The Cytosolic Acetoacetyl-CoA Thiolase TaAACT1 Is Required for Defense against <i>Fusarium pseudograminearum</i> in Wheat
<i>Fusarium pseudograminearum</i> is a major pathogen for the destructive disease Fusarium crown rot (FCR) of wheat (<i>Triticum aestivum</i>). The cytosolic Acetoacetyl-CoA thiolase II (AACT) is the first catalytic enzyme in the mevalonate pathway that biosynthesizes isopren...
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MDPI AG
2023-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/7/6165 |
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| author | Feng Xiong Xiuliang Zhu Changsha Luo Zhixiang Liu Zengyan Zhang |
| author_facet | Feng Xiong Xiuliang Zhu Changsha Luo Zhixiang Liu Zengyan Zhang |
| author_sort | Feng Xiong |
| collection | DOAJ |
| description | <i>Fusarium pseudograminearum</i> is a major pathogen for the destructive disease Fusarium crown rot (FCR) of wheat (<i>Triticum aestivum</i>). The cytosolic Acetoacetyl-CoA thiolase II (AACT) is the first catalytic enzyme in the mevalonate pathway that biosynthesizes isoprenoids in plants. However, there has been no investigation of wheat cytosolic AACT genes in defense against pathogens including <i>Fusarium pseudograminearum</i>. Herein, we identified a cytosolic AACT-encoding gene from wheat, named <i>TaAACT1</i>, and demonstrated its positively regulatory role in the wheat defense response to <i>F. pseudograminearum</i>. One haplotype of <i>TaAACT1</i> in analyzed wheat genotypes was associated with wheat resistance to FCR. The <i>TaAACT1</i> transcript level was elevated after <i>F. pseudograminearum</i> infection, and was higher in FCR-resistant wheat genotypes than in susceptible wheat genotypes. Functional analysis indicated that knock down of <i>TaAACT1</i> impaired resistance against <i>F. pseudograminearum</i> and reduced the expression of downstream defense genes in wheat. TaAACT1 protein was verified to localize in the cytosol of wheat cells. <i>TaAACT1</i> and its modulated defense genes were rapidly responsive to exogenous jasmonate treatment. Collectively, TaAACT1 contributes to resistance to <i>F. pseudograminearum</i> through upregulating the expression of defense genes in wheat. This study sheds new light on the molecular mechanisms underlying wheat defense against FCR. |
| first_indexed | 2024-03-11T05:35:49Z |
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| last_indexed | 2024-03-11T05:35:49Z |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-662f1459408f47dda3e015f659d49ddd2023-11-17T16:47:25ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-03-01247616510.3390/ijms24076165The Cytosolic Acetoacetyl-CoA Thiolase TaAACT1 Is Required for Defense against <i>Fusarium pseudograminearum</i> in WheatFeng Xiong0Xiuliang Zhu1Changsha Luo2Zhixiang Liu3Zengyan Zhang4Hunan Provincial Key Laboratory of Forestry Biotechnology, College of Life Science and Technology, Central South University of Forestry and Technology, Changsha 410004, ChinaThe National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaHunan Provincial Key Laboratory of Forestry Biotechnology, College of Life Science and Technology, Central South University of Forestry and Technology, Changsha 410004, ChinaHunan Provincial Key Laboratory of Forestry Biotechnology, College of Life Science and Technology, Central South University of Forestry and Technology, Changsha 410004, ChinaThe National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China<i>Fusarium pseudograminearum</i> is a major pathogen for the destructive disease Fusarium crown rot (FCR) of wheat (<i>Triticum aestivum</i>). The cytosolic Acetoacetyl-CoA thiolase II (AACT) is the first catalytic enzyme in the mevalonate pathway that biosynthesizes isoprenoids in plants. However, there has been no investigation of wheat cytosolic AACT genes in defense against pathogens including <i>Fusarium pseudograminearum</i>. Herein, we identified a cytosolic AACT-encoding gene from wheat, named <i>TaAACT1</i>, and demonstrated its positively regulatory role in the wheat defense response to <i>F. pseudograminearum</i>. One haplotype of <i>TaAACT1</i> in analyzed wheat genotypes was associated with wheat resistance to FCR. The <i>TaAACT1</i> transcript level was elevated after <i>F. pseudograminearum</i> infection, and was higher in FCR-resistant wheat genotypes than in susceptible wheat genotypes. Functional analysis indicated that knock down of <i>TaAACT1</i> impaired resistance against <i>F. pseudograminearum</i> and reduced the expression of downstream defense genes in wheat. TaAACT1 protein was verified to localize in the cytosol of wheat cells. <i>TaAACT1</i> and its modulated defense genes were rapidly responsive to exogenous jasmonate treatment. Collectively, TaAACT1 contributes to resistance to <i>F. pseudograminearum</i> through upregulating the expression of defense genes in wheat. This study sheds new light on the molecular mechanisms underlying wheat defense against FCR.https://www.mdpi.com/1422-0067/24/7/6165cytosolic acetoacetyl-CoA thiolase<i>Fusarium pseudograminearum</i>jasmonateplant defensewheat (<i>Triticum aestivum</i>) |
| spellingShingle | Feng Xiong Xiuliang Zhu Changsha Luo Zhixiang Liu Zengyan Zhang The Cytosolic Acetoacetyl-CoA Thiolase TaAACT1 Is Required for Defense against <i>Fusarium pseudograminearum</i> in Wheat International Journal of Molecular Sciences cytosolic acetoacetyl-CoA thiolase <i>Fusarium pseudograminearum</i> jasmonate plant defense wheat (<i>Triticum aestivum</i>) |
| title | The Cytosolic Acetoacetyl-CoA Thiolase TaAACT1 Is Required for Defense against <i>Fusarium pseudograminearum</i> in Wheat |
| title_full | The Cytosolic Acetoacetyl-CoA Thiolase TaAACT1 Is Required for Defense against <i>Fusarium pseudograminearum</i> in Wheat |
| title_fullStr | The Cytosolic Acetoacetyl-CoA Thiolase TaAACT1 Is Required for Defense against <i>Fusarium pseudograminearum</i> in Wheat |
| title_full_unstemmed | The Cytosolic Acetoacetyl-CoA Thiolase TaAACT1 Is Required for Defense against <i>Fusarium pseudograminearum</i> in Wheat |
| title_short | The Cytosolic Acetoacetyl-CoA Thiolase TaAACT1 Is Required for Defense against <i>Fusarium pseudograminearum</i> in Wheat |
| title_sort | cytosolic acetoacetyl coa thiolase taaact1 is required for defense against i fusarium pseudograminearum i in wheat |
| topic | cytosolic acetoacetyl-CoA thiolase <i>Fusarium pseudograminearum</i> jasmonate plant defense wheat (<i>Triticum aestivum</i>) |
| url | https://www.mdpi.com/1422-0067/24/7/6165 |
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