Metabolomics Profiling Reveals Rehmanniae Radix Preparata Extract Protects against Glucocorticoid-Induced Osteoporosis Mainly via Intervening Steroid Hormone Biosynthesis
Rehmanniae Radix Preparata (RR), the dry rhizome of Rehmannia glutinosa Libosch., is a traditional herbal medicine for improving the liver and kidney function. Ample clinical and pharmacological experiments show that RR can prevent post-menopausal osteoporosis and senile osteoporosis. In the present...
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MDPI AG
2019-01-01
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| author | Tianshuang Xia Xin Dong Yiping Jiang Liuyue Lin Zhimin Dong Yi Shen Hailiang Xin Qiaoyan Zhang Luping Qin |
| author_facet | Tianshuang Xia Xin Dong Yiping Jiang Liuyue Lin Zhimin Dong Yi Shen Hailiang Xin Qiaoyan Zhang Luping Qin |
| author_sort | Tianshuang Xia |
| collection | DOAJ |
| description | Rehmanniae Radix Preparata (RR), the dry rhizome of Rehmannia glutinosa Libosch., is a traditional herbal medicine for improving the liver and kidney function. Ample clinical and pharmacological experiments show that RR can prevent post-menopausal osteoporosis and senile osteoporosis. In the present study, in vivo and in vitro experiments, as well as a UHPLC-Q/TOF-MS-based metabolomics study, were used to explore the preventing effect of RR on glucocorticoid-induced osteoporosis (GIOP) and its underlying mechanisms. As a result, RR significantly enhanced bone mineral density (BMD), improved the micro-architecture of trabecular bone, and intervened in biochemical markers of bone metabolism in dexamethasone (DEX)-treated rats. For the in vitro experiment, RR increased the cell proliferation and alkaline phosphatase (ALP) activity, enhanced the extracellular matrix mineralization level, and improved the expression of runt-related transcription factor 2 (RUNX2) and osteopontin (OPN) in DEX-injured osteoblasts. For the metabolomics study, a total of 27 differential metabolites were detected in the DEX group vs. the control group, of which 10 were significantly reversed after RR treatment. These metabolites were majorly involved in steroid hormone biosynthesis, sex steroids regulation, and amino acid metabolism. By metabolic pathway and Western blotting analysis, it was further ascertained that RR protected against DEX-induced bone loss, mainly via interfering steroid hormone biosynthesis, as evidenced by the up-regulation of cytochrome P450 17A1 (CYP17A1) and aromatase (CYP19A1), and the down-regulation of 11β-hydroxysteroid dehydrogenase (HSD11B1). Collectively, these results indicated that RR had a notable preventing effect on GIOP, and the action mechanism might be related to steroid hormone biosynthesis. |
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| spelling | doaj.art-6633ee8e3a5f4297b05170edd066f4c82022-12-22T01:46:20ZengMDPI AGMolecules1420-30492019-01-0124225310.3390/molecules24020253molecules24020253Metabolomics Profiling Reveals Rehmanniae Radix Preparata Extract Protects against Glucocorticoid-Induced Osteoporosis Mainly via Intervening Steroid Hormone BiosynthesisTianshuang Xia0Xin Dong1Yiping Jiang2Liuyue Lin3Zhimin Dong4Yi Shen5Hailiang Xin6Qiaoyan Zhang7Luping Qin8Department of Pharmacognosy, Second Military Medical University School of Pharmacy, Shanghai 200433, ChinaDepartment of Pharmacognosy, Second Military Medical University School of Pharmacy, Shanghai 200433, ChinaDepartment of Pharmacognosy, Second Military Medical University School of Pharmacy, Shanghai 200433, ChinaDepartment of Pharmacognosy, Second Military Medical University School of Pharmacy, Shanghai 200433, ChinaDepartment of Pharmacognosy, Second Military Medical University School of Pharmacy, Shanghai 200433, ChinaDepartment of Pharmacognosy, Second Military Medical University School of Pharmacy, Shanghai 200433, ChinaDepartment of Pharmacognosy, Second Military Medical University School of Pharmacy, Shanghai 200433, ChinaDepartment