Molecular detection of atypical microorganisms in patients with ventilator associated pneumonia
Ventilator-associated pneumonia (VAP) is one of the major causes of morbidity and mortality among the critically ill patients of intensive care units (ICU). The present cross sectional study was conducted to isolate and identify bacterial causes of VAP among the patients admitted in intensive care u...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Ibrahim Medical College
2015-01-01
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| Series: | IMC Journal of Medical Science |
| Online Access: | http://www.imcjms.com/registration/journal_full_text/252 |
| _version_ | 1819207666871828480 |
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| author | Shahida Akter Rehana Khatun S.M Shamsuzzaman |
| author_facet | Shahida Akter Rehana Khatun S.M Shamsuzzaman |
| author_sort | Shahida Akter |
| collection | DOAJ |
| description | Ventilator-associated pneumonia (VAP) is one of the major causes of morbidity and mortality among the critically ill patients of intensive care units (ICU). The present cross sectional study was conducted to isolate and identify bacterial causes of VAP among the patients admitted in intensive care unit (ICU) of Dhaka Medical College Hospital. The study was conducted between July, 2013 to June 2014. A total of 65 endotracheal aspirate (ETA) and blood samples were collected from patients with clinically suspected ventilator associated peumonia(VAP). Samples were collected from patients on mechanical ventilation for more than 48 hours. ETA and blood samples were cultured aerobically. Multiplex PCR was performed with ETA to detect Mycoplasma pneumoniae, Legionella pneumophila and Chlamydia pneumoniae. Among the atypical bacteria, M. pneumoniae were detected in 5 (7.69%), L. pneumophila in 4 (6.15%) cases by multiplex PCR in ETA from VAP cases. No C. pneumoniae was detected. The study revealed that in VAP cases atypical bacteria should be considered as a possible bacterial agents.
Ibrahim Med. Coll. J. 2015; 9(1): 22-25 |
| first_indexed | 2024-12-23T05:27:08Z |
| format | Article |
| id | doaj.art-663656dd9e024af5b3ce14248efeaf25 |
| institution | Directory Open Access Journal |
| issn | 2519-1721 2519-1586 |
| language | English |
| last_indexed | 2024-12-23T05:27:08Z |
| publishDate | 2015-01-01 |
| publisher | Ibrahim Medical College |
| record_format | Article |
| series | IMC Journal of Medical Science |
| spelling | doaj.art-663656dd9e024af5b3ce14248efeaf252022-12-21T17:58:34ZengIbrahim Medical CollegeIMC Journal of Medical Science2519-17212519-15862015-01-01912225Molecular detection of atypical microorganisms in patients with ventilator associated pneumoniaShahida Akter0Rehana Khatun1S.M Shamsuzzaman 2Department of Microbiology,Dhaka Medical College,DhakaDepartment of Microbiology,Dhaka Medical College,DhakaDepartment of Microbiology,Dhaka Medical College,DhakaVentilator-associated pneumonia (VAP) is one of the major causes of morbidity and mortality among the critically ill patients of intensive care units (ICU). The present cross sectional study was conducted to isolate and identify bacterial causes of VAP among the patients admitted in intensive care unit (ICU) of Dhaka Medical College Hospital. The study was conducted between July, 2013 to June 2014. A total of 65 endotracheal aspirate (ETA) and blood samples were collected from patients with clinically suspected ventilator associated peumonia(VAP). Samples were collected from patients on mechanical ventilation for more than 48 hours. ETA and blood samples were cultured aerobically. Multiplex PCR was performed with ETA to detect Mycoplasma pneumoniae, Legionella pneumophila and Chlamydia pneumoniae. Among the atypical bacteria, M. pneumoniae were detected in 5 (7.69%), L. pneumophila in 4 (6.15%) cases by multiplex PCR in ETA from VAP cases. No C. pneumoniae was detected. The study revealed that in VAP cases atypical bacteria should be considered as a possible bacterial agents. Ibrahim Med. Coll. J. 2015; 9(1): 22-25http://www.imcjms.com/registration/journal_full_text/252 |
| spellingShingle | Shahida Akter Rehana Khatun S.M Shamsuzzaman Molecular detection of atypical microorganisms in patients with ventilator associated pneumonia IMC Journal of Medical Science |
| title | Molecular detection of atypical microorganisms in patients with ventilator associated pneumonia |
| title_full | Molecular detection of atypical microorganisms in patients with ventilator associated pneumonia |
| title_fullStr | Molecular detection of atypical microorganisms in patients with ventilator associated pneumonia |
| title_full_unstemmed | Molecular detection of atypical microorganisms in patients with ventilator associated pneumonia |
| title_short | Molecular detection of atypical microorganisms in patients with ventilator associated pneumonia |
| title_sort | molecular detection of atypical microorganisms in patients with ventilator associated pneumonia |
| url | http://www.imcjms.com/registration/journal_full_text/252 |
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