High‐dose administration of purified cyanidin‐3‐glucose or a blackberry extract causes improved mitochondrial function but reduced content in 3T3‐L1 adipocytes
Abstract Berry consumption shows antiobesity and antidiabetic effects in animals and humans. Anthocyanin or berry extracts increase insulin sensitivity, reduce lipid droplet formation, and cause a white‐to‐brite phenotypic switch of adipocytes in vitro. The objective of this study was to investigate...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-06-01
|
| Series: | Food Frontiers |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/fft2.139 |
| _version_ | 1811345251382591488 |
|---|---|
| author | Patrick Solverson George P. Albaugh Dawn J. Harrison Dave L. Luthria David J. Baer Janet A. Novotny |
| author_facet | Patrick Solverson George P. Albaugh Dawn J. Harrison Dave L. Luthria David J. Baer Janet A. Novotny |
| author_sort | Patrick Solverson |
| collection | DOAJ |
| description | Abstract Berry consumption shows antiobesity and antidiabetic effects in animals and humans. Anthocyanin or berry extracts increase insulin sensitivity, reduce lipid droplet formation, and cause a white‐to‐brite phenotypic switch of adipocytes in vitro. The objective of this study was to investigate effects of cyanidin‐3‐glucoside (C3G) or blackberry extract (BBE) on cellular respiration of a fatty acid substrate in differentiated adipocytes. In separate experiments, 3T3‐L1 adipocytes were treated with 0, 150 nM, or 50 μM purified C3G ‐or‐ 0, 150 nM, 25 μM, or 50 μM C3G‐equivalents from a BBE. Baseline, state‐4, state‐3, and uncoupled respiration states were created experimentally and measured by high‐resolution respirometry. Respiration rate within each state was corrected for mitochondrial content determined by PCR. Mitochondrial content was significantly reduced with high‐dose C3G and the two high‐dose BBE. The high‐dose C3G caused a significant increase in respiration rate for baseline, state‐4, state‐3, and uncoupled states compared to control and physiologic dose C3G. The intermediate‐high dose (25 μM) BBE also significantly increased respiration rate for the same states compared to control and other treatment levels of BBE; this effect was not observed at the high‐dose (50 μM) BBE. Administration of high‐dose purified C3G or BBE decreased mitochondrial content but enhanced cellular respiration on a fatty acid substrate in differentiated 3T3‐L1 adipocytes across several mitochondrial states. We did not observe a phenotypic switch to brite adipocytes like others, but our empirical findings in cellular respiration on a fatty acid substrate support the antiobesity effects associated with anthocyanins and berries. |
| first_indexed | 2024-04-13T20:00:26Z |
| format | Article |
| id | doaj.art-662b90194dda410cbe694109319fe962 |
| institution | Directory Open Access Journal |
| issn | 2643-8429 |
| language | English |
| last_indexed | 2024-04-13T20:00:26Z |
| publishDate | 2022-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Food Frontiers |
| spelling | doaj.art-662b90194dda410cbe694109319fe9622022-12-22T02:32:13ZengWileyFood Frontiers2643-84292022-06-013227628410.1002/fft2.139High‐dose administration of purified cyanidin‐3‐glucose or a blackberry extract causes improved mitochondrial function but reduced content in 3T3‐L1 adipocytesPatrick Solverson0George P. Albaugh1Dawn J. Harrison2Dave L. Luthria3David J. Baer4Janet A. Novotny5Department of Nutrition and Exercise Physiology Elson S. Floyd College of Medicine Washington State University Spokane Washington USAUSDA ARS Beltsville Human Nutrition Research Center Beltsville Maryland USAUSDA ARS Beltsville Human Nutrition Research Center Beltsville Maryland USAUSDA ARS Beltsville Human Nutrition Research Center Beltsville Maryland USAUSDA ARS Beltsville Human Nutrition Research Center Beltsville Maryland USAUSDA ARS Beltsville Human Nutrition Research Center Beltsville Maryland USAAbstract Berry consumption shows antiobesity and antidiabetic effects in animals and humans. Anthocyanin or berry extracts increase insulin sensitivity, reduce lipid droplet formation, and cause a