of Pharmacognosy, Second Military Medical University School of Pharmacy, Shanghai 200433, ChinaDepartment of Pharmacognosy, Second Military Medical University School of Pharmacy, Shanghai 200433, ChinaRehmanniae Radix Preparata (RR), the dry rhizome of Rehmannia glutinosa Libosch., is a traditional herbal medicine for improving the liver and kidney function. Ample clinical and pharmacological experiments show that RR can prevent post-menopausal osteoporosis and senile osteoporosis. In the present study, in vivo and in vitro experiments, as well as a UHPLC-Q/TOF-MS-based metabolomics study, were used to explore the preventing effect of RR on glucocorticoid-induced osteoporosis (GIOP) and its underlying mechanisms. As a result, RR significantly enhanced bone mineral density (BMD), improved the micro-architecture of trabecular bone, and intervened in biochemical markers of bone metabolism in dexamethasone (DEX)-treated rats. For the in vitro experiment, RR increased the cell proliferation and alkaline phosphatase (ALP) activity, enhanced the extracellular matrix mineralization level, and improved the expression of runt-related transcription factor 2 (RUNX2) and osteopontin (OPN) in DEX-injured osteoblasts. For the metabolomics study, a total of 27 differential metabolites were detected in the DEX group vs. the control group, of which 10 were significantly reversed after RR treatment. These metabolites were majorly involved in steroid hormone biosynthesis, sex steroids regulation, and amino acid metabolism. By metabolic pathway and Western blotting analysis, it was further ascertained that RR protected against DEX-induced bone loss, mainly via interfering steroid hormone biosynthesis, as evidenced by the up-regulation of cytochrome P450 17A1 (CYP17A1) and aromatase (CYP19A1), and the down-regulation of 11β-hydroxysteroid dehydrogenase (HSD11B1). Collectively, these results indicated that RR had a notable preventing effect on GIOP, and the action mechanism might be related to steroid hormone biosynthesis.http://www.mdpi.com/1420-3049/24/2/253Rehmanniae Radix Preparataglucocorticoid-induced osteoporosismetabolomicsosteoblaststeroid hormone biosynthesis |
| spellingShingle | Tianshuang Xia Xin Dong Yiping Jiang Liuyue Lin Zhimin Dong Yi Shen Hailiang Xin Qiaoyan Zhang Luping Qin Metabolomics Profiling Reveals Rehmanniae Radix Preparata Extract Protects against Glucocorticoid-Induced Osteoporosis Mainly via Intervening Steroid Hormone Biosynthesis Molecules Rehmanniae Radix Preparata glucocorticoid-induced osteoporosis metabolomics osteoblast steroid hormone biosynthesis |
| title | Metabolomics Profiling Reveals Rehmanniae Radix Preparata Extract Protects against Glucocorticoid-Induced Osteoporosis Mainly via Intervening Steroid Hormone Biosynthesis |
| title_full | Metabolomics Profiling Reveals Rehmanniae Radix Preparata Extract Protects against Glucocorticoid-Induced Osteoporosis Mainly via Intervening Steroid Hormone Biosynthesis |
| title_fullStr | Metabolomics Profiling Reveals Rehmanniae Radix Preparata Extract Protects against Glucocorticoid-Induced Osteoporosis Mainly via Intervening Steroid Hormone Biosynthesis |
| title_full_unstemmed | Metabolomics Profiling Reveals Rehmanniae Radix Preparata Extract Protects against Glucocorticoid-Induced Osteoporosis Mainly via Intervening Steroid Hormone Biosynthesis |
| title_short | Metabolomics Profiling Reveals Rehmanniae Radix Preparata Extract Protects against Glucocorticoid-Induced Osteoporosis Mainly via Intervening Steroid Hormone Biosynthesis |
| title_sort | metabolomics profiling reveals rehmanniae radix preparata extract protects against glucocorticoid induced osteoporosis mainly via intervening steroid hormone biosynthesis |
| topic | Rehmanniae Radix Preparata glucocorticoid-induced osteoporosis metabolomics osteoblast steroid hormone biosynthesis |
| url | http://www.mdpi.com/1420-3049/24/2/253 |
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