Chronic hepatitis B in children carried out of the hemochromatosis gene HFE
Purpose: to establish the frequency and clinical significance of mutant variations of the HFE gene polymorphism in chronic hepatitis B (CHB) in children with iron overload syndrome (IOS).Materials and methods: 60 children with chronic hepatitis B with iron overload syndrome (IOS) were examined. When...
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| Format: | Article |
| Language: | Russian |
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Journal Infectology
2021-07-01
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| Series: | Журнал инфектологии |
| Subjects: | |
| Online Access: | https://journal.niidi.ru/jofin/article/view/1213 |
| _version_ | 1797702630152077312 |
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| author | F. I. Inoyatova Kh. M. Kadyrkhodzhayeva G. Z. Inogamova N. A. Ikramova F. G. Abdullayeva N. K. Valiyeva A. Kh. Akhmedova |
| author_facet | F. I. Inoyatova Kh. M. Kadyrkhodzhayeva G. Z. Inogamova N. A. Ikramova F. G. Abdullayeva N. K. Valiyeva A. Kh. Akhmedova |
| author_sort | F. I. Inoyatova |
| collection | DOAJ |
| description | Purpose: to establish the frequency and clinical significance of mutant variations of the HFE gene polymorphism in chronic hepatitis B (CHB) in children with iron overload syndrome (IOS).Materials and methods: 60 children with chronic hepatitis B with iron overload syndrome (IOS) were examined. When distributing children into groups, we took into account the criteria we developed for assessing the degree of life expectancy in children with CHB: CST>0.5 – mild degree of life expectancy (43,3% of children), CST˃0.5 – mild degree of life expectancy (43,3% of children), CST˂0,5 – moderate severity of life expectancy (31,7% of children) and CST˂0,2 – severe degree of SPL (25,0%). Virological verification of HBV was performed by ELISA and PCR. Using PCR Real Time and molecular genetic analysis, HFE gene C282Y, H63D, S65C mutations were detected from amplified DNA using the PRONTO Hemochromatosis reagent kit (Israel). The transferrin saturation coefficient (CST) was calculated using the formula CST = sTfR / log10.Ft. Results:Results: The study of the hemochromatosis gene HFE showed that the overwhelming majority (84,0%) of children with CHB with IOS were carriers of heterozygous, phenotypically different, mutant types. And only 16,0% of sick children were homozites of the wild (normal) HFE gene. Analysis of the phenotypic polymorphism of the hemochromatosis gene HFE revealed the presence of three point heterozygous mutations: H63D, S65C and combined variations in H63D / S65C, the latter of which is associated with severe forms of CHB and severe IOS.Conclusion. Children with CHB with IOS are characterized by a high incidence of heterozygous mutations in the HFE gene, the phenotypic manifestations of which were S65C, H63D, H63D / S65C. The comparability of the heterozygous combined mutation H63D / S65C with severe forms of CHB and a severe degree of IOS gives grounds to consider this phenotype of the HFE gene as a factor in the progression of the disease. |
| first_indexed | 2024-03-12T04:52:52Z |
| format | Article |
| id | doaj.art-662b4d0b0fd24c2795da116453da02fb |
| institution | Directory Open Access Journal |
| issn | 2072-6732 |
| language | Russian |
| last_indexed | 2024-03-12T04:52:52Z |
| publishDate | 2021-07-01 |
| publisher | Journal Infectology |
| record_format | Article |
| series | Журнал инфектологии |
| spelling | doaj.art-662b4d0b0fd24c2795da116453da02fb2023-09-03T09:18:30ZrusJournal InfectologyЖурнал инфектологии2072-67322021-07-0113210811410.22625/2072-6732-2021-13-2-108-114915Chronic hepatitis B in children carried out of the hemochromatosis gene HFEF. I. Inoyatova0Kh. M. Kadyrkhodzhayeva1G. Z. Inogamova2N. A. Ikramova3F. G. Abdullayeva4N. K. Valiyeva5A. Kh. Akhmedova6Республиканский специализированный научно-практический медицинский центр педиатрииРеспубликанский специализированный научно-практический медицинский центр педиатрииРеспубликанский специализированный научно-практический медицинский центр педиатрииРеспубликанский специализированный научно-практический медицинский центр педиатрииРеспубликанский специализированный научно-практический медицинский центр педиатрииРеспубликанский специализированный научно-практический медицинский центр педиатрииРеспубликанский специализированный научно-практический медицинский центр педиатрииPurpose: to establish the frequency and clinical significance of mutant variations of the HFE gene polymorphism in chronic hepatitis B (CHB) in children with iron overload syndrome (IOS).Materials and methods: 60 children with chronic hepatitis B with iron overload syndrome (IOS) were examined. When distributing children into groups, we took into account the criteria we developed for assessing the degree of life expectancy in children with CHB: CST>0.5 – mild degree of life expectancy (43,3% of children), CST˃0.5 – mild degree of life expectancy (43,3% of children), CST˂0,5 – moderate severity of life expectancy (31,7% of children) and CST˂0,2 – severe degree of SPL (25,0%). Virological verification of HBV was performed by ELISA and PCR. Using PCR Real Time and molecular genetic analysis, HFE gene C282Y, H63D, S65C mutations were detected from amplified DNA using the PRONTO Hemochromatosis reagent kit (Israel). The transferrin saturation coefficient (CST) was calculated using the formula CST = sTfR / log10.Ft. Results:Results: The study of the hemochromatosis gene HFE showed that the overwhelming majority (84,0%) of children with CHB with IOS were carriers of heterozygous, phenotypically different, mutant types. And only 16,0% of sick children were homozites of the wild (normal) HFE gene. Analysis of the phenotypic polymorphism of the hemochromatosis gene HFE revealed the presence of three point heterozygous mutations: H63D, S65C and combined variations in H63D / S65C, the latter of which is associated with severe forms of CHB and severe IOS.Conclusion. Children with CHB with IOS are characterized by a high incidence of heterozygous mutations in the HFE gene, the phenotypic manifestations of which were S65C, H63D, H63D / S65C. The comparability of the heterozygous combined mutation H63D / S65C with severe forms of CHB and a severe degree of IOS gives grounds to consider this phenotype of the HFE gene as a factor in the progression of the disease.https://journal.niidi.ru/jofin/article/view/1213детихронический гепатит всиндром перегрузки железомген гемахроматоза hfe |
| spellingShingle | F. I. Inoyatova Kh. M. Kadyrkhodzhayeva G. Z. Inogamova N. A. Ikramova F. G. Abdullayeva N. K. Valiyeva A. Kh. Akhmedova Chronic hepatitis B in children carried out of the hemochromatosis gene HFE Журнал инфектологии дети хронический гепатит в синдром перегрузки железом ген гемахроматоза hfe |
| title | Chronic hepatitis B in children carried out of the hemochromatosis gene HFE |
| title_full | Chronic hepatitis B in children carried out of the hemochromatosis gene HFE |
| title_fullStr | Chronic hepatitis B in children carried out of the hemochromatosis gene HFE |
| title_full_unstemmed | Chronic hepatitis B in children carried out of the hemochromatosis gene HFE |
| title_short | Chronic hepatitis B in children carried out of the hemochromatosis gene HFE |
| title_sort | chronic hepatitis b in children carried out of the hemochromatosis gene hfe |
| topic | дети хронический гепатит в синдром перегрузки железом ген гемахроматоза hfe |
| url | https://journal.niidi.ru/jofin/article/view/1213 |
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