Bacterial bioburden and community structure of potable water used in the International Space Station
Abstract The control of microbes in manned spaceflight is essential to reducing the risk of infection and maintaining crew health. The primary issue is ensuring the safety of a potable water system, where simultaneous monitoring of microbial abundance and community structure is needed. In this paper...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-09-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-19320-3 |
| _version_ | 1811253741892927488 |
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| author | Tomoaki Ichijo Kimiko Uchii Kazuma Sekimoto Takashi Minakami Takashi Sugita Masao Nasu Takashi Yamazaki |
| author_facet | Tomoaki Ichijo Kimiko Uchii Kazuma Sekimoto Takashi Minakami Takashi Sugita Masao Nasu Takashi Yamazaki |
| author_sort | Tomoaki Ichijo |
| collection | DOAJ |
| description | Abstract The control of microbes in manned spaceflight is essential to reducing the risk of infection and maintaining crew health. The primary issue is ensuring the safety of a potable water system, where simultaneous monitoring of microbial abundance and community structure is needed. In this paper, we develop a flow cytometry-based counting protocol targeting cellular flavin autofluorescence as a tool for rapid monitoring of bacterial cells in water. This was successfully applied to estimate the bacterial bioburden in the potable water collected from the International Space Station. We also demonstrate the efficacy of the MinION nanopore sequencer in rapidly characterizing bacterial community structure and identifying the dominant species. These monitoring protocols' rapidity and cost effectiveness would contribute to developing sustainable real-time surveillance of potable water in spaceflight. |
| first_indexed | 2024-04-12T16:55:44Z |
| format | Article |
| id | doaj.art-66298aadee6f44bd9b684de6e27955df |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-12T16:55:44Z |
| publishDate | 2022-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-66298aadee6f44bd9b684de6e27955df2022-12-22T03:24:15ZengNature PortfolioScientific Reports2045-23222022-09-011211910.1038/s41598-022-19320-3Bacterial bioburden and community structure of potable water used in the International Space StationTomoaki Ichijo0Kimiko Uchii1Kazuma Sekimoto2Takashi Minakami3Takashi Sugita4Masao Nasu5Takashi Yamazaki6Faculty of Health and Nutrition, Osaka Shoin Women’s UniversityFaculty of Pharmacy, Osaka Ohtani UniversityParticle Counter Division, RION Co., Ltd.Particle Counter Division, RION Co., Ltd.Department of Microbiology, Meiji Pharmaceutical UniversityGraduate School of Pharmaceutical Sciences, Osaka Ohtani UniversityGeneral Medical Education and Research Center, Teikyo UniversityAbstract The control of microbes in manned spaceflight is essential to reducing the risk of infection and maintaining crew health. The primary issue is ensuring the safety of a potable water system, where simultaneous monitoring of microbial abundance and community structure is needed. In this paper, we develop a flow cytometry-based counting protocol targeting cellular flavin autofluorescence as a tool for rapid monitoring of bacterial cells in water. This was successfully applied to estimate the bacterial bioburden in the potable water collected from the International Space Station. We also demonstrate the efficacy of the MinION nanopore sequencer in rapidly characterizing bacterial community structure and identifying the dominant species. These monitoring protocols' rapidity and cost effectiveness would contribute to developing sustainable real-time surveillance of potable water in spaceflight.https://doi.org/10.1038/s41598-022-19320-3 |
| spellingShingle | Tomoaki Ichijo Kimiko Uchii Kazuma Sekimoto Takashi Minakami Takashi Sugita Masao Nasu Takashi Yamazaki Bacterial bioburden and community structure of potable water used in the International Space Station Scientific Reports |
| title | Bacterial bioburden and community structure of potable water used in the International Space Station |
| title_full | Bacterial bioburden and community structure of potable water used in the International Space Station |
| title_fullStr | Bacterial bioburden and community structure of potable water used in the International Space Station |
| title_full_unstemmed | Bacterial bioburden and community structure of potable water used in the International Space Station |
| title_short | Bacterial bioburden and community structure of potable water used in the International Space Station |
| title_sort | bacterial bioburden and community structure of potable water used in the international space station |
| url | https://doi.org/10.1038/s41598-022-19320-3 |
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