Unraveling plant adaptation to nitrogen limitation from enzyme stoichiometry aspect in Karst soils: a case study of Rhododendron Pudingense
Enzyme stoichiometry can reflect the resource limitation of soil microbial metabolism, and research on the relationships between plants and resource limitation in Karst Microhabitats is scarcely investigated. To clarify the extracellular enzyme stoichiometry characteristics in soil across different...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-11-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1267759/full |
| _version_ | 1797447714798043136 |
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| author | Haodong Wang Haodong Wang Haodong Wang Baoxian Huang Hongjiu Zhao Xiaoyong Dai Meng Chen Fangjun Ding Fangjun Ding Peng Wu Peng Wu Lei Hao Rui Yang Congjun Yuan Congjun Yuan |
| author_facet | Haodong Wang Haodong Wang Haodong Wang Baoxian Huang Hongjiu Zhao Xiaoyong Dai Meng Chen Fangjun Ding Fangjun Ding Peng Wu Peng Wu Lei Hao Rui Yang Congjun Yuan Congjun Yuan |
| author_sort | Haodong Wang |
| collection | DOAJ |
| description | Enzyme stoichiometry can reflect the resource limitation of soil microbial metabolism, and research on the relationships between plants and resource limitation in Karst Microhabitats is scarcely investigated. To clarify the extracellular enzyme stoichiometry characteristics in soil across different karst microhabitats and how the Rhododendron pudingense adapts to nutrient restrictions, plot investigation experiments were set up in Zhenning County, Qinglong County, and Wangmo County of Guizhou Province which included total three karst microhabitats, i.e., soil surface (SS), rock gully (RG), and rock surface (RS), by analyzing he rhizosphere soil nutrient, extracellular enzyme activity, and nutrient content of R. pudingense. The findings indicated that all karst microenvironments experienced varying levels of nitrogen (N) limitation, with the order of N limitation being as follows: SS > RG > RS. Notably, there were significant discrepancies in N content among different plant organs (p< 0.05), with the sequence of N content as follows: leaf > stem > root. However, no significant differences were observed in nutrient content within the same organ across different microenvironments (p > 0.05). A noteworthy discovery was the significant allometric growth relationship between C-P in various organs (p< 0.05), while roots and stems exhibited a significant allometric growth relationship between N-P (p< 0.05). The study highlighted the substantial impact of Total Nitrogen (TN) and N-acquiring enzymes (NAE) on nutrient allocation within the components of R. pudingense. Overall, the research demonstrated that N was the primary limiting factor in the study area’s soil, and R. pudingense’s nutrient allocation strategy was closely associated with N limitations in the karst microenvironment. Specifically, the plant prioritized allocating its limited N resources to its leaves, ensuring its survival. This investigation provided valuable insights into how plants adapt to nutrient restrictions and offered a deeper understanding of soil-plant interactions in karst ecosystems. |
| first_indexed | 2024-03-09T13:59:55Z |
| format | Article |
| id | doaj.art-6635978b423547d4af5e6b965b6bdee8 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-03-09T13:59:55Z |
| publishDate | 2023-11-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-6635978b423547d4af5e6b965b6bdee82023-11-30T08:47:01ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-11-011410.3389/fpls.2023.12677591267759Unraveling plant adaptation to nitrogen limitation from enzyme stoichiometry aspect in Karst soils: a case study of Rhododendron PudingenseHaodong Wang0Haodong Wang1Haodong Wang2Baoxian Huang3Hongjiu Zhao4Xiaoyong Dai5Meng Chen6Fangjun Ding7Fangjun Ding8Peng Wu9Peng Wu10Lei Hao11Rui Yang12Congjun Yuan13Congjun Yuan14College of Forestry, Guizhou University, Guiyang, ChinaGuizhou Academy of Forestry, Guiyang, ChinaNational Positioning Observation and Research Station of Guizhou Libo Karst Forest Ecosystem, Libo, Guizhou, ChinaCollege of Forestry, Guizhou University, Guiyang, ChinaCollege of Forestry, Guizhou University, Guiyang, ChinaGuizhou Academy of Forestry, Guiyang, ChinaGuizhou Academy of Forestry, Guiyang, ChinaGuizhou Academy of Forestry, Guiyang, ChinaNational Positioning Observation