwu mechanismoflilybulbandrehmanniadecoctioninthetreatmentoflipopolysaccharideinduceddepressionlikeratsbasedonmetabolomicsstudyandnetworkpharmacology AT huishanshi mechanismoflilybulbandrehmanniadecoctioninthetreatmentoflipopolysaccharideinduceddepressionlikeratsbasedonmetabolomicsstudyandnetworkpharmacology AT yongwang mechanismoflilybulbandrehmanniadecoctioninthetreatmentoflipopolysaccharideinduceddepressionlikeratsbasedonmetabolomicsstudyandnetworkpharmacology AT yantinglu mechanismoflilybulbandrehmanniadecoctioninthetreatmentoflipopolysaccharideinduceddepressionlikeratsbasedonmetabolomicsstudyandnetworkpharmacology AT zhezhang mechanismoflilybulbandrehmanniadecoctioninthetreatmentoflipopolysaccharideinduceddepressionlikeratsbasedonmetabolomicsstudyandnetworkpharmacology AT kema mechanismoflilybulbandrehmanniadecoctioninthetreatmentoflipopolysaccharideinduceddepressionlikeratsbasedonmetabolomicsstudyandnetworkpharmacology |