Inhibition of tick-borne encephalitis virus in cell cultures by ribavirin
Tick-borne encephalitis virus (TBEV) belonging to arboviruses is a major member of zoonotic pathogens. TBEV infection causes severe human encephalitis without specific antiviral drugs. Due to its use of antiviral drug against a wide range of viruses, we investigated antiviral effect of ribavirin aga...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-06-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1182798/full |
| _version_ | 1797806302715445248 |
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| author | Wan-Da Tang Hai-Lin Tang Hao-Ran Peng Rui-Wen Ren Ping Zhao Lan-Juan Zhao |
| author_facet | Wan-Da Tang Hai-Lin Tang Hao-Ran Peng Rui-Wen Ren Ping Zhao Lan-Juan Zhao |
| author_sort | Wan-Da Tang |
| collection | DOAJ |
| description | Tick-borne encephalitis virus (TBEV) belonging to arboviruses is a major member of zoonotic pathogens. TBEV infection causes severe human encephalitis without specific antiviral drugs. Due to its use of antiviral drug against a wide range of viruses, we investigated antiviral effect of ribavirin against TBEV in susceptible human cell lines A549 and SH-SY5Y. Ribavirin displayed minor cytotoxicity on multiple cell lines. Ribavirin obviously impaired TBEV replication and protected the infected cells from cytopathic effect. Importantly, ribavirin markedly inhibited TBEV propagation, as evidenced by impairment of TBEV production and viral RNA replication. Treatment with ribavirin (co-treatment and post-treatment) led to a dose-dependent reduction in TBEV titers as well as the viral RNA levels. Antiviral protein myxovirus resistance A mRNA expression was significantly up-regulated and signal transducer and activator of transcription 3 was activated in TBEV-infected A549 cells upon the ribavirin treatment. Induction of inflammatory cytokine tumor necrosis factor alpha by TBEV was decreased in A549 cells with the treatment of ribavirin, whereas interleukin 1 beta release appeared to be unaffected. These results suggest that ribavirin might represent a promising safe and effective antiviral drug against TBEV. |
| first_indexed | 2024-03-13T06:05:18Z |
| format | Article |
| id | doaj.art-6629d5d6cedb424ca3c92b0efa65b3b2 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-13T06:05:18Z |
| publishDate | 2023-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-6629d5d6cedb424ca3c92b0efa65b3b22023-06-12T04:21:47ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-06-011410.3389/fmicb.2023.11827981182798Inhibition of tick-borne encephalitis virus in cell cultures by ribavirinWan-Da Tang0Hai-Lin Tang1Hao-Ran Peng2Rui-Wen Ren3Ping Zhao4Lan-Juan Zhao5Department of Microbiology, Faculty of Naval Medicine, Naval Medical University, Shanghai, ChinaDepartment of Microbiology, Faculty of Naval Medicine, Naval Medical University, Shanghai, ChinaDepartment of Microbiology, Faculty of Naval Medicine, Naval Medical University, Shanghai, ChinaCenter for Disease Control and Prevention of Southern Theater Command, Guangzhou, ChinaDepartment of Microbiology, Faculty of Naval Medicine, Naval Medical University, Shanghai, ChinaDepartment of Microbiology, Faculty of Naval Medicine, Naval Medical University, Shanghai, ChinaTick-borne encephalitis virus (TBEV) belonging to arboviruses is a major member of zoonotic pathogens. TBEV infection causes severe human encephalitis without specific antiviral drugs. Due to its use of antiviral drug against a wide range of viruses, we investigated antiviral effect of ribavirin against TBEV in susceptible human cell lines A549 and SH-SY5Y. Ribavirin displayed minor cytotoxicity on multiple cell lines. Ribavirin obviously impaired TBEV replication and protected the infected cells from cytopathic effect. Importantly, ribavirin markedly inhibited TBEV propagation, as evidenced by impairment of TBEV production and viral RNA replication. Treatment with ribavirin (co-treatment and post-treatment) led to a dose-dependent reduction in TBEV titers as well as the viral RNA levels. Antiviral protein myxovirus resistance A mRNA expression was significantly up-regulated and signal transducer and activator of transcription 3 was activated in TBEV-infected A549 cells upon the ribavirin treatment. Induction of inflammatory cytokine tumor necrosis factor alpha by TBEV was decreased in A549 cells with the treatment of ribavirin, whereas interleukin 1 beta release appeared to be unaffected. These results suggest that ribavirin might represent a promising safe and effective antiviral drug against TBEV.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1182798/fulltick-borne encephalitis virusribavirinmyxovirus resistance Asignal transducer and activator of transcription 3tumor necrosis factor alpha |
| spellingShingle | Wan-Da Tang Hai-Lin Tang Hao-Ran Peng Rui-Wen Ren Ping Zhao Lan-Juan Zhao Inhibition of tick-borne encephalitis virus in cell cultures by ribavirin Frontiers in Microbiology tick-borne encephalitis virus ribavirin myxovirus resistance A signal transducer and activator of transcription 3 tumor necrosis factor alpha |
| title | Inhibition of tick-borne encephalitis virus in cell cultures by ribavirin |
| title_full | Inhibition of tick-borne encephalitis virus in cell cultures by ribavirin |
| title_fullStr | Inhibition of tick-borne encephalitis virus in cell cultures by ribavirin |
| title_full_unstemmed | Inhibition of tick-borne encephalitis virus in cell cultures by ribavirin |
| title_short | Inhibition of tick-borne encephalitis virus in cell cultures by ribavirin |
| title_sort | inhibition of tick borne encephalitis virus in cell cultures by ribavirin |
| topic | tick-borne encephalitis virus ribavirin myxovirus resistance A signal transducer and activator of transcription 3 tumor necrosis factor alpha |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1182798/full |
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