and Research Station of Guizhou Libo Karst Forest Ecosystem, Libo, Guizhou, ChinaGuizhou Academy of Forestry, Guiyang, ChinaNational Positioning Observation and Research Station of Guizhou Libo Karst Forest Ecosystem, Libo, Guizhou, ChinaGuizhou Provincial Institute of Biology, Guiyang, Guizhou, ChinaCollege of Forestry, Guizhou University, Guiyang, ChinaGuizhou Academy of Forestry, Guiyang, ChinaNational Positioning Observation and Research Station of Guizhou Libo Karst Forest Ecosystem, Libo, Guizhou, ChinaEnzyme stoichiometry can reflect the resource limitation of soil microbial metabolism, and research on the relationships between plants and resource limitation in Karst Microhabitats is scarcely investigated. To clarify the extracellular enzyme stoichiometry characteristics in soil across different karst microhabitats and how the Rhododendron pudingense adapts to nutrient restrictions, plot investigation experiments were set up in Zhenning County, Qinglong County, and Wangmo County of Guizhou Province which included total three karst microhabitats, i.e., soil surface (SS), rock gully (RG), and rock surface (RS), by analyzing he rhizosphere soil nutrient, extracellular enzyme activity, and nutrient content of R. pudingense. The findings indicated that all karst microenvironments experienced varying levels of nitrogen (N) limitation, with the order of N limitation being as follows: SS > RG > RS. Notably, there were significant discrepancies in N content among different plant organs (p< 0.05), with the sequence of N content as follows: leaf > stem > root. However, no significant differences were observed in nutrient content within the same organ across different microenvironments (p > 0.05). A noteworthy discovery was the significant allometric growth relationship between C-P in various organs (p< 0.05), while roots and stems exhibited a significant allometric growth relationship between N-P (p< 0.05). The study highlighted the substantial impact of Total Nitrogen (TN) and N-acquiring enzymes (NAE) on nutrient allocation within the components of R. pudingense. Overall, the research demonstrated that N was the primary limiting factor in the study area’s soil, and R. pudingense’s nutrient allocation strategy was closely associated with N limitations in the karst microenvironment. Specifically, the plant prioritized allocating its limited N resources to its leaves, ensuring its survival. This investigation provided valuable insights into how plants adapt to nutrient restrictions and offered a deeper understanding of soil-plant interactions in karst ecosystems.https://www.frontiersin.org/articles/10.3389/fpls.2023.1267759/fullKarst microhabitatenzyme stoichiometryRhododendron pudingensenutrient allocationN limitation |
| spellingShingle | Haodong Wang Haodong Wang Haodong Wang Baoxian Huang Hongjiu Zhao Xiaoyong Dai Meng Chen Fangjun Ding Fangjun Ding Peng Wu Peng Wu Lei Hao Rui Yang Congjun Yuan Congjun Yuan Unraveling plant adaptation to nitrogen limitation from enzyme stoichiometry aspect in Karst soils: a case study of Rhododendron Pudingense Frontiers in Plant Science Karst microhabitat enzyme stoichiometry Rhododendron pudingense nutrient allocation N limitation |
| title | Unraveling plant adaptation to nitrogen limitation from enzyme stoichiometry aspect in Karst soils: a case study of Rhododendron Pudingense |
| title_full | Unraveling plant adaptation to nitrogen limitation from enzyme stoichiometry aspect in Karst soils: a case study of Rhododendron Pudingense |
| title_fullStr | Unraveling plant adaptation to nitrogen limitation from enzyme stoichiometry aspect in Karst soils: a case study of Rhododendron Pudingense |
| title_full_unstemmed | Unraveling plant adaptation to nitrogen limitation from enzyme stoichiometry aspect in Karst soils: a case study of Rhododendron Pudingense |
| title_short | Unraveling plant adaptation to nitrogen limitation from enzyme stoichiometry aspect in Karst soils: a case study of Rhododendron Pudingense |
| title_sort | unraveling plant adaptation to nitrogen limitation from enzyme stoichiometry aspect in karst soils a case study of rhododendron pudingense |
| topic | Karst microhabitat enzyme stoichiometry Rhododendron pudingense nutrient allocation N limitation |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1267759/full |
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