white‐to‐brite phenotypic switch of adipocytes in vitro. The objective of this study was to investigate effects of cyanidin‐3‐glucoside (C3G) or blackberry extract (BBE) on cellular respiration of a fatty acid substrate in differentiated adipocytes. In separate experiments, 3T3‐L1 adipocytes were treated with 0, 150 nM, or 50 μM purified C3G ‐or‐ 0, 150 nM, 25 μM, or 50 μM C3G‐equivalents from a BBE. Baseline, state‐4, state‐3, and uncoupled respiration states were created experimentally and measured by high‐resolution respirometry. Respiration rate within each state was corrected for mitochondrial content determined by PCR. Mitochondrial content was significantly reduced with high‐dose C3G and the two high‐dose BBE. The high‐dose C3G caused a significant increase in respiration rate for baseline, state‐4, state‐3, and uncoupled states compared to control and physiologic dose C3G. The intermediate‐high dose (25 μM) BBE also significantly increased respiration rate for the same states compared to control and other treatment levels of BBE; this effect was not observed at the high‐dose (50 μM) BBE. Administration of high‐dose purified C3G or BBE decreased mitochondrial content but enhanced cellular respiration on a fatty acid substrate in differentiated 3T3‐L1 adipocytes across several mitochondrial states. We did not observe a phenotypic switch to brite adipocytes like others, but our empirical findings in cellular respiration on a fatty acid substrate support the antiobesity effects associated with anthocyanins and berries.https://doi.org/10.1002/fft2.139adipocytesberry anthocyaninscellular respirationdiabetesobesity |
| spellingShingle | Patrick Solverson George P. Albaugh Dawn J. Harrison Dave L. Luthria David J. Baer Janet A. Novotny High‐dose administration of purified cyanidin‐3‐glucose or a blackberry extract causes improved mitochondrial function but reduced content in 3T3‐L1 adipocytes Food Frontiers adipocytes berry anthocyanins cellular respiration diabetes obesity |
| title | High‐dose administration of purified cyanidin‐3‐glucose or a blackberry extract causes improved mitochondrial function but reduced content in 3T3‐L1 adipocytes |
| title_full | High‐dose administration of purified cyanidin‐3‐glucose or a blackberry extract causes improved mitochondrial function but reduced content in 3T3‐L1 adipocytes |
| title_fullStr | High‐dose administration of purified cyanidin‐3‐glucose or a blackberry extract causes improved mitochondrial function but reduced content in 3T3‐L1 adipocytes |
| title_full_unstemmed | High‐dose administration of purified cyanidin‐3‐glucose or a blackberry extract causes improved mitochondrial function but reduced content in 3T3‐L1 adipocytes |
| title_short | High‐dose administration of purified cyanidin‐3‐glucose or a blackberry extract causes improved mitochondrial function but reduced content in 3T3‐L1 adipocytes |
| title_sort | high dose administration of purified cyanidin 3 glucose or a blackberry extract causes improved mitochondrial function but reduced content in 3t3 l1 adipocytes |
| topic | adipocytes berry anthocyanins cellular respiration diabetes obesity |
| url | https://doi.org/10.1002/fft2.139 |
| work_keys_str_mv | AT patricksolverson highdoseadministrationofpurifiedcyanidin3glucoseorablackberryextractcausesimprovedmitochondrialfunctionbutreducedcontentin3t3l1adipocytes AT georgepalbaugh highdoseadministrationofpurifiedcyanidin3glucoseorablackberryextractcausesimprovedmitochondrialfunctionbutreducedcontentin3t3l1adipocytes AT dawnjharrison highdoseadministrationofpurifiedcyanidin3glucoseorablackberryextractcausesimprovedmitochondrialfunctionbutreducedcontentin3t3l1adipocytes AT davelluthria highdoseadministrationofpurifiedcyanidin3glucoseorablackberryextractcausesimprovedmitochondrialfunctionbutreducedcontentin3t3l1adipocytes AT davidjbaer highdoseadministrationofpurifiedcyanidin3glucoseorablackberryextractcausesimprovedmitochondrialfunctionbutreducedcontentin3t3l1adipocytes AT janetanovotny highdoseadministrationofpurifiedcyanidin3glucoseorablackberryextractcausesimprovedmitochondrialfunctionbutreducedcontentin3t3l1adipocytes |